Induction of Apoptosis by Cold Atmospheric Pressure Plasma for Oral Squamous Cell Carcinoma Cells

Ramireddy, Latha; Lai, Chih Ho; Low, Bih Show; Li, Chuan; Hsieh, J.H.; Lee, Jyh-Wei; Wu, Hui

doi:10.1615/plasmamed.2019028601

Cited by 5 publications

(9 citation statements)

References 6 publications

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“…The selective toxicity of plasma treatment in vitro was demonstrated since human malignant keratinocytes (SCC) were significantly more sensitive to plasma treatment compared to human non-malignant (HaCaT) keratinocytes. This is in line with previous reports, showing a cytotoxic action and self-amplification of mitochondrial ROS production, following treatment in various human SCC cell lines, including FaDu, SNU1041, OSC19, SNU800, and HN9 [ 47 , 71 , 72 ]. Another study reported the involvement of catalytic iron in eliciting ferroptosis in plasma-treated human and mouse SCC lines, such as SAS, Ca9-22, HSC-2, HSC-3, HSC-4, Sa3, and Ho-1-u-1 [ 73 ].…”

Section: Discussionsupporting

confidence: 93%

Plasma Treatment Limits Cutaneous Squamous Cell Carcinoma Development In Vitro and In Vivo

Pasqual-Melo

Nascimento

Sanches

et al. 2020

Cancers

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Cutaneous squamous cell carcinoma (SCC) is the most prevalent cancer worldwide, increasing the cost of healthcare services and with a high rate of morbidity. Its etiology is linked to chronic ultraviolet (UV) exposure that leads to malignant transformation of keratinocytes. Invasive growth and metastasis are severe consequences of this process. Therapy-resistant and highly aggressive SCC is frequently fatal, exemplifying the need for novel treatment strategies. Cold physical plasma is a partially ionized gas, expelling therapeutic doses of reactive oxygen and nitrogen species that were investigated for their anticancer capacity against SCC in vitro and SCC-like lesions in vivo. Using the kINPen argon plasma jet, a selective growth-reducing action of plasma treatment was identified in two SCC cell lines in 2D and 3D cultures. In vivo, plasma treatment limited the progression of UVB-induced SSC-like skin lesions and dermal degeneration without compromising lesional or non-lesional skin. In lesional tissue, this was associated with a decrease in cell proliferation and the antioxidant transcription factor Nrf2 following plasma treatment, while catalase expression was increased. Analysis of skin adjacent to the lesions and determination of global antioxidant parameters confirmed the local but not systemic action of the plasma anticancer therapy in vivo.

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Section: Discussionsupporting

confidence: 93%

Plasma Treatment Limits Cutaneous Squamous Cell Carcinoma Development In Vitro and In Vivo

Pasqual-Melo

Nascimento

Sanches

et al. 2020

Cancers

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“…Kang et al showed the ROS-mediated inactivation of plasma-treated head and neck cancer cells could be rescued via the addition of antioxidants, which abolished plasma-mediated cytotoxicity through the reduction of apoptosis, levels of intracellular ROS, mitochondrial superoxide, and the loss of mitochondrial membrane potential (MMP) [32]. Besides the initiation of cell death mechanisms, ROS cause the general elevation of oxidative stress within cancer cells [26]. These ROS or secondary ROS may be able to alter cellular structures, such as DNA and RNA, and proteins could be damaged, potentially leading to autophagy and induction of the DNA-damage response.…”

Section: Hnscc and Oxidative Stressmentioning

confidence: 99%

“…Most of these experimental in vitro studies used the oral squamous cell carcinoma cell line SCC-15, as well as SCC-25, and highlighted their plasma-induced inactivation due to the reduction of cell growth, induction of apoptosis, and secondary DNA damage, especially through plasma-derived ROS [19,25,28,[34][35][36][37][38]. Other HNSCC cell lines were also tested, showing one or more of the tumor-toxic plasma-associated characteristics [6,19,22,24,[26][27][28][29][30][31][32][33]37]. In addition, HNSCC has been shown to be more prone to physical plasma treatment than non-malignant cells since the viability of the latter did not significantly decrease after plasma application.…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

“…The additional intracellular oxidative stress that is generated by plasma-derived ROS leads to further imbalances in the cellular redox state and the loss of the MMP. This results in cell damage of squamous carcinoma cells after a plasma-mediated increase of intracellular ROS levels [26,28,30,32]. Furthermore, physical-plasma-induced ROS selectively enhances the iron-dependent lipid peroxidation and mitochondrial superoxide formation in HNSCC cells since they harbor more catalytic Fe(II) than non-malignant cells.…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

