Induction of apoptosis and ganoderic acid biosynthesis by cAMP signaling in Ganoderma lucidum

You, Bang‐Jau; Tien, Ni; Lee, Miin-Huey; Bao, Bo-Ying; Wu, Yih Shyuan; Hu, Tsung-Chi; Lee, Hong‐Zin

doi:10.1038/s41598-017-00281-x

Cited by 32 publications

(15 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It seems unlikely that such inhibition could be connected with the suppression of acetylcholinesterase activity. Alternatively, a previous report showed the ability of the active ingredients in GL to modify the cAMP signaling [52]. However, GTs-mediated inhibition of I h in GH 3 cells could not be reversed by further addition of SQ-22536 (10 µM), an inhibitor of adenylate cyclase, (data not shown), suggesting that such an inhibitory action is apparently unlinked to the GTs effectiveness in cAMP signaling.…”

Section: Resultsmentioning

confidence: 87%

Evidence for Effective Inhibitory Actions on Hyperpolarization-Activated Cation Current Caused by Ganoderma Triterpenoids, the Main Active Constitutents of Ganoderma Spores

Chang

Gao

et al. 2019

Molecules

View full text Add to dashboard Cite

The triterpenoid fraction of Ganoderma (Ganoderma triterpenoids, GTs) has been increasingly demonstrated to provide effective antioxidant, neuroprotective or cardioprotective activities. However, whether GTs is capable of perturbing the transmembrane ionic currents existing in electrically excitable cells is not thoroughly investigated. In this study, an attempt was made to study whether GTs could modify hyperpolarization-activated cation currents (Ih) in pituitary tumor (GH3) cells and in HL-1 atrial cardiomyocytes. In whole-cell current recordings, the addition of GTs produced a dose-dependent reduction in the amplitude of Ih in GH3 cells with an IC50 value of 11.7 µg/mL, in combination with a lengthening in activation time constant of the current. GTs (10 µg/mL) also caused a conceivable shift in the steady-state activation curve of Ih along the voltage axis to a more negative potential by approximately 11 mV. Subsequent addition of neither 8-cyclopentyl-1,3-dipropylxanthine nor 8-(p-sulfophenyl)theophylline, still in the presence of GTs, could attenuate GTs-mediated inhibition of Ih. In current-clamp voltage recordings, GTs diminished the firing frequency of spontaneous action potentials in GH3 cells, and it also decreased the amplitude of sag potential in response to hyperpolarizing current stimuli. In murine HL-1 cardiomyocytes, the GTs addition also suppressed the amplitude of Ih effectively. In DPCPX (1 µM)-treated HL-1 cells, the inhibitory effect of GTs on Ih remained efficacious. Collectively, the inhibition of Ih caused by GTs is independent of its possible binding to adenosine receptors and it might have profound influence in electrical behaviors of different types of electrically excitable cells (e.g., pituitary and heart cells) if similar in vitro or in vivo findings occur.

show abstract

Section: Resultsmentioning

confidence: 87%

Evidence for Effective Inhibitory Actions on Hyperpolarization-Activated Cation Current Caused by Ganoderma Triterpenoids, the Main Active Constitutents of Ganoderma Spores

Chang

Gao

et al. 2019

Molecules

View full text Add to dashboard Cite

show abstract

“…(Ganodermataceae, Agaricomycetes) emerges as an important source of bioactive compounds that can be used in the diet as supplementation, aiming to improve health [16,17]. Known as the "elixir of life" or "mushroom of immortality," officially used as a dietary supplement, this mushroom presents popular indications for "improving memory," "retarding senility," among other purposes [18][19][20][21]. Bioactive compounds such as triterpenes, lectins, and polysaccharides have been described in their composition [21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Ganoderma lucidumAmeliorates Neurobehavioral Changes and Oxidative Stress Induced by Ethanol Binge Drinking

