Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma

Aghbali, Amirala; Hosseini, Sepideh Vosough; Delazar, Abbas; Gharavi, Nader Kalbasi; Shahneh, Fatemeh Zare; Orangi, Mona; Bandehagh, Ali; Baradaran, Behzad

doi:10.17305/bjbms.2013.2360

Cited by 31 publications

(26 citation statements)

References 28 publications

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“…Apoptosis is characterized by morphological changes for instance, cell shrinkage, membrane blebbing, chromatine condensation, DNA fragmentation and loosing organelles position in cytoplasm (Aghbali et al 2013;Galluzzi et al 2007). It has been shown that some plants such as saffron, curcumin and Ginger suppressed carcinogenic progress through induction of apoptosis (Bakhshi et al 2008Bathaie et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…In brief, MCF-7 cells were treated with alcoholic extract of Berberis (1.5 and 3 mg/ml) for 48 h. DNA samples were isolated after cell lysis according to previous studies (Aghbali et al 2013;Samarghandian and Shabestari 2013). The samples were analyzed by electrophoresis on 1.5 % agarose gel in TAE buffer containing 0.5 lg/ml ethidium bromide.…”

Section: Dna Fragmentation Assaymentioning

confidence: 99%

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The antioxidant and chemical properties of Berberis vulgaris and its cytotoxic effect on human breast carcinoma cells

2015

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In this study we evaluated the biological activity of alcoholic and aqueous extracts from the fruit of Berberis vulgaris. The total antioxidant capacity of Berberis was characterized by FRAP, DPPH, Folin-Ciocalteu while the anthocyanins content was measured by pH differential method. Cell viability and apoptotic property were determined by MTT and DNA fragmentation assays, respectively. Alcoholic extract of Berberis was richer in antioxidants and anthocyanins compared to aqueous extract. Although both extracts significantly inhibited proliferation of breast cancer cells (MCF-7); these changes were not observed in normal human breast epithelial cells (MCF10-A). The alcoholic extract was more effective in inducing apoptosis as detected by DNA fragmentation in treated cancer cells. Our results suggest that Berberis has potent antioxidant properties and cytotoxic effects that can induce apoptosis. Therefore, Berberis can potentially be exploited for the development of therapeutics to fight against human breast cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Dna Fragmentation Assaymentioning

confidence: 99%

The antioxidant and chemical properties of Berberis vulgaris and its cytotoxic effect on human breast carcinoma cells

2015

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“…), apoptotic effects in tumour cells (Aghbali et al . , Yen et al . ) and effects on bone metabolism (Ishikawa et al .…”

Section: Introductionmentioning

confidence: 99%

“…Proanthocyanidins (PACs) have been investigated in the areas of medical and nutritional health for various biological effects, such as antioxidant capacity (Houde et al 2006, Katsuda et al 2015, apoptotic effects in tumour cells (Aghbali et al 2013, Yen et al 2015 and effects on bone metabolism (Ishikawa et al 2005, Yahara et al 2005. In dentistry, the antibacterial capacity of PACs has been assessed in endodontics (Ghonmode et al 2013) and periodontics (Houde et al 2006, Mageshwaran et al 2012) and more recently as a crosslinker , 2010 in Restorative Dentistry.…”

Section: Introductionmentioning

confidence: 99%

Direct and transdentinal biostimulatory effects of grape seed extract rich in proanthocyanidin on pulp cells

et al. 2018

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Aim To evaluate the potential biostimulatory effects of grape seed extract (GSE) on a primary culture of human pulp cells. Methodology Human molars were used to obtain the primary pulp cell culture and 0.5‐mm dentine discs. For GSE direct exposure, dose–response (0.0065–6.5%) and time response (1–60 min of contact) were examined. For transdentinal exposure, 0.65% of GSE was tested for 24 h. Cellular metabolism, nitric oxide and collagen production, and cell morphology alterations were assessed at periods of 24 and 72 h. After cell differentiation and direct exposure to GSE, the total protein production (TP), alkaline phosphatase activity (ALP) and formation of mineralization nodules (MN) were assessed. The results were analysed by parametric tests or non‐parametric tests (α = 0.05). Results The lower concentration of GSE tested (0.0065%) was associated with an increase in cellular metabolism, a reduction in the production of nitric oxide and an increase in extracellular matrix synthesis (collagen). Distinct behaviours were observed for the different concentrations, without a reduction of cellular metabolism >10% compared with the control, either when applied directly or transdentinally. SEM revealed no significant change in cell morphology, except for the positive control (H2O2). There was no difference in TP, ALP or MN between the control group and the group exposed to GSE. Conclusions Treatment with grape seed extract, even at the highest concentration and longest period, caused neither direct nor transdentinal cytotoxic effects on human pulp cells. Grape seed extract components may play a biostimulatory role and protect dental pulp cells when in direct contact.

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“…Oral squamous cell carcinoma (OSCC) is among the most prevalent oral cancers, the risk of which is increasing in individuals under 40 years old, particularly in developed countries (Aghbali et al, 2013). The proposed risk factors include micronutrient deficiencies, chronic trauma, poor oral hygiene, and some specific microorganisms like viruses (Lauritano et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Anti-Cancer Effects of Traditional Medicinal Herbs on Oral Squamous Cell Carcinoma

Nazhvani

Sarafraz

Askari

et al. 2020

Asian Pac J Cancer Prev

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Backgrounds: Oral squamous cell carcinoma (OSCC) is among the most frequent oral cancers in individuals under 40. Documents have endorsed that a diet enriched with fruit and vegetables can banish the risk of developing major cancers. This study aimed to evaluate the effects of different concentrations of four medicinal herbs including saffron, ginger, cinnamon and curcumin on OSCC cell line. Methods: Having obtained the aqueous extract of the four herbs, they were administered on OSCC cell lines per se and in dual, triple, and quadruple combinations. Their effects were measured in different concentrations and in 24 and 48 hours by using MTT assay. Results: The minimum and maximum effective concentrations were respectively 108 and 217 mg/ml for curcumin with IC30 of 77mg/ml, 108 and 270 mg/ml for ginger with IC30 of 58 mg/ml, 2 and 10 mg/ml for saffron with IC30 of 1.9 mg/ml, and 5 and 40 mg/ml for cinnamon with IC30 of 3.3 mg/ml. The best effect of the combinations was seen for cinnamon-saffron after both 24 and 48 hours and the four herbs combination after 48 hours. Conclusion: Although all the four herbs were effective on OSCC cell line, the strongest extract was saffron, followed by cinnamon. Combination of cinnamon-saffron and combination of the four herbs showed maximum effects. These findings suggest that traditional medicinal herbs may potentially contribute to oral cancer treatment; providing new windows for the development of new therapeutic strategies for OSCC.

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Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma

Cited by 31 publications

References 28 publications

The antioxidant and chemical properties of Berberis vulgaris and its cytotoxic effect on human breast carcinoma cells

The antioxidant and chemical properties of Berberis vulgaris and its cytotoxic effect on human breast carcinoma cells

Direct and transdentinal biostimulatory effects of grape seed extract rich in proanthocyanidin on pulp cells

Anti-Cancer Effects of Traditional Medicinal Herbs on Oral Squamous Cell Carcinoma

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