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Medical Gas Plasma Treatment in Head and Neck Cancer—Challenges and Opportunities

et al. 2020

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Despite progress in oncotherapy, cancer is still among the deadliest diseases in the Western world, emphasizing the demand for novel treatment avenues. Cold physical plasma has shown antitumor activity in experimental models of, e.g., glioblastoma, colorectal cancer, breast carcinoma, osteosarcoma, bladder cancer, and melanoma in vitro and in vivo. In addition, clinical case reports have demonstrated that physical plasma reduces the microbial contamination of severely infected tumor wounds and ulcerations, as is often seen with head and neck cancer patients. These antimicrobial and antitumor killing properties make physical plasma a promising tool for the treatment of head and neck cancer. Moreover, this type of cancer is easily accessible from the outside, facilitating the possibility of several rounds of topical gas plasma treatment of the same patient. Gas plasma treatment of head and neck cancer induces diverse effects via the deposition of a plethora of reactive oxygen and nitrogen species that mediate redox-biochemical processes, and ultimately, selective cancer cell death. The main advantage of medical gas plasma treatment in oncology is the lack of adverse events and significant side effects compared to other treatment modalities, such as surgical approaches, chemotherapeutics, and radiotherapy, making plasma treatment an attractive strategy for the adjuvant and palliative treatment of head and neck cancer. This review outlines the state of the art and progress in investigating physical plasma as a novel treatment modality in the therapy of head and neck squamous cell carcinoma.

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“…Among these cancer types, the research presenting the beneficial effects of CAP on head and neck cancer only account for 3.28%, and research on the anti-cancer effect of CAP on OSCC is rare. Ramireddy et al reported that helium plasma jet treatment on OSCC causes apoptotic cell death due to the increase of oxidative stress in tumor cells [ 20 ]. In the case of Lee et al, it has been reported that the treatment of OSCC with nitrogen-based plasma jets induced apoptotic cell death, and they also proposed the increased intracellular level of reactive oxygen species (ROS) as a reason for this phenomenon [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Cancer Activity of the Combinational Treatment of Noozone Cold Plasma with p-FAK Antibody-Conjugated Gold Nanoparticles in OSCC Xenograft Mice

Choi¹,

Gu²,

Park³

et al. 2022

Biomedicines

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Oral squamous cell cancer (OSCC) is the most common type of oral cancer (about 80–90% of cases) and various research is being done to cure the disease. This paper aims to verify whether treatment with no-ozone cold plasma (NCP), which is designed for safe usage of the plasma on oral cavities, in combination with gold nanoparticles conjugated with p-FAK antibody (p-FAK/GNP) can trigger the selective and instant killing of SCC-25 cells both in vitro and in vivo. When SCC25 and HaCaT cells are exposed to p-FAK/GNP+NCP, the instant cell death was observed only in SCC25 cells. Such p-FAK/GNP+NCP-mediated cell death was observed only when NCP was directly treated on SCC25 harboring p-FAK/GNP. During NCP treatment, the removal of charged particles from NCP using grounded electric mesh radically decreased the p-FAK/GNP+NCP-mediated cell death. This p-FAK/GNP+NCP-mediated selective cell death of OSCC was also observed in mice xenograft models using SCC25 cells. The mere treatment of p-FAK/GNP and NCP on the xenograft tumor slowly decreased the size of the tumor, and only about 50% of the tumor remained at the end of the experiment. On the other hand, 1 week of p-FAK/GNP+NCP treatment was enough to reduce half of the tumor size, and most of tumor tissue had vanished at the end. An analysis of isolated tissues showed that in the case of individual treatment with p-FAK/GNP or NCP, the cancer cell population was reduced due to apoptotic cell death. However, in the case of p-FAK/GNP+NCP, apoptotic cell death was unobserved, and most tissues were composed of collagen. Thus, this paper suggests the possibility of p-FAK/GNP+NCP as a new method for treating OSCC.

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Induction of Apoptosis by Cold Atmospheric Pressure Plasma for Oral Squamous Cell Carcinoma Cells

Cited by 5 publications

References 6 publications

Plasma Treatment Limits Cutaneous Squamous Cell Carcinoma Development In Vitro and In Vivo

Plasma Treatment Limits Cutaneous Squamous Cell Carcinoma Development In Vitro and In Vivo

Medical Gas Plasma Treatment in Head and Neck Cancer—Challenges and Opportunities

Anti-Cancer Activity of the Combinational Treatment of Noozone Cold Plasma with p-FAK Antibody-Conjugated Gold Nanoparticles in OSCC Xenograft Mice

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