Induction and differentiation of adipose-derived stem cells from human buccal fat pads into salivary gland cells

Kawakami, Miyuki; Ishikawa, Hiroshi; Tanaka, Akira; Mataga, Izumi

doi:10.1007/s13577-016-0132-z

Cited by 18 publications

(13 citation statements)

References 31 publications

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“…In the present study, significant intergroup differences were observed for body and SG weights. In a previous study, RI exposed SGs showed atrophy of parenchyma, tissue necrosis, and fibrosis resulting from inflammation 17 , and in another, it was suggested simultaneous AdMSCs transplantation might protect SG cells from atrophied and act to regenerate acinar cells and restore saliva volume 22 . In the present study, salivary lag times were shorter and salivary flow rates were significantly higher in the RI + AdMSCs group than in the RI + PBS sham group, which also suggests AdMSCs protect SG cells against RI.…”

Section: Discussionmentioning

confidence: 95%

Adipose-derived mesenchymal stem cells regenerate radioiodine-induced salivary gland damage in a murine model

Kim

Choi

et al. 2019

Sci Rep

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After radioiodine (RI) therapy, patients with thyroid cancer frequently suffer from painful salivary gland (SG) swelling, xerostomia, taste alterations, and oral infections. This study was aimed to determine whether adipose-derived mesenchymal stem cells (AdMSCs) might restore RI-induced SG dysfunction in a murine model. Forty -five mice were divided into three groups; a PBS sham group, a RI+ PBS sham group (0.01 mCi/g mouse, orally), and an RI+AdMSCs (1 × 105 cells/150 uL, intraglandular injection on experimental day 28) treated group. At 16 weeks after RI treatment, body weights, SG weight, salivary flow rates (SFRs), and salivary lag times were measured. Morphologic and histologic examinations and immunohistochemistry (IHC) were performed and the activities of amylase and EGF in saliva were also measured. Changes in salivary 99mTc pertechnetate excretion were followed by SPECT and TUNEL assays were performed. The body and SG weights were similar in the AdMSCs and sham groups. Hematoxylin and eosin staining revealed the AdMSCs group had more mucin-containing acini than the RI group. Furthermore, AdMSCs treatment resulted in tissue remodeling and elevated expressions of epithelial (AQP5) and endothelial (CD31) markers, and increased SFRs. The activities of amylase and EGF were higher in the AdMSCs group than in the RI treated group. 99mTc pertechnetate excretions were similar in the AdMSCs and sham group. Also, TUNEL positive apoptotic cell numbers were less in the AdMSCs group than in the RI group. Local delivery of AdMSCs might regenerate SG damage induced by RI.

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Section: Discussionmentioning

confidence: 95%

Adipose-derived mesenchymal stem cells regenerate radioiodine-induced salivary gland damage in a murine model

Kim

Choi

et al. 2019

Sci Rep

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“…(); g Farre‐Guasch, Marti‐Page, Hernadez‐Alfaro, Klein‐Nulend, and Casals (); h Broccaioli et al. (); i Kawakami, Ishikawa, Tanaka, and Mataga ().…”

Section: Characteristics Of Human Dedifferentiated Fat Cells—in Vitromentioning

confidence: 99%

“…+Means cell is positive for this CD marker; -means cell is negative for this CD marker. The superscript letter next to the + or -sign refers to the study that reported that finding: aKou et al (2014); b Tsurumachi et al (2016); c Kishimoto et al (2014); d Matsumoto et al (2008); e Watson et al (2014); f Poloni et al (2012); g Farre-Guasch, Marti-Page, Hernadez-Alfaro, Klein-Nulend, and Casals (2010); h Broccaioli et al (2013); iKawakami, Ishikawa, Tanaka, and Mataga (2016).…”

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confidence: 99%

Dedifferentiated Fat (DFAT) cells: A cell source for oral and maxillofacial tissue engineering

et al. 2018

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Tissue engineering is a promising method for the regeneration of oral and maxillofacial tissues. Proper selection of a cell source is important for the desired application. This review describes the discovery and usefulness of dedifferentiated fat (DFAT) cells as a cell source for tissue engineering. Dedifferentiated Fat cells are a highly homogeneous cell population (high purity), highly proliferative, and possess a multilineage potential for differentiation into various cell types under proper in vitro inducing conditions and in vivo. Moreover, DFAT cells have a higher differentiation capability of becoming osteoblasts, chondrocytes, and adipocytes than do bone marrow-derived mesenchymal stem cells and/or adipose tissue-derived stem cells. The usefulness of DFAT cells in vivo for periodontal tissue, bone, peripheral nerve, muscle, cartilage, and fat tissue regeneration was reported. Dedifferentiated Fat cells obtained from the human buccal fat pad (BFP) are a minimally invasive procedure with limited esthetic complications for patients. The BFP is a convenient and accessible anatomical site to harvest DFAT cells for dentists and oral surgeons, and thus is a promising cell source for oral and maxillofacial tissue engineering.

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“…Both animal and human experiments have verified that ADSCs could represent a safe treatment strategy for salivary gland damage[ 40 , 114 , 115 , 117 , 120 , 123 ].…”

Section: Salivary Gland Damagementioning

confidence: 99%

Adipose-derived stromal cell in regenerative medicine: A review

Qomi

Sheykhhasan

2017

WJSC

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The application of appropriate cell origin for utilizing in regenerative medicine is the major issue. Various kinds of stem cells have been used for the tissue engineering and regenerative medicine. Such as, several stromal cells have been employed as treat option for regenerative medicine. For example, human bone marrow-derived stromal cells and adipose-derived stromal cells (ADSCs) are used in cell-based therapy. Data relating to the stem cell therapy and processes associated with ADSC has developed remarkably in the past 10 years. As medical options, both the stromal vascular and ADSC suggests good opportunity as marvelous cell-based therapeutics. The some biological features are the main factors that impact the regenerative activity of ADSCs, including the modulation of the cellular immune system properties and secretion of bioactive proteins such as cytokines, chemokines and growth factors, as well as their intrinsic anti-ulcer and anti-inflammatory potential. A variety of diseases have been treated by ADSCs, and it is not surprising that there has been great interest in the possibility that ADSCs might be used as therapeutic strategy to improve a wider range of diseases. This is especially important when it is remembered that routine therapeutic methods are not completely effective in treat of diseases. Here, it was discuss about applications of ADSC to colitis, liver failure, diabetes mellitus, multiple sclerosis, orthopaedic disorders, hair loss, fertility problems, and salivary gland damage.

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Induction and differentiation of adipose-derived stem cells from human buccal fat pads into salivary gland cells

Cited by 18 publications

References 31 publications

Adipose-derived mesenchymal stem cells regenerate radioiodine-induced salivary gland damage in a murine model

Adipose-derived mesenchymal stem cells regenerate radioiodine-induced salivary gland damage in a murine model

Dedifferentiated Fat (DFAT) cells: A cell source for oral and maxillofacial tissue engineering

Adipose-derived stromal cell in regenerative medicine: A review

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