Induction of adenocarcinoma from hamster pancreatic islet cells treated with N-nitrosobis(2-oxopropyl)amine in vitro

Schmied, Bruno M.; Liu, G.; Moyer, Mary Pat; Hernberg, I. S B; Sanger, Warren G.; Batra, Surinder K.; Pour, Parviz M.

doi:10.1093/carcin/20.2.317

Cited by 31 publications

(20 citation statements)

References 30 publications

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“…7,10 The sizes of the treated and untreated islets varied between 0.12 and 0.32 mm without significant difference between the two groups. After the islets were transferred into the flasks, all islets attached to the bottom of the flasks within a week and a large number of epithelial cells radiated from the islet cores into the surrounding area as reported.…”

Section: Resultsmentioning

confidence: 94%

“…After the islets were transferred into the flasks, all islets attached to the bottom of the flasks within a week and a large number of epithelial cells radiated from the islet cores into the surrounding area as reported. 7,10 These epithelial cells were gradually replaced by monomorphic undifferentiated cells. At 11 weeks and later, contrary to the monomorphic appearance of the MS7N cells, the MS7B cells became pleomorphic, with abundant eosinophilic cytoplasm and a few hyperchromatic nuclei surrounded by fine granules.…”

Section: Resultsmentioning

confidence: 99%

“…Although in humans most cancer types, including pleomorphic giant cell tumors, are thought to derive from ductal cells, in the hamster model many cancers originate from within islets, 1-3 most probably from stem cells. 10 In contrast to tumors arising from ducts (intraductal carcinomas), which remain within the ductal boundary for a long time before invasion, tumors developing within islets (intrainsular cancers) are invasive from their inception. 10 We and others have shown that in vitro treatment of hamster ductal cells with pancreatic carcinogens leads to malignant cell transformation and adenocarcinoma in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…10 In contrast to tumors arising from ducts (intraductal carcinomas), which remain within the ductal boundary for a long time before invasion, tumors developing within islets (intrainsular cancers) are invasive from their inception. 10 We and others have shown that in vitro treatment of hamster ductal cells with pancreatic carcinogens leads to malignant cell transformation and adenocarcinoma in vivo. 5,6 No giant cell carcinomas have ever been produced.…”

Section: Discussionmentioning

confidence: 99%

“…We have identified these cells in culture and have shown their response to BOP. 7,10 The reactivity of giant cells with NSE (a marker for neuroendocrine differentiation) and ␣1-antitrypsin, which has been shown to be expressed in human cancer cells assumed to derive from pancreatic stem cells, such as pancreatoblastoma and solid cystic tumors, [25][26][27] also points to their stem cell derivation.…”

Section: Discussionmentioning

confidence: 99%

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In Vitro Induction of Giant Cell Tumors from Cultured Hamster Islets Treated with N-Nitrosobis(2-Oxopropyl)amine

Matsuzaki

Schmied

Ulrich

et al. 2000

The American Journal of Pathology

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It is generally believed that over 90% of pancreatic cancers originate from ductal/ductular cells. Studies of the hamster pancreatic cancer model, which in biological and morphological aspects mimics human tumors, [1][2][3] however, have shown that pancreatic ductal adenocarcinoma originates not only from ductal/ductular cells but primarily from within islets, most probably from precursor (stem) cells.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

In Vitro Induction of Giant Cell Tumors from Cultured Hamster Islets Treated with N-Nitrosobis(2-Oxopropyl)amine

Matsuzaki

Schmied

Ulrich

et al. 2000

The American Journal of Pathology

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Diabetes Secondary to Acquired Disease to the Pancreas

Prato¹,

Coppelli²,

Tiengo³

2004

International Textbook of Diabetes Mellitus

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Secondary diabetes is a condition of hyperglycemia which develops after the destruction of the pancreatic tissue by an acquired disease with subsequent impairment of exocrine and endocrine pancreatic functions. In general, any pathologic process of the pancreatic acinar tissue may results in an insult to its endocrine component, leading to some degree of hormone insufficiency. This condition was first described in 1788 by Sir Thomas Cawley, but it was soon appreciated that hyperglycemia can ensues acute and chronic pancreatitis, partial and total pancreatectomy, neoplasia as well as systemic processes involving the pancreas, such as hemochromatosis and cystic fibrosis. The Expert Committee on the Diagnosis and Classification of Diabetes Mellitus of the American Diabetes Association has identified these forms of diabetes as “diabetes secondary to diseases of the exocrine pancreas.” The new diagnostic criteria for diabetes introduced by the ADA and WHO Expert Committees are likely to influence the prevalence of diabetes in patients with diseases of the exocrine pancreas, but direct assessment using the new criteria is still too scanty. When the 1979 NDDG and 1985 WHO criteria are used, it can be calculated that diabetes secondary to disease of the exocrine pancreas represents, approximately, 0.5% of all cases of diabetes mellitus. Its frequency is likely to be twice as high in populations with heavy alcohol consumption. In the tropics, the prevalence of diabetes secondary to fibrocalculous pancreatitis can be as high as 10–20%. At variance with the selective B‐cell destruction occurring in type 1 diabetes, pancreatic diabetes is characterized by concomitant destruction of all endocrine cells of the islet of Langherans. The dual insulin–glucagon deficit is responsible for many of the metabolic and clinical manifestation of this form of diabetes, including lower risk for ketoacidosis and more frequent and severe hypoglycemic episodes. Since the late 1950s, the general idea has been that secondary diabetes was not associated with vascular complications. However, with the improved life expectancy of these patients during the past few years, a similar prevalence of retinopathy and microalbuminuria has been described in pancreatic and type 1 diabetes mellitus. The knowledge of the hormonal, metabolic, and clinical features of these forms of diabetes is essential in defining the most appropriate therapeutic strategies.

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Clinical Assessment of Pancreatic Cancer: Is there a Chance for Early Diagnosis?

Pour¹

2004

Clinical Pancreatology

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Induction of adenocarcinoma from hamster pancreatic islet cells treated with N-nitrosobis(2-oxopropyl)amine in vitro

Cited by 31 publications

References 30 publications

In Vitro Induction of Giant Cell Tumors from Cultured Hamster Islets Treated with N-Nitrosobis(2-Oxopropyl)amine

In Vitro Induction of Giant Cell Tumors from Cultured Hamster Islets Treated with N-Nitrosobis(2-Oxopropyl)amine

Diabetes Secondary to Acquired Disease to the Pancreas

Clinical Assessment of Pancreatic Cancer: Is there a Chance for Early Diagnosis?

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