“…Nonsensitized mice were injected with a culture supernatant of the parental myeloma cell line. Both the sensitized and nonsensitized mice were challenged on days 1, 2, 3, and 8 under anesthesia with escain (Mylan, Osaka, Japan) with 1% OVA (grade V; Sigma-Aldrich, St. Louis, MO) in a volume of 20 ml by intratracheal administration as reported (26,27). Additionally, the mice sensitized with OE-1 on days 0, 1, 2, and 7 were challenged on days 1, 2, and 3 with 1% OVA, and then with saline on day 8 (OE-1-sensitized nonchallenged group).…”