Induction of apoptosis in A431 cells via ROS generation and p53-mediated pathway by chloroform fraction of Argemone mexicana (Pepaveraceae)

Attri, Shivani; Kaur, Prabhjot; Singh, Davinder; Kaur, Harneetpal; Rashid, Farhana; Kumar, Avinash; Singh, Balbir; Bedi, Neena; Arora, Saroj

doi:10.1007/s11356-021-16696-z

Cited by 7 publications

(5 citation statements)

References 66 publications

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“…The loading and entrapment effectiveness of the cubosomes is generally high in binary lipid matrices, as reported in previous findings ( Soni et al, 2017 ; Attri et al, 2022 ) Entrapment efficiency and drug loading of ER in cubosomes was determined by using HPLC method. % EE and % DL of the cubosomes can be logically considered to be the primary indicators of miscibility between the drug and copolymers ( Supplementary Figure S3 ).…”

Section: Resultssupporting

confidence: 67%

“…The cellular uptake studies were performed using acridine-orange (AO) and ethidium-bromide (EtBr) (AO/EtBr) staining with Fluorescence microscopy. The mechanism of cell death and alterations in nuclei during apoptosis was analyzed in EAC cells using AO/EtBr staining with method suggested by ( Attri et al, 2022 ). For 24 h, EAC cells (4×10 5 /well) were treated with ER, BLK-CUB and ER-CUB.…”

Section: Introductionmentioning

confidence: 99%

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Development and optimization of nanoparticles loaded with erucin, a dietary isothiocyanate isolated from Eruca sativa: Antioxidant and antiproliferative activities in ehrlich-ascites carcinoma cell line

Singh

Attri

et al. 2023

Front. Pharmacol.

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The study on Erucin (ER) has gained interest of nutraceutical and pharmaceutical industries because of its anti-cancer properties. Erucin is an isothiocyanate obtained from the seeds of Eruca sativa which possess certain drawbacks such as poor aqueous solubility and bioavailability. Therefore, the present study aimed at developing ER-cubosomes (CUB) by solvent evaporation technique followed by applying Central Composite Design to optimize ER loaded cubosomes. For this purpose, independent variables selected were Monoolein (MO) as lipid and Pluronic-84 (P-84) as a stabilizer whereas dependent variables were particle size, percentage of ER loading and percentage of its entrapment efficiency. The cubosomal nanocarriers exhibited particle size in the range of 26 nm, entrapment efficiency of 99.12 ± 0.04% and drug loading of 3.96 ± 0.0001%. Furthermore, to investigate the antioxidant potential, we checked the effect of ER and ER-CUB by DNA nicking assay, DDPH assay and Phosphomolybdate assay, and results showed significant improvement in antioxidant potential for ER-CUB than ER. Similarly, ER-CUB showed enhanced anticancer activity with a marked reduction in IC50 value than ER in MTT assay. These results suggested that ER-CUB produced notable escalation in antioxidant potential and enhanced anticancer activity than ER.

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Section: Resultssupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Development and optimization of nanoparticles loaded with erucin, a dietary isothiocyanate isolated from Eruca sativa: Antioxidant and antiproliferative activities in ehrlich-ascites carcinoma cell line

Singh

Attri

et al. 2023

Front. Pharmacol.

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“…The chloroform fraction of AM induces apoptosis through the regulation of apoptosis-related genes such as p53, Fas, PUMA, and APAF1. The presence of polyphenolic compounds contributes to its potency as an anti-proliferative agent (81).…”

Section: Anticancer Activity Of Ammentioning

confidence: 99%

Traditional uses, phytochemistry, pharmacological significance and toxicology of Argemone mexicana L. – A review approach

