Induction of apoptosis in HPV16 E7 transfected human keratinocyte by ALA-mediated photodynamic therapy

Miao, Fei; Lv, Ting; Zhang, Yunfeng; Huang, Zheng; Wang, Xiuli; Wang, Hongwei

doi:10.1016/j.pdpdt.2015.07.004

Cited by 11 publications

(5 citation statements)

References 38 publications

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“…An in vitro 2016 study revealed that PDT can induce apoptosis in human keratinocytes transfected with HPV16 E7 [33]. The current literature reports that the rate of HPV clearance after PDT in cervical precancerous lesions ranges from 53.4% to 94.4%.…”

Section: Discussionmentioning

confidence: 99%

“…We used a brush to collect the exfoliative epithelium cells of the vagina and cervix. The Cobas ® HPV test (Roche Molecular Diagnostics, Basel, Switzerland) was performed to detect 14 high-risk types of HPV DNA, including HPV 16,18,31,33,35,39,45,51,52,56,58,59, 66, and 68. ThinPrep fixative solution was used to collect pap smear samples, and an automated stainer was applied.…”

Section: Hr-hpv Test Cytological Examination and Pathological Biopsy ...mentioning

confidence: 99%

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Efficacy and Safety of 5-Aminolevulinic Acid Photodynamic Therapy for Treating Cervical and Vaginal Intraepithelial Neoplasia

Han,

Guo,

et al. 2024

Pharmaceutics

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Persistent HPV infections may cause cervical and vaginal intraepithelial neoplasia (CIN and VaIN). Traditional methods might destroy the structure and function of the cervix. 5-aminolevulinic acid photodynamic therapy (ALA-PDT) is a non-invasive targeted therapy. This study aims to evaluate the efficacy and safety of ALA-PDT for CIN and VaIN and the clearance of HPV. A retrospective study of 303 patients who confirmed CIN or VaIN and received ALA-PDT was conducted. All the patients were followed up at six and twelve months after treatment and then annually thereafter. The effect was evaluated through HPV genotyping, a cytology test, and colposcopy-directed biopsy if necessary. After ALA-PDT, the remission rates for CIN 2, CIN 3, VaIN 2, and VaIN 3 were 90.6%, 88.5%, 87.3%, and 77.8%. For CIN 1, the remission rate at the six-month follow-up was 93.1%. The total HPV clearance rates were 72.5% at the six-month follow-up and 85.7% at the 12-month follow-up. The most common adverse event was vaginal discharge. No severe adverse effect was observed. ALA-PDT is an effective and safe treatment for all grades of CIN and VaIN and is helpful in clearing HPV with minimal side effects. This treatment may not influence fertility and delivery.

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Section: Discussionmentioning

confidence: 99%

Section: Hr-hpv Test Cytological Examination and Pathological Biopsy ...mentioning

confidence: 99%

Efficacy and Safety of 5-Aminolevulinic Acid Photodynamic Therapy for Treating Cervical and Vaginal Intraepithelial Neoplasia

Han,

Guo,

et al. 2024

Pharmaceutics

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“…[17] According to studies, 5-ALA PDT can lower the amount of HPV viruses by preventing the expression of the E6 and E7 viruses. [18,19] In this clinical trial, patients underwent 2 PDTs at 1-week intervals as outpatient treatment and achieved favorable results with no serious adverse events. Over a long follow-up period, 76.47% of HR-HPV remission occurred in the ALA PDT group.…”

Section: Discussionmentioning

confidence: 99%

The effect of aminolevulinic acid hydrochloride topical powder photodynamic therapy versus nocardia rubra cell wall skeleton and observers group in the treatment of cervical low-grade squamous intraepithelial lesions with human papillomavirus infection: A non-randomized study, controlled pilot study

