Induction of an Antibody Response in Cultures of Human Peripheral Blood Lymphocytes

Luzzati, Alma L.

doi:10.1016/b978-0-12-442702-0.50019-4

Cited by 16 publications

(26 citation statements)

References 11 publications

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“…609.002), as previously described [27], then layered over FicollHypaque (Pharmacia, Uppsala, Sweden) and centrifuged (400 g) at 208C for 40 min. Cells at the interface were harvested, washed and suspended in tissue culture medium.…”

Section: Cell Preparationmentioning

confidence: 99%

“…The method previously described in detail for the sheep erythrocyte (SRC) antigen [28,29] Serva, Heidelberg, Germany; mol. wt 6000), 8% human serum (heatinactivated and SRC-absorbed), and antibiotics.…”

Section: Induction Of Antigen-specific Antibody Responsementioning

confidence: 99%

“…Assay for specific PFC Cells producing specific anti-SRC antibody were visualized and counted with a slide modification [27] of the haemolysis-in-gel method. Three serial dilutions from each sample were tested in duplicate.…”

Section: Induction Of Antigen-specific Antibody Responsementioning

confidence: 99%

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Interferon-gamma (IFN-γ) can counteract the in vitro inhibitory effect of an HIV p24 immunosuppressive heptapeptide

Luzzati

Boirivant

Giacomini

et al. 1996

Clinical and Experimental Immunology

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SUMMARYPrevious work from our laboratory demonstrated that a synthetic heptapeptide (Ch7), corresponding to a conserved sequence of HIV core protein p24 (aa 232-238), was able to specifically abrogate antigeninduced responses in cultures of normal human peripheral blood lymphocytes (PBL). In the present study we show that Ch7 did not inhibit the induction of IFN-°-secreting cells nor the accumulation of IFN-°mRNA in antigen-stimulated cultures. However, delayed addition of recombinant human IFN-°t o Ch7-suppressed cultures was able to restore fully the capacity to mount an antigen-specific antibody response. Thus, although the Ch7 immunosuppressive effect may not be directly related to a decreased production of IFN-°, an increased level of this cytokine is certainly able to counteract the negative effect of the peptide.

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Section: Cell Preparationmentioning

confidence: 99%

Section: Induction Of Antigen-specific Antibody Responsementioning

confidence: 99%

See 1 more Smart Citation

Interferon-gamma (IFN-γ) can counteract the in vitro inhibitory effect of an HIV p24 immunosuppressive heptapeptide

Luzzati

Boirivant

Giacomini

et al. 1996

Clinical and Experimental Immunology

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show abstract

“…The method previously described in detail for the sheep red cell (SRC) antigen [9,10] was followed. Briefly, cells were suspended at 7 × 10 6 /ml in Iscove's modified Dulbecco's medium (IMDM; Sigma Chemical Co., St Louis, MO, USA) supplemented with 5 × 10 ¹5 M 2-mercaptoethanol (2-ME), 4% polyethylene glycol (PEG; Serva, Heidelberg, Germany; 6000 MW), 8% human serum (heat inactivated and SRC absorbed) and antibiotics.…”

Section: Methodsmentioning

confidence: 99%

Possible Role of the Plasminogen Receptor as a Site of Interaction of the Human Immunodeficiency Virus p24 Immunosuppressive Heptapeptide Ch7 with the Host Immune System

Giacomini

Chersi²,

Giordani

et al. 2000

Scand J Immunol

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Previous work from our laboratory demonstrated that a synthetic heptapeptide (Ch7: RGSDIAG), corresponding to a conserved sequence of human immunodeficiency virus (HIV) core protein p24 (amino acids 232-238), was able to specifically abrogate antigen-induced responses in cultures of normal human peripheral blood mononuclear cells (PBMC), probably acting at the level of monocytes. The Ch7 peptide displays sequence homology to human plasminogen. In the present report we show that a compound (6-aminoexanoic acid), known to prevent plasminogen binding to monocyte-like cells, greatly reduced the immunosuppressive capacity of Ch7. We suggest that the plasminogen receptor may represent a target structure on human monocytes for the immunosuppressive p24 sequence.

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“…However, an early waning of humoral immunity was observed even in children who received highly efficacious acellular vaccines [ 8, 191. Although serum antibody titres decay rapidly following antigen exposure, it is possible that memory B and T cells persist after vaccination or exposure and this may result in the capacity of circulating lymphocytes to mount a specific recall antibody response in vitro to B. pertussis antigens. To test this hypothesis, and based on previous experience in the induction and maintenance passed through a synthetic wool column (Coop, Basel, Switzerland) to remove strongly adhering suppressor cells, as described previously [22], then layered over Ficoll-Hypaque (Pharmacia, Uppsala, Sweden) and centrifuged (400 g) at 20°C for 40min. Cells at the interface were harvested, washed and suspended in tissue-culture medium.…”

Section: Introductionmentioning

confidence: 99%

Induction of a specific antibody response to Bordetella pertussis antigens in cultures of human peripheral blood mononuclear cells

Giacomini¹,

Urbani

Ausiello

et al. 1999

Journal of Medical Microbiology

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The role of specific antibodies in protective immunity to Bordetella pertussis has not yet been clearly defined. In the present work, the induction of a specific antibody response to B. pertussis in cultures of human peripheral blood mononuclear cells (PBMC) was investigated, on the assumption that the capacity of circulating lymphocytes to mount a specific response in vitro may provide a useful parameter for the evaluation of protective immunity. When PBMC from normal adult donors were cultured with a heatinactivated B. pertussis whole-cell suspension, cells secreting antibodies to pertussis toxin, pertactin and filamentous haemagglutinin were generated consistently. The antibody response peaked between days 7 and 11 of culture and the antibodies produced were exclusively of the IgM class.

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Induction of an Antibody Response in Cultures of Human Peripheral Blood Lymphocytes

Cited by 16 publications

References 11 publications

Interferon-gamma (IFN-γ) can counteract the in vitro inhibitory effect of an HIV p24 immunosuppressive heptapeptide

Interferon-gamma (IFN-γ) can counteract the in vitro inhibitory effect of an HIV p24 immunosuppressive heptapeptide

Possible Role of the Plasminogen Receptor as a Site of Interaction of the Human Immunodeficiency Virus p24 Immunosuppressive Heptapeptide Ch7 with the Host Immune System

Induction of a specific antibody response to Bordetella pertussis antigens in cultures of human peripheral blood mononuclear cells

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