Induction based fluidics (IBF) for droplet‐based mass spectrometric analysis of oligonucleotides

Ross, Robert L; Sauter, Andrew D.; Limbach, Patrick A.

doi:10.1002/jms.3636

Cited by 5 publications

(3 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…94 Ross et al applied induction based fluidics (IBF) to inject charged nanodroplets directly into the inlet of a mass spectrometer for the analysis of nanomolar concentrations of oligonucleotides. 95 Forbes et al acoustically actuated droplet ejection combined with extractive ESI for the rapid detection of inorganic compounds. 96 Interlayer spray ionization for analysis of small volumes of liquid (<2 μL) with no pretreatment was proposed by Chen and co-workers.…”

Section: ■ Emerging Developments Related To Newer Ionization Technolo...mentioning

confidence: 99%

Advances in Ionization for Mass Spectrometry

Peacock¹,

Zhang

Trimpin

2016

Anal. Chem.

View full text Add to dashboard Cite

Section: ■ Emerging Developments Related To Newer Ionization Technolo...mentioning

confidence: 99%

Advances in Ionization for Mass Spectrometry

Peacock¹,

Zhang

Trimpin

2016

Anal. Chem.

View full text Add to dashboard Cite

“…Recently we investigated droplet-based sample introduction, using induction based fluidics (IBF), as a means of generating ESI-like data from oligonucleotides. 73 It appears that droplet-based sample introduction may retain the benefits of ESI, while reducing sampling bias for oligoribonucleotide mixtures. A different alternative has been the use of plasmabased ionization sources for the detection of oligoribonucleo-tides.…”

Section: New Ionization Methodsmentioning

confidence: 99%

Mass spectrometry of modified RNAs: recent developments

Wetzel

Limbach

2016

Analyst

Self Cite

View full text Add to dashboard Cite

A common feature of ribonucleic acids (RNAs) is that they can undergo a variety of chemical modifications. As nearly all of these chemical modifications result in an increase in the mass of the canonical nucleoside, mass spectrometry has long been a powerful approach for identifying and characterizing modified RNAs. Over the past several years, significant advances have been made in method development and software for interpreting tandem mass spectra resulting in approaches that can yield qualitative and quantitative information on RNA modifications, often at the level of sequence specificity. We discuss these advances along with instrumentation developments that have increased our ability to extract such information from relatively complex biological samples. With the increasing interest in how these modifications impact the epitranscriptome, mass spectrometry will continue to play an important role in bioanalytical investigations revolving around RNA.

show abstract

“…55 Negative ion ESI mass spectra of the samples were recorded by a Thermo Scientific LCQ Fleet ion trap mass spectrometry (San Jose, CA/Sangon Biotech Co. Beijing). 56 The obtained MS spectra were analyzed by ProMass software (an automated biomolecular deconvolution software), 57,58 and the deconvolution result was singly charged in positive mode.…”

Section: ■ Introductionmentioning

confidence: 99%

Investigation of Na⁺ and K⁺ Competitively Binding with a G-Quadruplex and Discovery of a Stable K⁺–Na⁺-Quadruplex

Zhou

et al. 2019

J. Phys. Chem. B

View full text Add to dashboard Cite

DNA G-quadruplex (G4) could adopt multiple conformations, and the exact conformation is related to the presence of cations. However, the fact that cations with various concentrations could competitively bind with G4 is rarely investigated, which greatly limits the potential applications of G4-based sensors. Here, with PW17 (a G4-forming DNA sequence) as an example, Na+ and K+ with different concentrations competitively binding with PW17 are clarified by circular dichroism spectroscopy and electrospray ionization mass spectroscopy. Although Na+ could induce PW17 switching to unstable and antiparallel Na+-stabilized PW17 (2Na+-PW17) (C Na+ = 5–70 mM) and further to stable and hybrid 2Na+-PW17 (C Na+ = 70–800 mM), K+ (C K+ = 0.1–10 mM) could replace Na+ in 2Na+-PW17 with 2Na+-PW17 transforming into K+-stabilized PW17 (2K+-PW17). Moreover, the replacing ability strictly relied on C K+ and C Na+ . In the switching process, a stable intermediate including a K+ and an Na+ in one G4 (K+–Na+-PW17) is firstly detected. Importantly, the stable K+–Na+-PW17 is detected at 0.5 mM K+ and 140 mM Na+. Based on the facts, the interferences of Na+ with the performance of PW17-based K+ sensors are investigated. With the stable K+–Na+-PW17 as a sensing probe and protoporphyrin IX (PPIX) as a G4 fluorescent read-out probe, a linear relationship between C K+ (500 nM–10 mM) and PPIX fluorescence is observed, which provides a fluorescence assay for detecting K+ with the co-existing 140 mM Na+. This study exhibits clear evidence of Na+ and K+ competitively binding with G4 and finds a novel and stable K+–Na+-PW17, which provides a clue to further explore G4 functions in Na+-contained samples.

show abstract

Induction based fluidics (IBF) for droplet‐based mass spectrometric analysis of oligonucleotides

Cited by 5 publications

References 19 publications

Advances in Ionization for Mass Spectrometry

Advances in Ionization for Mass Spectrometry

Mass spectrometry of modified RNAs: recent developments

Investigation of Na⁺ and K⁺ Competitively Binding with a G-Quadruplex and Discovery of a Stable K⁺–Na⁺-Quadruplex

Contact Info

Product

Resources

About

Induction based fluidics (IBF) for droplet‐based mass spectrometric analysis of oligonucleotides

Cited by 5 publications

References 19 publications

Advances in Ionization for Mass Spectrometry

Advances in Ionization for Mass Spectrometry

Mass spectrometry of modified RNAs: recent developments

Investigation of Na+ and K+ Competitively Binding with a G-Quadruplex and Discovery of a Stable K+–Na+-Quadruplex

Contact Info

Product

Resources

About

Investigation of Na⁺ and K⁺ Competitively Binding with a G-Quadruplex and Discovery of a Stable K⁺–Na⁺-Quadruplex