et al. 2023

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There are insufficient studies comparing the efficacy of aminolevulinic acid hydrochloride topical powder (ALA) photodynamic therapy (PDT) against Nocardia rubra cell wall skeleton (Nr-CWS) therapy in the treatment of cervical low-grade squamous intraepithelial lesion (LSIL) with human papillomavirus (HPV), especially for long-term efficacy. Patients with cervical LSIL and HPV infection were divided into 3 treatment groups based on their own choice. All patients had a follow-up test including HPV testing, cytology and colposcopy at 4 to 6 months and 12 months after the treatment. Among142 patients, patients received 51 ALA PDT and 41 patients received Nr-CWS. Another 50 patients who refused treatment were included in the Observers group. Four to six months or 12 months after treatment, there was significant difference between 3 groups in the clearance rate of HR-HPV infection and the complete remission (CR) rates of cervical LSIL; the CR rates of cervical LSIL in the ALA PDT group was significantly higher than the Nr-CWS group; but there was no significant difference between 2 groups in the clearance rate of HPV infection. The CR rates of cervical LSIL and the clearance rate of HPV infection in the ALA PDT group was significantly higher than the Observers group; the CR rates of cervical LSIL and the clearance rate of HPV infection in the Nr-CWS group was significantly higher than the Observers group; there was no significant difference in the recurrence rates in ALA PDT and Nr-CWS group after 12 months. Both of ALA PDT and Nr-CWS group had lower recurrence rate than the Observers group. The effect of ALA PDT is similar to Nr-CWS in the clearance rate of HR-HPV infection. Compared to the Nr-CWS group, the CR rates of cervical LSIL were considerably greater in the ALA PDT group. The effect of ALA PDT in the clearance rate of HPV infection and CR rates of cervical LSIL was significantly higher than the follow-up group; Both of ALA PDT and Nr-CWS group had lower recurrence rate than the Observers group. For cervical LSIL with HPV infection, ALA PDT is a very successful therapeutic method that is noninvasive.

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“…While high recurrence rate is one of the most common and intractable drawbacks of traditional treatments for genital warts, PDT has a significantly lower recurrence rate compared with CO 2 -LT, topical TAA, and other treatments. It has been reported that a photosensitizer could be taken up selectively by HPV-infected cells and induce apoptosis during PDT treatment [23]. Based on the fluorescence examination, it was recommended to apply the photosensitizer to not only the wart lesions but also to a surrounding 2 cm rim in clinical practice [12,24].…”

Section: Discussionmentioning

confidence: 99%

Topical photodynamic therapy for genital warts: Systematic review and meta‐analysis

Zhi

Wang

Zhang

et al. 2022

Transl Biophotonics

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High recurrence rate and adverse effects were common shortcomings in treatments for genital warts, but a number of clinical studies have found that photodynamic therapy (PDT) had some unique superiority compared to other treatments. This study aims to evaluate the efficacy and safety of PDT for genital warts in comparison to alternative treatments. Seven databases and http://clinicaltrials.gov were searched and seven randomized controlled trials were finally included. There was no significant difference in clearance rate of urethral lesions between the PDT group and the CO2 laser therapy (CO2‐LT group) (Odds ratio [OR] 0.676, 95% confidence interval [CI] 0.071–6.426, P = 0.734), but the overall clearance rate of all genital warts was slightly lower for PDT (OR 0.574, CI 0.335–0.985, P = 0.044). The recurrence rate after PDT was significantly lower than after CO2‐LT (OR 0.318, CI 0.220–0.460, P < 0.001), and adverse effects were also slighter after PDT (OR 0.015, CI 0.003–0.074, P < 0.001). In addition, a modification of parameters of light irradiation provides the potential to alleviate pain. This study shows that PDT is an effective treatment for genital warts with good tolerability and safety, especially for cavitary lesions. Some modified protocols probably have the potential to reduce adverse effects, but still need further investigation.

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Induction of apoptosis in HPV16 E7 transfected human keratinocyte by ALA-mediated photodynamic therapy

Cited by 11 publications

References 38 publications

Efficacy and Safety of 5-Aminolevulinic Acid Photodynamic Therapy for Treating Cervical and Vaginal Intraepithelial Neoplasia

Efficacy and Safety of 5-Aminolevulinic Acid Photodynamic Therapy for Treating Cervical and Vaginal Intraepithelial Neoplasia

The effect of aminolevulinic acid hydrochloride topical powder photodynamic therapy versus nocardia rubra cell wall skeleton and observers group in the treatment of cervical low-grade squamous intraepithelial lesions with human papillomavirus infection: A non-randomized study, controlled pilot study

Topical photodynamic therapy for genital warts: Systematic review and meta‐analysis

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