2024

IJAM

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Argemone Mexicana L. (AM, Papaveraceae) is found and native to tropical America. Many cultures have traditionally used this annual herb to treat a variety of ailments, including skin disorders, digestive problems, eye issues, and anti-dotes. Argemone Mexicana (AM), a plant with a long history of traditional use, has recently gained renewed attention from researchers due to its potential pharmacological benefits. The possibility that this plant could be used to develop new therapies has inspired further research. Information about AM was collected from scientific databases published between January 2000 and March 2022. These databases include Elsevier, PubMed, Web of Science, NOPR, Google Scholar, ResearchGate, Wiley, SpringerLink, and ACS publications. Numerous chemical constituents present in AM have been found to have medicinal properties, such as alkaloids, flavonoids, tannins, phenolic compounds, saponin, terpenoids, and cardiac glycosides. Various parts of the AM plant exhibit antimicrobial, wound healing, antioxidant, antimalarial, larvicidal, anthelmintic, antidiabetic, hepatoprotective, anticancer effects, and have been used to treat sexually transmitted diseases. However, the plant is also known to be toxic, and its use can lead to a number of adverse effects, including dermatitis, gastrointestinal disturbances, and neurological damage. This review critically evaluates recent developments in the traditional use, phytochemistry, pharmacology, and toxicology of AM in order to provide a scientific basis for reasonable utilization and further research.

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“…The generation of reactive oxygen species (ROS) is continuous during aerobic metabolism causing oxidative damage, mitochondrial disruption, dysfunction of the membrane, DNA disintegration, and ultimately ion leakage into intercellular space (Karimi and Noori 2022 ; Attri et al 2022 ). At moderate and low concentrations, ROS plays a significant signaling molecule role in mitogenesis, or as a host defense mechanism during infections (Ser et al 2016a , b ).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and chemoprotective potential of Streptomyces levis strain isolated from human gut

2023

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In the current study, Streptomyceslevis strain HFM-2 has been isolated from healthy human gut. Streptomyces sp. HFM-2 was identified based on the polyphasic approach that included cultural, morphological, chemotaxonomical, phylogenetic, physiological, and biochemical characteristics. 16S rRNA gene sequence of strain HFM-2 exhibited 100% similarity with Streptomyceslevis strain 15423 (T). The EtOAc extract of Streptomyceslevis strain HFM-2 showed potential antioxidant activity, along with 69.53 ± 0.19%, 64.76 ± 0.13%, and 84.82 ± 0.21% of scavenging activity for ABTS, DPPH, and superoxide radicals, respectively at 600 µg/mL. The IC50 values i.e. 50% scavenging activity for DPPH, ABTS, and superoxide radicals were achieved at 497.19, 388.13, and 268.79 (µg/mL), respectively. The extract's reducing power and total antioxidant capacity were determined to be 856.83 ± 0.76 and 860.06 ± 0.01 µg AAE/mg of dry extract, respectively. In addition, the EtOAc extract showed protection against DNA damage from oxidative stress caused by Fenton’s reagent, and cytotoxic activity against HeLa cervical cancer, Skin (431) cancer, Ehrlich-Lettre Ascites-E (EAC) carcinoma, and L929 normal cell lines. The IC50 values against HeLa, 431 skin, and EAC carcinoma cell lines were found to be 50.69, 84.07, and 164.91 µg/mL, respectively. The EtOAc extract showed no toxicity towards L929 normal cells. In addition, flow cytometric analysis exhibited reduced mitochondrial membrane potential (MMP), and enhanced levels of reactive oxygen species (ROS). The EtOAc extract was chemically analyzed using GCMS to determine the components executing its bioactivities.

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Induction of apoptosis in A431 cells via ROS generation and p53-mediated pathway by chloroform fraction of Argemone mexicana (Pepaveraceae)

Cited by 7 publications

References 66 publications

Development and optimization of nanoparticles loaded with erucin, a dietary isothiocyanate isolated from Eruca sativa: Antioxidant and antiproliferative activities in ehrlich-ascites carcinoma cell line

Development and optimization of nanoparticles loaded with erucin, a dietary isothiocyanate isolated from Eruca sativa: Antioxidant and antiproliferative activities in ehrlich-ascites carcinoma cell line

Traditional uses, phytochemistry, pharmacological significance and toxicology of Argemone mexicana L. – A review approach

Antioxidant and chemoprotective potential of Streptomyces levis strain isolated from human gut

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