Induction of Apoptosis in Human Promyelocytic Leukemia HL60 Cells by an Extract From<i>Erythrina suberosa</i>Stem Bark

Agrawal, Sunil; Agrawal, Madhunika; Sharma, Parduman R.; Gupta, B. K.; Arora, Saroj; Saxena, Ajit Kumar

doi:10.1080/01635581.2011.573900

Cited by 24 publications

(12 citation statements)

References 42 publications

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“…Erythrina plant species are widely used in folk medicine to treat health conditions including agitation, insomnia, anxiety and inflammation (21,22). A previous study has revealed that an alcoholic extract of the stem bark of E. suberosa induces apoptosis in human promyelocytic leukemia HL-60 cells (23). The aim of the current study was to investigate the anticancer potential of the flavonoid wighteone isolated from the stem bark of E. suberosa, and to study HSP90 expression and its correlation with wighteone metabolite response in HER2-positive breast cancer cells.…”

Section: Introductionmentioning

confidence: 99%

HSP90 expression and its association with wighteone metabolite response in HER2-positive breast cancer cells

et al. 2016

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Abstract.It is well known that heat shock protein 90 (HSP90) overexpression is correlated with poor prognosis and chemo-resistance in human malignant cancers. At the same time, wighteone, or 6-prenyl-5,7,4'-trihydroxyisoflavone, a major isoflavone component of the ornamental tall tree Erythrina suberosa, has been demonstrated to exhibit a potent anti-proliferative effect on human leukemia HL-60 cancer cell lines. In this study, the effects of wighteone on the proliferation of HER2-positive breast cancer cells were investigated, and the action mechanism was explored. MCF-7 HER2-positive breast cancer cells were treated with various concentrations of wighteone. The growth inhibitory rate of the cells was calculated by MTT assay, apoptosis was detected by flow cytometry, and the expression level of HSP90 was assessed by western blot analysis. The addition of wighteone at concentrations ranging from 1-10 g/ml in the medium for 48 h had a marked inhibition on the proliferation of HER2-positive cancer cell lines. The growth inhibitory rates with 0.5, 2 or 8 mM wighteone were significantly higher compared with the control group. Apoptosis in the wighteone-treated cells was also significantly higher compared with the control group. The expression level of HSP90 in the wighteone group was significantly lower than that in the control group. Our findings demonstrated that wighteone effectively inhibited the proliferation of HER2-positive cancer cell lines, and this is considered to be the result of downregulating HSP90 receptor and downstream signaling.

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Section: Introductionmentioning

confidence: 99%

HSP90 expression and its association with wighteone metabolite response in HER2-positive breast cancer cells

et al. 2016

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“…[ 20 ] Through human promyelocytic leukemia HL 60 cells, it has been found that stem bark of E. suberosa has apoptosis-inducing effects. [ 21 ] From aqueous leaf extracts of E. suberosa , biosynthesized silver nanoparticles were obtained, which were found with potentially effective anticancer activities. [ 18 ] Most of these studies were focused on leaf and flower extract, whereas our study was focused on the bark, and hence the results were nonetheless compatible with previous studies.…”

Section: R Esults and Discussionmentioning

confidence: 99%

In vitro cytotoxic and antimicrobial activities of Erythrina suberosa (Roxb) bark

et al. 2020

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A BSTRACT Background: The study was focused on evaluating cytotoxic and antimicrobial activities of Erythrina suberosa (Roxb.) bark through in vitro pharmacological screening. Materials and Methods: The bark was extracted using different solvents, for example, dichloromethane, ethyl acetate, methanol, and aqueous for obtaining the organic fractions. These organic fractions were then evaluated for their cytotoxic and antimicrobial activity compared with the standard. Cefixime was used as the standard for antibacterial assay, whereas clotrimazole was used as the standard for antifungal activities. Bacterial strains used were Staphylococcus aureus and methicillin-resistant S. aureus (MRSA), whereas for antifungal activities Candida albicans , Candida parapsilosis , and Candida krusei strains were used. Results: The organic fractions obtained were evaluated for their cytotoxic and antimicrobial activities. In cytotoxic assay (Brine shrimp lethality assay), dichloromethane fraction was the most potent with LD 50 of 47.63, whereas aqueous, methanol, and ethyl acetate fractions showed LD 50 of 121.74, 422.2, and 201.96, respectively. Similarly, for antibacterial assay, dichloromethane fraction showed 32.2mm zone of inhibition against MRSA in comparison with standard cefixime (zone of inhibition, 30.5mm). A minimal zone of inhibition with crude saponins (13.1 and 12.2mm) was observed against C. albicans in comparison to standard (cefixime) with a zone of inhibition of 28.5mm. No prominent results were observed against C. parapsilosis and C. krusei strains. Conclusion: The study was based on the plant from Indo-Pak origin, and it has shown some prominent cytotoxic and antibacterial activities. Although the results of this study have provided a basic idea about the efficacy of plant extract, still more explanatory and high-scale studies can be beneficial for elaborating the cytotoxic and antimicrobial activities of this plant.

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“…Additionally, human cancer cell lines were investigated: HL60 (human promyelocytic leukemia) cells were tested with E. suberosa bark ethanolic extract [28]. Cell proliferation was inhibited in a dose-and time-dependent manner and the cell cycle was modulated by an increase in subG0/G1 cell phase.…”

Section: Pharmacological Propertiesmentioning

confidence: 99%

Erythrina suberosa: Ethnopharmacology, Phytochemistry and Biological Activities

et al. 2019

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Plants are a great and irreplaceable source of medicines, fuel, food, energy and even cosmetics. Since prehistory, humans have learned to use plants for survival, growth and proliferation and still today it relies on natural and cultivated vegetables for food and the source of novel compounds with pharmacological activity. Not only herbs and flowers, but also trees are used. Indeed, Erythrina suberosa Roxb. is a deciduous tree of the family Fabaceae, common in Southeast Asia. In India, E. suberosa is called the “corky coral tree” or simply the “Indian coral tree”, given its peculiar red-orange flowers that can flower throughout the year and its corky irregular bark covered by prickles. It is a plant commonly used as an ornamental tree, but it also holds ethnopharmacological and socioeconomic uses. This article explored phytobiological features of E. suberosa, analysing its taxonomy, examining its traditional and common uses and investigating its bioactive components and pharmacological properties.

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Induction of Apoptosis in Human Promyelocytic Leukemia HL60 Cells by an Extract FromErythrina suberosaStem Bark

Cited by 24 publications

References 42 publications

HSP90 expression and its association with wighteone metabolite response in HER2-positive breast cancer cells

HSP90 expression and its association with wighteone metabolite response in HER2-positive breast cancer cells

In vitro cytotoxic and antimicrobial activities of Erythrina suberosa (Roxb) bark

Erythrina suberosa: Ethnopharmacology, Phytochemistry and Biological Activities

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