Induction of biliary cholangiocarcinoma cell apoptosis by 103Pd cholangial radioactive stent γ-rays

He, Gang; Sun, Dandan; DaWei, Ji; Sui, Dongming; Yu, Fa-Qiang; Gao, Qin-Yi; Dai, Xin; Gao, Hong; Jiang, Tao; Dai, Chaoliu

doi:10.1097/00029330-200806010-00011

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…Defects in the apoptotic machinery can lead to radiation resistance [16]. become an attractive avenue to improve the clinical response of cholangiocarcinoma patients [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Slug inhibition upregulates radiation-induced PUMA activity leading to apoptosis in cholangiocarcinomas

Zhang

et al. 2010

Med Oncol

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Resistance of cholangiocarcinoma to irradiation therapy is a major problem in cancer treatment. Slug, a snail family transcription factor, is a suppressor of PUMA (p53 upregulated modulator of apoptosis), which has been shown to be involved in the control of apoptosis. In this study, we investigated whether the modulation of Slug expression, using adeno-associated-virus-mediated transfer of siRNA targeting Slug gene (rAAV2-Slug siRNA), affects cholangiocarcinoma sensitivity to radiation. In the present study, we used rAAV2-Slug siRNA to downregulate the expression of Slug in QBC939 cholangiocarcinoma cell lines in vitro before γ-irradiation. In vivo studies were done with orthotopic cholangiocarcinoma, and radiosensitivity was evaluated both in vitro and in vivo. rAAV2-Slug siRNA transfection resulted in downregulation of the levels of Slug in QBC939 cells. In addition, rAAV2-Slug siRNA, in combination with radiation, increased levels of the PUMA, which contributes to the radiosensitivity of cholangiocarcinomas. Finally, treatment with rAAV2-Slug siRNA plus γ-irradiation completely regressed tumor growth in orthotopic cholangiocarcinomas model. In summary, integrating gene therapy with radiotherapy could have a synergistic effect, thereby improving the survival of patients with cholangiocarcinomas.

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“…Defects in the apoptotic machinery can lead to radiation resistance [16]. become an attractive avenue to improve the clinical response of cholangiocarcinoma patients [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Slug inhibition upregulates radiation-induced PUMA activity leading to apoptosis in cholangiocarcinomas

Zhang

et al. 2010

Med Oncol

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Dosimetric aspects of ¹⁰³Pd radioactive stent source

Sadeghi

Kiavar

2012

Kerntechnik

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This study aims to determine AAPM TG-60 dosimetric quantities in regions surrounding the 103Pd stent wall by MCNP5 Monte Carlo code. The Palmaz-Schatz stent was modeled by a hollow cylinder of 17.89mm length (2mm diameter) with net surface very similar to real stent. The Dose deposited per photon (Gy), relative dose, Anisotropy function, F(r,θ), and radial dose function, gL(r), were described at AAPM TG-60 protocol were generated from these values and listed in tabular format. For benchmarking, the relative dose values were verified with TG-43 and EGS4 code results at identical conditions, relative to the radial distances from surface of the stent. There were noticeable results. These physical dosimetric parameters can be used in future treatment planning systems for IVBT.

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Induction of biliary cholangiocarcinoma cell apoptosis by 103Pd cholangial radioactive stent γ-rays

Cited by 2 publications

References 13 publications

Slug inhibition upregulates radiation-induced PUMA activity leading to apoptosis in cholangiocarcinomas

Slug inhibition upregulates radiation-induced PUMA activity leading to apoptosis in cholangiocarcinomas

Dosimetric aspects of ¹⁰³Pd radioactive stent source

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Induction of biliary cholangiocarcinoma cell apoptosis by 103Pd cholangial radioactive stent γ-rays

Cited by 2 publications

References 13 publications

Slug inhibition upregulates radiation-induced PUMA activity leading to apoptosis in cholangiocarcinomas

Slug inhibition upregulates radiation-induced PUMA activity leading to apoptosis in cholangiocarcinomas

Dosimetric aspects of 103Pd radioactive stent source

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Dosimetric aspects of ¹⁰³Pd radioactive stent source