Induction and metal speciation of metallothionein in wood mice (<i>Apodemus sylvaticus</i>) along a metal pollution gradient

Rogival, Damien; Campenhout, Karen Van; Infante, Heidi Goenaga; Hearn, Ruth; Scheirs, Jan; Blust, Ronny

doi:10.1897/06-351r1.1

Cited by 17 publications

(6 citation statements)

References 32 publications

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“…(affinity decreases in order Hg(II) > Ag(I) ~ Cu(I) > Cd(II) > Zn(II) [198]. These methods have been used for analysis of MT content in a wide range of species including water organisms [199][200][201][202][203][204][205][206][207], mice [208], rats [209] and in chicken chondrocytes [210]. The work aimed on MT determination in crustaceans using Ag-saturation method coupled with AAS detection of silver was described by Delramo et al demonstrating detection limit of 0.03 ppm [211].…”

Section: Saturation Assaysmentioning

confidence: 99%

Analytical Methods for Metallothionein Detection

Ryvolová¹,

Křížková²,

Adam³

et al. 2011

CAC

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Metallothioneins (MTs) are a group of low molecular mass, cysteine-rich proteins with a variety of functions including involvement in metal homeostasis, free radical scavenging, protection against heavy metal damage, and metabolic regulation via Zn donation. The overexpression of MTs in proliferating cells is turning the attention to the study of MTs as novel promising marker of tumor diseases. Besides, levels of MTs in invertebrates and aquatic vertebrates well correlate with heavy metal pollution of an environment and, thus, serve as bio-environmental marker. It is not surprising that these proteins are of great interest not only for biochemists, molecular biologists clinical chemists, but also for environmental chemists and ecologists.Detection and quantification of MTs, which is needed in all above mentioned areas, is challenging due to the high cysteine content and relatively low molecular mass. This review is giving an overview of the analytical methods used for determination of MTs comprising a broad range of immunochemical, electrochemical as well as spectrometric methods, which have been optimized and even hyphenated with different separation methods to detect MTs. More attention is paid to the structural analysis of these proteins as well as the employment of analytical instruments for environmental, biochemical and clinical purposes.

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Section: Saturation Assaysmentioning

confidence: 99%

Analytical Methods for Metallothionein Detection

Ryvolová¹,

Křížková²,

Adam³

et al. 2011

CAC

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“…The high proportion of Zn bound to proteins larger than 100 kDa in specimens from the field and fed with metals ( Fig. 1) has a parallel in the snail H. pomatia (Dallinger and Wieser 1984), the earthworm Dendrobaena rubida (Bengtsson et al 1992), and the wood mouse Apodemus sylvaticus (Rogival et al 2007) used in experiments or collected from contaminated gradients in the field. Especially specimens from uncontaminated habitats or fed uncontaminated food in these studies have much Zn bound to these large proteins, whereas Zn in exposed specimens also binds in larger quantities to various proteins smaller than 100 kDa, just like in Fig.…”

Section: Discussionmentioning

confidence: 94%

PIXE-electrophoresis shows starving collembolan reallocates protein-bound metals

Bengtsson

Pallon²,

Nilsson³

et al. 2015

Ecotoxicology

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One of multiple functions of metalloproteins is to provide detoxification to excess metal levels in organisms. Here we address the induction and persistence of a range of low to high molecular weight copper- and zinc binding proteins in the collembolan species Tetrodontophora bielanensis exposed to copper- and zinc-enriched food, followed by a period of recovery from metal exposure, in absence and presence of food. After 10 days of feeding copper and zinc contaminated yeast, specimens were either moved to ample of leaf litter material from their woodland stand of origin or starved (no food offered). The molecular weight distribution of metal binding proteins was determined by native polyacryl gel electrophoresis. One gel was stained with Comassie brilliant blue and a duplicate gel dried and scanned for the amount of copper and zinc by particle-induced X-ray emission. Specimens exposed to copper and recovered from it with ample of food had copper bound to two groups of rather low molecular weight proteins (40-50 kDa) and two of intermediate size (70-80 kDa). Most zinc in specimens from the woodland stand was bound to two large proteins of about 104 and 106 kDa. The same proteins were holding some zinc in metal-exposed specimens, but most zinc was found in proteins <40 kDa in size. Specimens recovered from metal exposure in presence of ample of food had the same distribution pattern of zinc binding proteins, whereas starved specimens had zinc as well as copper mainly bound to two proteins of 8 and 10 kDa in size. Thus, the induction and distribution of copper- and zinc-binding proteins depend on exposure conditions, and the presence of low molecular weight binding proteins, characteristic of metallothioneins, was mainly limited to starving conditions.

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“…Cadmium, ionizing radiation, glucocorticoids and cytokines belong to factors that increase significantly the production of metallothionein in the body (Rogival et al 2007).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Low Dose Ionizing Radiation and Cadmium Chloride on Glucose Metabolism in Broiler Chickens

et al. 2010

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The aim of the study was to observe the effect of ionizing radiation and cadmium chloride on glucose metabolism in broiler chickens. The experiment was conducted on 28-day-old chickens divided into four groups (A, B, C and D). The first group (A) served as control, groups 2 (B) and 3 (C) were irradiated with a single, whole body dose of gamma rays (3 Gy, input 1.14 Gy·min -1 ) and group 3 (C) was administered a water solution of CdCl 2 intraperitoneally at a dose of 6 mg·kg -1 body weight. Group 4 (D) was administered a water solution of CdCl 2 intraperitoneally at the same dose as group 3 but chickens of this group were not irradiated with gamma rays. The serum glucose concentration in the chickens that were irradiated and administered CdCl 2 solution (C) was decreased significantly (p < 0.05) on days 7, 14 and 21 (p < 0.01) of the experiment compared to control (A). The serum glucose concentration in the chickens administered only CdCl 2 (D) was decreased significantly on day 7 compared to control (A). No significant changes in serum glucose concentrations were observed in the remaining group. The results of our experiment indicate that a combined influence of ionizing radiation and administered cadmium chloride resulted in a significant decrease in serum glucose concentration and the influence was more evident than that caused either by cadmium chloride or irradiation alone. The changes in serum glucose concentration observed on days 7 and 14 in gamma-irradiated chickens were lower than those induced only by administration of cadmium chloride.

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Induction and metal speciation of metallothionein in wood mice (Apodemus sylvaticus) along a metal pollution gradient

Cited by 17 publications

References 32 publications

Analytical Methods for Metallothionein Detection

Analytical Methods for Metallothionein Detection

PIXE-electrophoresis shows starving collembolan reallocates protein-bound metals

The Effect of Low Dose Ionizing Radiation and Cadmium Chloride on Glucose Metabolism in Broiler Chickens

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