Induction of beta-lactamase production in Pseudomonas aeruginosa biofilm

Giwercman, Birgit; Jensen, Elsebeth Tvenstrup; Høiby, Niels; Kharazmi, Arsalan; Costerton, J. W.

doi:10.1128/aac.35.5.1008

Cited by 160 publications

(100 citation statements)

References 14 publications

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“…Limited diffusion can also protect biofilms from degradable antimicrobials. Indeed, P. aeruginosa produces AmpC ␤-lactamase, and it has been demonstrated that 2.5% of clinical isolates from CF patient sputa are totally derepressed, with a high basal level of enzyme production that can be increased further through ␤-lactam-mediated induction and ␤-lactamase accumulation in the biofilm matrix (49,50). In clinical samples, insertion sequences inactivating the ampD gene have been described for CF patients with constitutively high expression of ␤-lactamase (51).…”

Section: Biofilm Recalcitrance Is Multifactorialmentioning

confidence: 99%

Biofilm-Related Infections: Bridging the Gap between Clinical Management and Fundamental Aspects of Recalcitrance toward Antibiotics

Lebeaux

Ghigo

Beloin

2014

Microbiol Mol Biol Rev

1,050

862

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International audienceSurface-associated microbial communities, called biofilms, are present in all environments. Although biofilms play an important positive role in a variety of ecosystems, they also have many negative effects, including biofilm-related infections in medical settings. The ability of pathogenic biofilms to survive in the presence of high concentrations of antibiotics is called "recalcitrance" and is a characteristic property of the biofilm lifestyle, leading to treatment failure and infection recurrence. This review presents our current understanding of the molecular mechanisms of biofilm recalcitrance toward antibiotics and describes how recent progress has improved our capacity to design original and efficient strategies to prevent or eradicate biofilm-related infections

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Section: Biofilm Recalcitrance Is Multifactorialmentioning

confidence: 99%

Biofilm-Related Infections: Bridging the Gap between Clinical Management and Fundamental Aspects of Recalcitrance toward Antibiotics

Lebeaux

Ghigo

Beloin

2014

Microbiol Mol Biol Rev

1,050

862

View full text Add to dashboard Cite

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“…The proposed mechanism for resistance is that the glycocalyx may create a diffusion barrier to the antimicrobial agent de Beer et al, 1994;Chen and Stewart, 1996;Giwercman et al, 1991;Liu et al, 1998;Stewart et al, 1998). Diffusion through a biofilm may be affected by charge (ionic) interactions between the glycocalyx and the antimicrobial agent, by an increase in the distance the agent must diffuse, by molecular sieving (size exclusion), and by the viscosity of the glycocalyx.…”

Section: Limited Diffusion Through the Biofilmmentioning

confidence: 99%

Resistance mechanisms of bacteria to antimicrobial compounds

Cloete

2003

International Biodeterioration & Biodegradation

227

122

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“…Biofilm structure can prevent an applied antimicrobial agent from reaching the entire bacterial population by mechanisms such as a neutralizing reaction with components of the biofilm [29,10], synthesis of an antimicrobial degrading enzyme [16,3] and adsorption of the antimicrobial by the exo-polymeric substance (EPS) [19]. There have been several mathematical models of biofilm disinfection that include diffusion and various antimicrobialdegrading reactions [25,12,6].…”

Section: Introductionmentioning

confidence: 99%

Effects of persister formation on bacterial response to dosing

Cogan

2006

Journal of Theoretical Biology

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Almost all moist surfaces are colonized by microbial biofilms. Biofilms are implicated in cross-contamination of food products, biofouling and various human infections such as dental cavities, ulcerative colitis and chronic respiratory infections. The recalcitrance of biofilms to typical antibiotic and antimicrobial treatments is one focus of current investigations. Neither reaction-diffusion limitation nor heterogeneities in growth-rate explain the observed tolerance. Another hypothesis is that specialized 'persister' cells, which are extremely tolerant of antimicrobials, are the source of resistance.In this investigation, we describe the formation of 'persister' cells which neither grow nor die in the presence of antibiotics. We propose that these cells are of a different phenotype whose expression is regulated by the growth rate and the antibiotic concentration. Based on several experiments describing the dynamics of persister cells, we introduce a mathematical model that is used to describes the effect of a periodic dosing regiment. Results from our analysis indicate that the relative dose/withdrawal times are important in determining the effectiveness of such a treatment. A reduced model is also introduced and the similar behavior is demonstrated analytically.

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Induction of beta-lactamase production in Pseudomonas aeruginosa biofilm

Cited by 160 publications

References 14 publications

Biofilm-Related Infections: Bridging the Gap between Clinical Management and Fundamental Aspects of Recalcitrance toward Antibiotics

Biofilm-Related Infections: Bridging the Gap between Clinical Management and Fundamental Aspects of Recalcitrance toward Antibiotics

Resistance mechanisms of bacteria to antimicrobial compounds

Effects of persister formation on bacterial response to dosing

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