Induction and production of cellulases by l-sorbose in Trichoderma reesei

Kawamori, Mikio; Morikawa, Yasushi; Takasawa, Seigo

doi:10.1007/bf00250321

Cited by 49 publications

(48 citation statements)

References 12 publications

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“…Trichoderma reesei PC-3-7, a hypercellulolytic mutant strain, was used throughout this study. 37) A pyr4-negative mutant derived from T. reesei PC-3-7 was used as host in pyr4-mediated transformation experiments. The pyr4-negative mutants (uridine auxotrophs) were constructed by inserting the amdS gene from Aspergillus nidulans 38) into the coding region of pyr4 against two genetic backgrounds (Suda et al, unpublished data).…”

Section: Methodsmentioning

confidence: 99%

Identification of Major Facilitator Transporters Involved in Cellulase Production during Lactose Culture ofTrichoderma reeseiPC-3-7

Porciúncula

Furukawa

Shida

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Section: Methodsmentioning

confidence: 99%

Identification of Major Facilitator Transporters Involved in Cellulase Production during Lactose Culture ofTrichoderma reeseiPC-3-7

Porciúncula

Furukawa

Shida

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…reesei PC-3-7 (Kawamori et al, 1986) and a xyr1-knock out strain were used throughout this study. T. reesei PC-3-7 is a cellulase-hyperproducing mutant that exhibits enhanced cellulase and xylanase inducibility by L-sorbose (Kawamori et al, 1986;Nogawa et al, 2001). These strains were maintained on Potato Dextrose agar slants.…”

Section: Microbial Strains and Culture Conditionsmentioning

confidence: 99%

Identification of specific binding sites for XYR1, a transcriptional activator of cellulolytic and xylanolytic genes in Trichoderma reesei

Furukawa

Shida

Kitagami

et al. 2009

Fungal Genetics and Biology

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118

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“…Recently, Kosalkova et al (2012) reported that casein or its biopolymers significantly increased the secretion of extracellular proteins up to 40-to 80-fold in A. awamori. Similarly Kawamori et al (1986) also tested l-sorbose for induction and production of CMCases in T. reesi, where avicel and glucose supplemented with 0.3% sorbose enhanced the CMCase activity by twofold. Cheese whey is generally used as a low-cost nitrogen source in large-scale enzyme productions from bacteria and fungi.…”

Section: Effect Of Additives On Cellulases Productionmentioning

confidence: 99%

Use of combined UV and chemical mutagenesis treatment of Aspergillus terreus D34 for hyper-production of cellulose-degrading enzymes and enzymatic hydrolysis of mild-alkali pretreated rice straw

Kumar

Parikh

Singh

et al. 2015

Bioresour. Bioprocess.

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Background: Microbial production of cellulose-degrading enzymes could be significantly improved using traditional mutagenesis treatment. Development of high-titre cellulase producing mutants drastically reduces the costs involved in cellulase production and downstream processing in commercial-scale enzyme production. Here, we have evaluated the efficacy of different Aspergillus terreus D34 mutants for hyper-production of improved cellulase enzymes utilizing locally available lignocellulosic biomass residues as growth substrates in solid state fermentation conditions. Further, enzymatic hydrolysis of mild-alkali pre-treated rice straw was performed using the improved cellulases.Results: A 4.9-fold higher β-glucosidase activity was obtained from ethyl methyl sulphonate (EMS) treated mutant strain (EMS2) when grown on mixed rice straw/sugarcane bagasse (RSBG) biomass growth substrate. Similarly with the EMS2 mutant and BG-grown culture extract a 1.1-fold higher xylanase activity was observed. Irrespective of the growth substrates and the mutant strains, the maximum cellulase (FPase, carboxymethyl cellulase, avicelase, β-glucosidase) and xylanase activities (U mL −1 ) were 2.34, 39.8, 2.46, 19.9 and 655, respectively. Further, external supplementation of 20% bovine serum albumin (BSA), 3% tween 80 and 20% polyethylene glycol (PEG) 6000 to the crude enzyme extract increased the FPase activity nearly 4.0-, 2.8-and 2.2-fold. Addition of 0.05% sodium benzoate marginally increased the stability of cellulase enzyme and retained more than 60% of the initial activity after 96 h incubation at 37°C. While at 4°C, no loss in enzyme activity was observed even after prolonged incubation period (up to 90 days). Further, maximum reducing sugars of 0.842 g g −1 at a rate of 0.25 mM g −1 h −1 at 10% biomass loading of mild-alkali pretreated rice straw was produced using the BG-grown culture extract of EMS2 mutant strain. Conclusion:The extracellular protein production and corresponding cellulase activities of A. terreus D34 were significantly enhanced after combined UV and chemical mutagenesis treatments. In the present study, besides accelerating the rate of cellulase production, we have also demonstrated production of high reducing sugars by enzymatic saccharification of pretreated lignocellulosic biomass using hyper-produced cellulase enzymes. Due to high enzyme activity of the cellulase enzymes produced from the mutant strains, the volume of enzyme loadings in enzymatic hydrolysis could be reduced up to 7-fold. These studies clearly show the potential of the developed hyper-cellulase producing mutants in decreasing the overall process economics of cellulosic ethanol technology.

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Induction and production of cellulases by l-sorbose in Trichoderma reesei

Cited by 49 publications

References 12 publications

Identification of Major Facilitator Transporters Involved in Cellulase Production during Lactose Culture ofTrichoderma reeseiPC-3-7

Identification of Major Facilitator Transporters Involved in Cellulase Production during Lactose Culture ofTrichoderma reeseiPC-3-7

Identification of specific binding sites for XYR1, a transcriptional activator of cellulolytic and xylanolytic genes in Trichoderma reesei

Use of combined UV and chemical mutagenesis treatment of Aspergillus terreus D34 for hyper-production of cellulose-degrading enzymes and enzymatic hydrolysis of mild-alkali pretreated rice straw

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