Induction of androgenetic development of the brook charr (Salvelinus fontinalis)×Arctic charr (Salvelinus alpinus) hybrids in eggs derived from the parental species

Ocalewicz, Konrad; Kuźmiński, H.; Pomianowski, Konrad; Dobosz, Stefan

doi:10.1016/j.repbio.2013.03.002

Cited by 25 publications

(21 citation statements)

References 24 publications

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“…Moreover, it has been reported that mutated lbx genes may provoke spine deformations during early fish ontogeny as well as during adolescence when females are more susceptible to the disease [11]. The described results suggest that individuals with scoliosis among DH fish may appear also later, after the larval stage of development, which in turn would explain the observations of fish with spinal deformities among one-year-old DH salmonids [34, 35]. …”

Section: Discussionmentioning

confidence: 69%

High Rate of Deformed Larvae among Gynogenetic Brown Trout (Salmo trutta m. fario) Doubled Haploids

Jagiełło

Zalewski

Dobosz

et al. 2017

BioMed Research International

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Mitotic gynogenesis results in the production of fully homozygous individuals in a single generation. Since inbred fish were found to exhibit an increased frequency of body deformations that may affect their survival, the main focus of this research was to evaluate the ratio of individuals with spinal deformities among gynogenetic doubled haploids (DHs) brown trout as compared to nonmanipulated heterozygous individuals. Gynogenetic development was induced by the activation of brown trout eggs by UV-irradiated homologous and heterologous (rainbow trout) spermatozoa. The subsequent exposure of the activated eggs to the high hydrostatic pressure disturbed the first cleavage in gynogenetic zygotes and enabled duplication of the maternal haploid set of chromosomes. The survival rate was significantly higher among gynogenetic brown trout hatched from eggs activated with the homologous UV-irradiated spermatozoa when compared to DHs hatched from eggs activated by the heterologous spermatozoa. More than 35% of the gynogenetic larvae exhibited body deformities, mostly lordosis and scoliosis. The percentage of malformed brown trout from the control group did not exceed 15%. The increased number of deformed larvae among DHs brown trout suggested rather a genetic background of the disease related to the fish spine deformities; however, both genetic and environmental factors were discussed as a cause of such conditions in fish.

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Section: Discussionmentioning

confidence: 69%

High Rate of Deformed Larvae among Gynogenetic Brown Trout (Salmo trutta m. fario) Doubled Haploids

Jagiełło

Zalewski

Dobosz

et al. 2017

BioMed Research International

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“…Somatic metaphase plates were prepared as described by Ocalewicz et al. () with small modifications. Briefly, fishes were injected with 0.3% colchicine solution and sacrificed after 90 min.…”

Section: Methodsmentioning

confidence: 99%

Activation of the Albino Sterlet Acipenser ruthenus Eggs by UV‐Irradiated Bester Hybrid Spermatozoa to Provide Gynogenetic Progeny

Fopp‐Bayat

Ocalewicz

2015

Reprod Domestic Animals

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Meiotic gynogenesis was induced in the albino form of sterlet Acipenser ruthenus by activation of eggs with UV-irradiated bester (Huso huso x Acipenser ruthenus) spermatozoa followed by inhibition of the second meiotic division performed by a heat shock. Obtained putative gynogenetic progeny were all albinos. The genetic verification based on three microsatellite DNA markers confirmed the only maternal inheritance of the progeny from the gynogenetic experimental groups. Cytogenetic analysis proved the gynogenetic sterlets were diploids. Application of the albino phenotype together with the molecular and the cytogenetic diagnostic approaches enabled to evaluate the efficiency of the spermatozoa irradiation and application of the heat shock to restore diploid state in the gynogenetic zygotes.

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“…Metaphase chromosomes of the androgenetic hatched fish were prepared from the cephalic kidney cells according to previously described method (Ocalewicz et al. ).…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, nineteen androgenetic haploid eyed-stage embryos developing in the brook charr eggs (n = 18) and the hybrids charr egg (n = 1) were cytogenetically studied (Experiment 2). Metaphase chromosomes of the androgenetic hatched fish were prepared from the cephalic kidney cells according to previously described method (Ocalewicz et al 2013a).…”

Section: Cytogenetic Analysis Of Embryosmentioning

confidence: 99%

Use of Eggs Derived From the Interspecific Charr Hybrids to Induce Androgenetic Development of the Brook Charr (Salvelinus fontinalis Mitchill 1814)

Michalik

Dobosz

Wójcik

et al. 2013

Reprod Domestic Animals

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Although, brook charr (Salvelinus fontinalis Mitchill 1814) and Arctic charr (Salvelinus alpinus Linnaeus 1758) are able to cross and give fertile offspring, their androgenetic nucleocytoplasmic hybrids are not viable. To overcome incompatibility between the egg cytoplasm of one charr species and the sperm nucleus of another charr species, application of F1 interspecific hybrids as egg donors for the purpose of androgenesis has been proposed. Here, androgenetic development of the brook charr was successfully induced in the brook charr eggs and the eggs derived from the reciprocal brook charr × Arctic charr F1 hybrids. A working androgenesis protocol included inactivation of the maternal nuclear DNA achieved by irradiation of the eggs with 420 Gy of X-rays, insemination of such treated eggs with the haploid sperm cells and exposition of the haploid androgenetic zygotes to the high hydrostatic pressure shock (51.711 MPa for 4 min) applied 420 min after insemination. Androgenetic larvae that hatched from the brook charr and the hybrid eggs were shown to be homozygous brook charr individuals. Androgenetic individuals exhibited 84 chromosomes and 100 chromosome arms (FN), values characteristic for the brook charr diploid cells. Strategy hybridize first than induce androgenesis should be tested in order to provide androgenetic offspring in other salmonids that are able to cross and produce fertile offspring.

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Induction of androgenetic development of the brook charr (Salvelinus fontinalis)×Arctic charr (Salvelinus alpinus) hybrids in eggs derived from the parental species

Cited by 25 publications

References 24 publications

High Rate of Deformed Larvae among Gynogenetic Brown Trout (Salmo trutta m. fario) Doubled Haploids

High Rate of Deformed Larvae among Gynogenetic Brown Trout (Salmo trutta m. fario) Doubled Haploids

Activation of the Albino Sterlet Acipenser ruthenus Eggs by UV‐Irradiated Bester Hybrid Spermatozoa to Provide Gynogenetic Progeny

Use of Eggs Derived From the Interspecific Charr Hybrids to Induce Androgenetic Development of the Brook Charr (Salvelinus fontinalis Mitchill 1814)

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