Induction by Cyanide, Dinitrophenol, and Trinitrophenol of Growth Inhibition and of Recovery from Far-Ultraviolet Killing in Escherichia coli B: Relationship to Photoprotection

Lakchaura, B. D.; Jagger, John

doi:10.2307/3573420

Cited by 20 publications

(14 citation statements)

References 14 publications

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“…Figure 1 shows that maximum photoprotection (PP) is obtained by 3 X 105 ergs mm-' of near u.v., which also kills about 25% of cells unirradiated by far U.V. In E. coli B, maximum PP can also be obtained by such a dose, and this also results in about 15-20% killing of the control population [4].…”

Section: Resultsmentioning

confidence: 97%

“…The mechanism of PP involves growth inhibition by near U.V. [3,4]. Growth inhibition of uur+rec-and uvr-rec+ cells is shown in Fig.…”

Section: Resultsmentioning

confidence: 98%

“…Growth inhibition caused by near U.V. was determined in a Bonet-Maury Biospectrophotometer, as earlier described [4]. Log-phase washed and starved cells (approximately 5 X 107/ml) were added to 6.5 ml of pre-warmed nutrient broth in a cylindrical glass cuvette (27-mm path length).…”

Section: Wmentioning

confidence: 99%

“…This phenomenon has been termed photoprotection (PP) (for review, see [ 1,2]). The mechanism proposed for PP involves temporary inhibition of normal growth [3,4], which extends the period in which existing cellular dark-repair systems may operate.…”

mentioning

confidence: 99%

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PHOTOPROTECTION FROM KILLING IN ULTRAVIOLET‐SENSITIVE Escherichia coli K‐12 MUTANTS: INVOLVEMENT OF EXCISION‐RESYNTHESIS REPAIR*

Lakchaura

1972

Photochem & Photobiology

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Section: Resultsmentioning

confidence: 97%

“…The mechanism of PP involves growth inhibition by near U.V. [3,4]. Growth inhibition of uur+rec-and uvr-rec+ cells is shown in Fig.…”

Section: Resultsmentioning

confidence: 98%

Section: Wmentioning

confidence: 99%

mentioning

confidence: 99%

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PHOTOPROTECTION FROM KILLING IN ULTRAVIOLET‐SENSITIVE Escherichia coli K‐12 MUTANTS: INVOLVEMENT OF EXCISION‐RESYNTHESIS REPAIR*

Lakchaura

1972

Photochem & Photobiology

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“…2 20 h)]. Lakchaura and Jagger (1972) showed that the kinetics and final extent of LHR in E. coli B can be stimulated by addition of glucose to the post-irradiation holding buffer; moreover, they found LHR to be inhibited by 2,4-dinitrophenol. Similar results were reported by for LHR in yeast.…”

Section: Comparison Of the Biological Response Of E Coli B/r Under Cmentioning

confidence: 99%

REPAIR OF UV DAMAGE IN ESCHERICHIA COLI UNDER NON‐GROWTH CONDITIONS

Tang¹,

Patrick²

1977

Photochem & Photobiology

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Abstract. –A large difference in survival occurs between buffered suspensions of E. coli irradiated with UV radiation at a low fluence rate and those irradiated at a high fluence rate. For sufficiently large fluences, the extent of this fluence rate dependent recovery (FRR) is about two orders of magnitude greater than that which can be brought about by liquid holding recovery (LHR) following high fluence rate irradiation in most of the E. coli strains studied. LHR and FRR occur in excision resynthesis repair proficient (ERR+) but not ERR‐ strains of E. coli, although its observation can be masked in strains with complete repair potential upon subsequent growth on nutrient plates. Accumulation of DNA strand interruptions and excision of cyclobutyl dipyrimidine occur during LHR and FRR but are more extensive for the latter. Our data suggest mat events beyond incision and excision occur during LHR and FRR, but differences in the extent of ERR during LHR and FRR cannot account for the difference in cell survival between these two phenomena.

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Optimization of near ultraviolet irradiation conditions for isolation of relaxed mutants of Escherichia coli

Riesenberg

Lang

1984

J of Basic Microbiology

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We describe optimized conditions for isolation of relaxed mutants of bacferia with 4-thiouridinecontaining tRNAs. The results presented here imply that besides the knowledge of the action spectra for near UV-induced growth inhibitlion of stringent and relaxed cells the fluence -fluence rate dependence of growth inhibition is an essential factor for optimizing the enrichment of relaxed mutants. We investigated systematically the dependence of growth inhibition of both stringent and relaxed strains of E. coli on the wavelength A, on the fluence F and on the fluence rate For eeveral years we have been studying the regulation of growth of the myceliumforming bacterium Streptomyces hygroscopicus (BERGTER 1978, BERGTER and RIESENBERG 1982. After amino acid starvation S . hygroscopicus accumulates guanosine-5'-diphosphate-3'-diphosphate (ppGpp) as it W~S first found for E. coli (CASHEL and GALLANT 1969) and subsequently for other bacterial species as well. Derivatives of stringent strains impaired in ppGpp synthesis are known as relaxed mutants. Comparative studies with stringent and relaxed strains of E . coli improved considerably the knowledge about regulation of growth. Accordingly, relaxed mutants of other bacteria like Bacillus subtilis (SWANTON and EDLW 1972), Salmonella typhimurium ( STEPHENS et al. 1975) and Klebsiella pneumoniae (RIESENBERG and K~R I 1981) were isolated. But, isolation of a relaxed mutant of a Streptomyces strain has not yet been reported. This is probably due to the fact that the methods developed for isolation of relaxed mutants of unicellular bacteria cannot be applied to mycelium-forming procaryotes (RIESENBERG and BERGTER 1984). The lack of such a mutant prompted us to develop a new strategy for the isolation of relaxed Streptomyces mutants by combining the turbidostat technique and near UV irradiation ( RIESENBERG et al. 1983). This seemed to be the method of choice. The chemostat technique cannot be used to isolate relaxed mutants because in mixed cultures relaxed cells are overgrown by stringent cells (RIESENBERG and BERGTER 1984).Near UV irradiation (300-400 nm) is known to induce a temporary inhibition of growth of E . coli (JAGGER et al. 1964) by photochemical crosslinking of 4-thiouridine in position 8 with cytidine in position 13 in 4-thiouridine-containing tRNAs ( THOMAS and FAVRE 1975and 1980, RAMABHADRAN et al. 1976. The photochemical alteration of tRNA causes a loss of its binding capacity to amino acids ( YANIV et al. 1971). Thus, E" = 48 kJ . m-2 and

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Induction by Cyanide, Dinitrophenol, and Trinitrophenol of Growth Inhibition and of Recovery from Far-Ultraviolet Killing in Escherichia coli B: Relationship to Photoprotection

Cited by 20 publications

References 14 publications

PHOTOPROTECTION FROM KILLING IN ULTRAVIOLET‐SENSITIVE Escherichia coli K‐12 MUTANTS: INVOLVEMENT OF EXCISION‐RESYNTHESIS REPAIR*

PHOTOPROTECTION FROM KILLING IN ULTRAVIOLET‐SENSITIVE Escherichia coli K‐12 MUTANTS: INVOLVEMENT OF EXCISION‐RESYNTHESIS REPAIR*

REPAIR OF UV DAMAGE IN ESCHERICHIA COLI UNDER NON‐GROWTH CONDITIONS

Optimization of near ultraviolet irradiation conditions for isolation of relaxed mutants of Escherichia coli

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