Inducing Autophagy and Blocking Autophagic Flux via a Virus-Mimicking Nanodrug for Cancer Therapy

Li, Xiao; Wang, Zhi-Gang; Zhu, Han; Wen, Hui-Ping; Ning, Di; Liu, Hao-Yang; Pang, Dai‐Wen; Liu, Shu Lin

doi:10.1021/acs.nanolett.2c04091

Cited by 13 publications

(11 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8). 119 This nanodrug comprised a JEV mimic virion decorated with anti-PDL1 antibodies and encapsulated with hydroxychloroquine (HCQ), an autophagy inhibitor widely used in clinical practice. In vivo studies have demonstrated that this nanodrug effectively upregulates cellular autophagy, inhibits autophagic flux, induces tumor cell apoptosis, and enhances immune response.…”

Section: Virus-mimicking Nanosystems For Biomedical Applicationsmentioning

confidence: 99%

Virus-mimicking nanosystems: from design to biomedical applications

Liu,

Li,

Wang

et al. 2023

Chem. Soc. Rev.

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Virus-mimicking Nanosystems For Biomedical Applicationsmentioning

confidence: 99%

Virus-mimicking nanosystems: from design to biomedical applications

Liu,

Li,

Wang

et al. 2023

Chem. Soc. Rev.

Self Cite

View full text Add to dashboard Cite

show abstract

“…This is because LysoSensor probes rely on the decreased pH values to sense the endo-lysosomal compartments, and the protons that create a low pH environment are released into the cytoplasm upon the compartment rupture 36 , 37 . Moreover, LysoSensor probes exhibit a pH-dependent increase in fluorescence intensity and are almost non-fluorescent when outside acidic compartments 38 . Based on this, we studied the integrity of endo-lysosomal compartments by measuring the fluorescence intensity of LysoSensor Green.…”

Section: Resultsmentioning

confidence: 99%

Nanomechanical action opens endo-lysosomal compartments

Zhao,

Ye,

Song

et al. 2023

Nat Commun

View full text Add to dashboard Cite

Endo-lysosomal escape is a highly inefficient process, which is a bottleneck for intracellular delivery of biologics, including proteins and nucleic acids. Herein, we demonstrate the design of a lipid-based nanoscale molecular machine, which achieves efficient cytosolic transport of biologics by destabilizing endo-lysosomal compartments through nanomechanical action upon light irradiation. We fabricate lipid-based nanoscale molecular machines, which are designed to perform mechanical movement by consuming photons, by co-assembling azobenzene lipidoids with helper lipids. We show that lipid-based nanoscale molecular machines adhere onto the endo-lysosomal membrane after entering cells. We demonstrate that continuous rotation-inversion movement of Azo lipidoids triggered by ultraviolet/visible irradiation results in the destabilization of the membranes, thereby transporting cargoes, such as mRNAs and Cre proteins, to the cytoplasm. We find that the efficiency of cytosolic transport is improved about 2.1-fold, compared to conventional intracellular delivery systems. Finally, we show that lipid-based nanoscale molecular machines are competent for cytosolic transport of tumour antigens into dendritic cells, which induce robust antitumour activity in a melanoma mouse model.

show abstract

“…Lysosomal escape through JEV promoted the drug aggregation in tumors, inducing autophagy while inhibiting autophagolysosome by HCQ, which significantly enhanced the therapeutic effect on breast cancer. 192 …”

Section: Application Of Nanomedicine Combined With Autophagy Regulato...mentioning

confidence: 99%

Nanoplatform-Mediated Autophagy Regulation and Combined Anti-Tumor Therapy for Resistant Tumors

Yang,

Ding,

Mao

et al. 2024

IJN

View full text Add to dashboard Cite

The overall cancer incidence and death toll have been increasing worldwide. However, the conventional therapies have some obvious limitations, such as non-specific targeting, systemic toxic effects, especially the multidrug resistance (MDR) of tumors, in which, autophagy plays a vital role. Therefore, there is an urgent need for new treatments to reduce adverse reactions, improve the treatment efficacy and expand their therapeutic indications more effectively and accurately. Combination therapy based on autophagy regulators is a very feasible and important method to overcome tumor resistance and sensitize anti-tumor drugs. However, the less improved efficacy, more systemic toxicity and other problems limit its clinical application. Nanotechnology provides a good way to overcome this limitation. Co-delivery of autophagy regulators combined with anti-tumor drugs through nanoplatforms provides a good therapeutic strategy for the treatment of tumors, especially drug-resistant tumors. Notably, the nanomaterials with autophagy regulatory properties have broad therapeutic prospects as carrier platforms, especially in adjuvant therapy. However, further research is still necessary to overcome the difficulties such as the safety, biocompatibility, and side effects of nanomedicine. In addition, clinical research is also indispensable to confirm its application in tumor treatment.

show abstract

Inducing Autophagy and Blocking Autophagic Flux via a Virus-Mimicking Nanodrug for Cancer Therapy

Cited by 13 publications

References 42 publications

Virus-mimicking nanosystems: from design to biomedical applications

Virus-mimicking nanosystems: from design to biomedical applications

Nanomechanical action opens endo-lysosomal compartments

Nanoplatform-Mediated Autophagy Regulation and Combined Anti-Tumor Therapy for Resistant Tumors

Contact Info

Product

Resources

About