Inducing hyperosmotic stress resistance in succinate-producing Escherichia coli by using the response regulator DR1558 from Deinococcus radiodurans

Guo, Shunfeng; Yi, Xiangyan; Zhang, Wenming; Wu, Mingke; Xin, Fengxue; Dong, Weiliang; Zhang, Min; Ma, Jiangfeng; Wu, Hao; Jiang, Min

doi:10.1016/j.procbio.2017.06.002

Cited by 15 publications

(9 citation statements)

References 44 publications

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“…One study has employed DR1558 for increasing the tolerance of E. coli against low pH, heat, and salt stresses [35]. Additionally, DR1558 has been used to enhance cell robustness and production of succinate, 2,3-butanediol (2,3-BDO) and poly(3-hydroxybutyrate) [P(3HB)] in the recombinant E. coli strains [36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Effect of DR1558, a Deinococcus radiodurans response regulator, on the production of GABA in the recombinant Escherichia coli under low pH conditions

et al. 2020

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Background: Gamma aminobutyric acid (GABA) is an important platform chemical, which has been used as a food additive and drug. Additionally, GABA is a precursor of 2-pyrrolidone, which is used in nylon synthesis. GABA is usually synthesized from glutamate in a reaction catalyzed by glutamate decarboxylase (GAD). Currently, there are several reports on GABA production from monosodium glutamate (MSG) or glucose using engineered microbes. However, the optimal pH for GAD activity is 4, which is the limiting factor for the efficient microbial fermentative production of GABA as fermentations are performed at pH 7. Recently, DR1558, a response regulator in the two-component signal transduction system was identified in Deinococcus radiodurans. DR1558 is reported to confer cellular robustness to cells by binding the promoter regions of genes via DNA-binding domains or by binding to the effector molecules, which enable the microorganisms to survive in various environmental stress conditions, such as oxidative stress, high osmotic shock, and low pH. Results: In this study, the effect of DR1558 in enhancing GABA production was examined using two different strategies: whole-cell bioconversion of GABA from MSG and direct fermentative production of GABA from glucose under acidic culture conditions. In the whole-cell bioconversion, GABA produced by E. coli expressing GadBC and DR1558 (6.52 g/L GABA from 13 g/L MSG•H 2 O) in shake flask culture at pH 4.5 was 2.2-fold higher than that by E. coli expressing only GadBC (2.97 g/L of GABA from 13 g/L MSG•H 2 O). In direct fermentative production of GABA from glucose, E. coli ∆gabT expressing isocitrate dehydrogenase (IcdA), glutamate dehydrogenase (GdhA), GadBC, and DR1558 produced 1.7-fold higher GABA (2.8 g/L of GABA from 30 g/L glucose) than E. coli ∆gabT expressing IcdA, GdhA, and GadBC (1.6 g/L of GABA from 30 g/L glucose) in shake flask culture at an initial pH 7.0. The transcriptional analysis of E. coli revealed that DR1558 conferred acid resistance to E. coli during GABA production. The fed-batch fermentation of E. coli expressing IcdA, GdhA, GadBC, and DR1558 performed at pH 5.0 resulted in the final GABA titer of 6.16 g/L by consuming 116.82 g/L of glucose in 38 h. Conclusion: This is the first report to demonstrate GABA production by acidic fermentation and to provide an engineering strategy for conferring acid resistance to the recombinant E. coli for GABA production.

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Section: Introductionmentioning

confidence: 99%

Effect of DR1558, a Deinococcus radiodurans response regulator, on the production of GABA in the recombinant Escherichia coli under low pH conditions

et al. 2020

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“…19 By overexpression of the response regulator in two-component signal transduction systems, DR1558 from D. radiodurans, recombinant E. coli showed better performance in the presence of osmotic stress as well as high concentrations of 300 g L −1 glucose and 2 mol L −1 NaCl. 20 Expressing the native fucO or mutations also described a novel approach to obtain furfural tolerance strains, which doubled the rate of furfural metabolism during fermentation. 21 Synthetic biological approaches can also be combined with other new emerging strategies to enhance the acidic resistance through the design of several unique operons composed of diverse combinations of three novel genes from an extreme environment and three synthetic ribosome binding sites.…”

Section: Transcription Factorsmentioning

confidence: 99%

“…This also provided a research foundation for DNA microarray analysis and resulted in the discovery of several important regulation genes 19 . By overexpression of the response regulator in two‐component signal transduction systems, DR1558 from D. radiodurans , recombinant E. coli showed better performance in the presence of osmotic stress as well as high concentrations of 300 g L −1 glucose and 2 mol L −1 NaCl 20 . Expressing the native fucO or mutations also described a novel approach to obtain furfural tolerance strains, which doubled the rate of furfural metabolism during fermentation 21 …”

Section: Regulation Factors Engineeringmentioning

confidence: 99%

Strategies to improve the stress resistance of Escherichia coli in industrial biotechnology

Tao

Wang

et al. 2022

Biofuels Bioprod Bioref

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The use of industrial Escherichia coli strains for the production of bio‐based chemicals offers a promising and sustainable solution to a growing scarcity of non‐renewable resources and contributes to the protection of the environment. However, many limitations, such as inhibition from toxic substrates, hyperosmosis, toxic by‐products, and other adverse fermentation conditions, prevent strains from attaining a stable cell growth rate, efficient metabolic flux, and satisfactory productivity. There has thus been much effort to obtain E. coli strains that exhibit robust growth and productivity with enhanced tolerance of extreme operating conditions. In this review, we gained insights into recent studies in improving tolerance of E. coli and summarized four kinds of engineering methods: modifying regulation factors, regulating membrane structure, optimizing biosynthetic pathways, and applying adaptive evolution. The combination of different engineering strategies therefore provided a broader platform for robustness of industrial E. coli, making further steps to achieving more advantageous biosynthesis. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…Biosynthesis of adipic acid, an important platform chemical in industry, has also been realized in engineered E. coli [ 87 ]. Importantly, plenty of alkali was added to maintain the neutral conditions during microbial fermentation, increasing the osmotic pressure of the fermentation broth and inhibiting the performance of cell growth and metabolism [ 88 ]. Moreover, the cost of production and separation will increase.…”

Section: Introductionmentioning

confidence: 99%

Current advance in bioconversion of methanol to chemicals

Zhang

Song

Qiao

et al. 2018

Biotechnol Biofuels

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Methanol has become an attractive substrate for biotechnological applications due to its abundance and low-price. Chemicals production from methanol could alleviate the environmental concerns, costs, and foreign dependency associated with the use of petroleum feedstock. Recently, a growing fraction of research has focused on metabolites production using methanol as sole carbon and energy source or as co-substrate with carbohydrates by native or synthetic methylotrophs. In this review, we summarized the recent significant progress in native and synthetic methylotrophs and their application for methanol bioconversion into various products. Moreover, strategies for improvement of methanol metabolism and new perspectives on the generation of desired products from methanol were also discussed, which will benefit for the development of a methanol-based economy.

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Inducing hyperosmotic stress resistance in succinate-producing Escherichia coli by using the response regulator DR1558 from Deinococcus radiodurans

Cited by 15 publications

References 44 publications

Effect of DR1558, a Deinococcus radiodurans response regulator, on the production of GABA in the recombinant Escherichia coli under low pH conditions

Effect of DR1558, a Deinococcus radiodurans response regulator, on the production of GABA in the recombinant Escherichia coli under low pH conditions

Strategies to improve the stress resistance of Escherichia coli in industrial biotechnology

Current advance in bioconversion of methanol to chemicals

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