Nascimento

Luz

Silva

et al. 2020

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Ganoderma lucidum, mushroom used for centuries by Asian peoples as food supplement, has been shown interesting biological activities, including over the Central Nervous System. Besides, these mushroom bioactive compounds present antioxidant and anti-inflammatory activities. On the side, binge drinking paradigm consists of ethanol exposure that reflects the usual consumption of adolescents, which elicits deleterious effects, determined by high ethanol consumption, in a short period. In this study, we investigated whether the Aqueous Extract of G. lucidum (AEGl) reduces the behavioral disorders induced by alcohol. Male (n=30) and female Wistar rats (n=40), seventy-two days old, were used for behavioral/biochemical and oral toxicity test, respectively. Animals were exposed to 5 binges (beginning at 35 days old) of ethanol (3 g/kg/day) or distilled water. Twenty-four hours after the last binge administration, animals received AEGl (100 mg/kg/day) or distilled water for three consecutive days. After treatment protocol, open field, elevated plus maze, forced swim, and step-down inhibitory avoidance tests were performed. Oxidative stress parameters were measured to evaluate the REDOX balance. Our results demonstrated that AEGl elicited the recovery of spontaneous horizontal exploration capacity, anxiogenic- and depressive-profile, as well as short-term memory damage induced by binge-ethanol exposure. The behavioral effects of the extract were associated to the reequilibrium of the animals’ REDOX balance. Thus, AEGl, a medicinal mushroom, ameliorates behavioral alteration on a model of motor, cognitive and psychiatric-like disorders induced by binge drinking paradigm and emerges as a useful tool as a food supplement in the management of disorders of alcoholic origin.

show abstract

“…Moreover, melanin can also be produced from catechol, which is one of the degradation products of SA [38,59]. SA treatments have been found to induce production of reactive oxygen species (ROS) and cause cell apoptosis in G. lucidum [60][61][62]. Similarly, at later stage of infection by G. boninense, ROS will be produced as a result of lignin degradation, which ultimately may results in formation of phenoxyl radicals [2,63].…”

Section: Metabolites Production By G Boninense In Defence Against Salicylic Acid Stressmentioning

confidence: 99%

Growth modulation and metabolic responses of Ganoderma boninense to salicylic acid stress

Ong¹,

Ahmad

Goh³

et al. 2021

PLoS ONE

View full text Add to dashboard Cite

Various phenolic compounds have been screened against Ganoderma boninense, the fungal pathogen causing basal stem rot in oil palms. In this study, we focused on the effects of salicylic acid (SA) on the growth of three G. boninense isolates with different levels of aggressiveness. In addition, study on untargeted metabolite profiling was conducted to investigate the metabolomic responses of G. boninense towards salicylic acid. The inhibitory effects of salicylic acid were both concentration- (P < 0.001) and isolate-dependent (P < 0.001). Also, growth-promoting effect was observed in one of the isolates at low concentrations of salicylic acid where it could have been utilized by G. boninense as a source of carbon and energy. Besides, adaptation towards salicylic acid treatment was evident in this study for all isolates, particularly at high concentrations. In other words, inhibitory effect of salicylic acid treatment on the fungal growth declined over time. In terms of metabolomics response to salicylic acid treatment, G. boninense produced several metabolites such as coumarin and azatyrosine, which suggests that salicylic acid modulates the developmental switch in G. boninense towards the defense mode for its survival. Furthermore, the liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) analysis showed that the growth of G. boninense on potato dextrose agar involved at least four metabolic pathways: amino acid metabolism, lipid pathway, tryptophan pathway and phenylalanine pathway. Overall, there were 17 metabolites that contributed to treatment separation, each with P<0.005. The release of several antimicrobial metabolites such as eudistomin I may enhance G. boninense’s competitiveness against other microorganisms during colonisation. Our findings demonstrated the metabolic versatility of G. boninense towards changes in carbon sources and stress factors. G. boninense was shown to be capable of responding to salicylic acid treatment by switching its developmental stage.

show abstract

Induction of apoptosis and ganoderic acid biosynthesis by cAMP signaling in Ganoderma lucidum

Cited by 32 publications

References 58 publications

Evidence for Effective Inhibitory Actions on Hyperpolarization-Activated Cation Current Caused by Ganoderma Triterpenoids, the Main Active Constitutents of Ganoderma Spores

Evidence for Effective Inhibitory Actions on Hyperpolarization-Activated Cation Current Caused by Ganoderma Triterpenoids, the Main Active Constitutents of Ganoderma Spores

Ganoderma lucidumAmeliorates Neurobehavioral Changes and Oxidative Stress Induced by Ethanol Binge Drinking

Growth modulation and metabolic responses of Ganoderma boninense to salicylic acid stress

Contact Info

Product

Resources

About