Induction of ASAP (MAP9) contributes to p53 stabilization in response to DNA damage

Basbous, Jihane; Knani, Dora; Bonneaud, Nathalie; Giorgi, Dominique; Brondello, Jean-Marc; Rouquier, Sylvie

doi:10.4161/cc.20858

Cited by 14 publications

(13 citation statements)

References 48 publications

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“…MAP9 is phosphorylated by the mitotic kinases Aurora A (AURKA) [20] and Polo-like kinase 1 (PLK1) [21] to ensure bipolar spindle assembly and centrosome integrity. We have also shown that, in response to DNA damage, MAP9 interacts with and stabilizes the tumor-suppressor TP53 [22]. Furthermore, we recently demonstrated that a normal Map9 function is required for the MT network to allow the first steps of development to proceed [23].…”

Section: Introductionmentioning

confidence: 99%

Expression of the Microtubule-Associated Protein MAP9/ASAP and Its Partners AURKA and PLK1 in Colorectal and Breast Cancers

et al. 2014

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Background. Colorectal and breast cancers are among the most common cancers worldwide. They result from a conjugated deficiency of gene maintenance and cell cycle control. Objective. We investigate the expression of the microtubule-associated protein MAP9/ASAP and its two partners AURKA and PLK1 in colorectal tumors as well as in ductal breast cancers. Materials and Methods. 26 colorectal cancer samples and adjacent normal tissues and 77 ductal breast cancer samples from grade I to grade III were collected. Real-time quantitative PCR was used to analyse the expression of MAP9, AURKA, and PLK1. Results. Expression of MAP9 is downregulated in colorectal cancer compared to normal tissues (P > 10−3), whereas those of AURKA and PLK1 are upregulated (P > 10−4). In ductal breast cancer, we found a grade-dependent increase of AURKA expression (P > 10−3), while the variations of expression of MAP9 and PLK1 are not significant (P > 0.2). Conclusions. MAP9 downregulation is associated with colorectal malignancy and could be used as a disease marker and a new drug target, while AURKA and PLK1 are upregulated. In ductal breast cancer, AURKA overexpression is strongly associated with the tumor grade and is therefore of prognostic value for the progression of the disease.

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Section: Introductionmentioning

confidence: 99%

Expression of the Microtubule-Associated Protein MAP9/ASAP and Its Partners AURKA and PLK1 in Colorectal and Breast Cancers

et al. 2014

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“…New evidence demonstrates that MAP9 directly interacts with the N-terminus of p53 in response to DNA damage. In doing so, it helps to stabilize p53 and promote p53-dependent transcription (Basbous et al, 2012). Thus, MAP9 is a newly discovered DNA damage receptor component, and its mutation or dysregulation is closely related to tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

MAP9 single nucleotide polymorphism rs1058992 is a risk of EBV-associated gastric carcinoma in Chinese population

Liu¹,

Xiao²,

Wu³

et al. 2018

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Microtubule-associated protein 9 (MAP9) is a mitosis-associated protein involved in bipolar spindle assembly. Following DNA damage, MAP9 stabilizes p53 via p300 and MDM2 (mouse double minute-2 homolog). The dysregulation of MAP9 was considered to be associated with tumorigenesis. Single nucleotide polymorphisms (SNPs) in key genes governing mitosis may particularly increase susceptibility to gastric carcinoma (GC). Our study demonstrated that the CC homozygous genotype of SNP rs1058992 located in the MAP9 gene was significantly correlated with EBV-associated GC (EBVaGC) in a recessive genetic model (OR = 2.558, 95% CI = 1.306-5.010, P = 0.043), and the C allele frequency of rs1058992 also showed significant correlation with EBVaGC (OR = 1.904, 95% CI = 1.141-3.179, P = 0.013). These results suggest that the MAP9 rs1058992 polymorphism is associated with risk of EBVaGC. The conversion of lysine to arginine caused by rs1058992 may affect development of EBVaGC; however, further studies in larger populations are needed to fully elucidate its role in EBVaGC.

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“…MAP9 is phosphorylated and regulated by the mitotic kinases Aurora A and PLK1 (Eot-Houllier et al 2010;Venoux et al 2008). It has also been demonstrated that, in response to DNA damage, MAP9 can interact and stabilize p53 (Basbous et al 2012). In zebra fish, MAP9 is expressed during early embryo development and localizes to the mitotic spindle and centrosomes.…”

Section: Map9 Family Of Microtubule-associated Proteinsmentioning

confidence: 99%

Microtubule Organization and Microtubule-Associated Proteins (MAPs)

2016

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Dendrites have a unique microtubule organization. In vertebrates, dendritic microtubules are organized in antiparallel bundles, oriented with their plus ends either pointing away or toward the soma. The mixed microtubule arrays control intracellular trafficking and local signaling pathways, and are essential for dendrite development and function. The organization of microtubule arrays largely depends on the combined function of different microtubule regulatory factors or generally named microtubule-associated proteins (MAPs). Classical MAPs, also called structural MAPs, were identified more than 20 years ago based on their ability to bind to and copurify with microtubules. Most classical MAPs bind along the microtubule lattice and regulate microtubule polymerization, bundling, and stabilization. Recent evidences suggest that classical MAPs also guide motor protein transport, interact with the actin cytoskeleton, and act in various neuronal signaling networks. Here, we give an overview of microtubule organization in dendrites and the role of classical MAPs in dendrite development, dendritic spine formation, and synaptic plasticity. IntroductionMicrotubules (MTs) are cytoskeletal structures that play essential roles in all eukaryotic cells. MTs are important not only during cell division but also in non-dividing cells, where they are critical structures in numerous cellular processes such as cell motility, migration, differentiation, intracellular transport and organelle positioning. MTs are composed of two proteins, α-and β-tubulin, that form heterodimers and organize themselves in a head-to-tail manner. MTs are dynamic and they can rapidly switch between cycles of growth and shrinkage. This MT

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Induction of ASAP (MAP9) contributes to p53 stabilization in response to DNA damage

Cited by 14 publications

References 48 publications

Expression of the Microtubule-Associated Protein MAP9/ASAP and Its Partners AURKA and PLK1 in Colorectal and Breast Cancers

Expression of the Microtubule-Associated Protein MAP9/ASAP and Its Partners AURKA and PLK1 in Colorectal and Breast Cancers

MAP9 single nucleotide polymorphism rs1058992 is a risk of EBV-associated gastric carcinoma in Chinese population

Microtubule Organization and Microtubule-Associated Proteins (MAPs)

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