Induction of a wheat β-1,3-glucanase gene during the defense response to<i>Bipolaris sorokiniana</i>

Aggarwal, Rashmi; Purwar, Shalini; Kharbikar, Lalit L.; Gupta, Suman

doi:10.1556/aphyt.46.2011.1.5

Cited by 17 publications

(6 citation statements)

References 21 publications

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“…Aggarwal et al [45] quantified the levels of glucanase expression over time in leaves of 12 wheat genotypes inoculated with Bipolaris sorokiniana and found that some genotypes were resistant to the fungus; seven genes were identified in the response to the fungus, among them the Glucanase I and Glucanase II genes. The Glucanase II gene was significantly upregulated in resistant genotypes 16 and 24 h after inoculation; its expression levels were increased up to 7-fold compared with the control.…”

Section: Resultsmentioning

confidence: 99%

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

et al. 2019

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There are several phytosanitary problems that have been causing serious damage to the Capsicum crops, including anthracnose. Upon attack by certain pathogens, various protein molecules are produced, which are known as proteins related to pathogenesis (PR proteins), including antimicrobial peptides such as protease inhibitors, defensins and lipid transfer proteins (LTPs). The objective of this work is to identify antimicrobial proteins and/or peptides of two genotypes from Capsicum annuum fruits infected with Colletotrichum gloeosporioides. The fungus was inoculated into Capsicum fruits by the deposition of a spore suspension (106 conidia ml−1), and after 24 and 48 h intervals, the fruits were removed from the humid chamber and subjected to a protein extraction process. Protein analysis of the extracts was performed by tricine gel electrophoresis and Western blotting. The distinctive bands between genotypes in the electrophoresis profiles were subjected to mass spectrometry sequencing. Trypsin inhibition assays, reverse zymographic detection of protease inhibition and β-1,3-glucanase activity assays were also performed and extracts were also tested for their ability to inhibit the growth of C. gloeosporioides fungi ‘in vitro’. There were several low molecular weight proteins in all treated samples, and some treatments in which antimicrobial peptides such as defensin, lipid transfer protein (LTP) and protease inhibitor have been identified. It was shown that the green fruits are more responsive to infection, showing the production of antimicrobial peptides in response to injury and inoculation of the fungus, what did not occur in ripe fruits under any treatment.

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Section: Resultsmentioning

confidence: 99%

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

et al. 2019

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“…β-1,3-glucanases play a significant role in defending the plant by degrading the cell wall of fungal pathogens [ 46 ]. In wheat, a higher expression of β-1,3-glucanase was observed in the resistant genotype in response to spot blotch disease as compared to the susceptible genotype [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

Deciphering the Host–Pathogen Interactome of the Wheat–Common Bunt System: A Step towards Enhanced Resilience in Next Generation Wheat

Kataria

Kaundal

2022

IJMS

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Common bunt, caused by two fungal species, Tilletia caries and Tilletia laevis, is one of the most potentially destructive diseases of wheat. Despite the availability of synthetic chemicals against the disease, organic agriculture relies greatly on resistant cultivars. Using two computational approaches—interolog and domain-based methods—a total of approximately 58 M and 56 M probable PPIs were predicted in T. aestivum–T. caries and T. aestivum–T. laevis interactomes, respectively. We also identified 648 and 575 effectors in the interactions from T. caries and T. laevis, respectively. The major host hubs belonged to the serine/threonine protein kinase, hsp70, and mitogen-activated protein kinase families, which are actively involved in plant immune signaling during stress conditions. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the host proteins revealed significant GO terms (O-methyltransferase activity, regulation of response to stimulus, and plastid envelope) and pathways (NF-kappa B signaling and the MAPK signaling pathway) related to plant defense against pathogens. Subcellular localization suggested that most of the pathogen proteins target the host in the plastid. Furthermore, a comparison between unique T. caries and T. laevis proteins was carried out. We also identified novel host candidates that are resistant to disease. Additionally, the host proteins that serve as transcription factors were also predicted.

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“…To examine further whether IK726 could induce resistance against B. sorokiniana , dot blots of the defence response genes PR‐1a, β ‐1,3‐glucanase (PR‐2) and chitinase (PR‐3) were performed. These genes were selected because PR proteins have previously been shown to be involved in the defence against B. sorokiniana in cereals . However, none of the genes showed elevated expression when comparing plants treated with IK726 + B. sorokiniana with plants treated with B. sorokiniana alone.…”

Section: Discussionmentioning

confidence: 99%

Clonostachys roseareduces spot blotch in barley by inhibiting prepenetration growth and sporulation ofBipolaris sorokinianawithout inducing resistance

2016

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Induction of a wheat β-1,3-glucanase gene during the defense response toBipolaris sorokiniana

Cited by 17 publications

References 21 publications

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

Deciphering the Host–Pathogen Interactome of the Wheat–Common Bunt System: A Step towards Enhanced Resilience in Next Generation Wheat

Clonostachys roseareduces spot blotch in barley by inhibiting prepenetration growth and sporulation ofBipolaris sorokinianawithout inducing resistance

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