Induction of cytochrome p‐450 and phosphatidylcholine synthesis by endosulfan in liver of rats : Effect of quality of dietary proteins

Tyagi, S.; Sriram, Krishnan; Narayan, Satya; Misra, U. K.

doi:10.1080/03601238509372502

Cited by 3 publications

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References 18 publications

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“…In both mammals (Gupta & Gupta 1979, Tyagi et al 1985 and fish (Jensen et al 1991) endosulfan has been shown to function as a phenobarbital-type inducer of cytochrome P-450. In general, fish have repeatedly been shown not to respond to induction by phenobarbital-type compounds ( G o k s~y r et al 1987, Kleinow et al 1987, Gooch et al 1989, Winston et al 1989), although they express corresponding cytochrome P-450 IIB genes (Elskus & Stegeman 1989, Stegeman 1989, Miranda et al 1990; for nomenclature, see Nebert et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Cytological alterations in the liver of rainbow trout Oncorhynchus mykiss after prolonged exposure to low concentrations of waterborne endosulfan

Arnold¹,

Hj²,

Braunbeck³

1996

Dis. Aquat. Org.

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ABSTRACT-In order to elucidate sublethal cytopathological alterations in hepatocytes, mature male rainbow trout Oncorhynchus mykiss were exposed to 1, 10. 50, and 100 ng I-' technical grade endosulfan (ThiodanTM, 70% a-and 30 % P-isomers) for 28 d. Whereas stereological parameters, i.e. relative volumes and numbers of cell organelles, were unaffected, qualitative ultrastructural alterations were detectable from 10 ng I-' endosulfan. The No-Observed-Effect Concentration (NOEC) for cytological alterations was determined to be 1 ng I-' endosulfan, i e 3 orders of magnitude below the LC,, value Cytological effects that were probably of an adaptive nature included proliferation of SER and circular arrays of RER indicating induction of mixed-function oxygenases (MFO) as well as an increase in lysosomal elements at 50 and 100 ng I-' endosulfan, probably due to enhanced cellular turnover In addition, at 250 ng 1-' endosulfan, degenerative effects such as dilation of intermembranous spaces in mitochondria, deformation of mitochondria, myelin formation in peroxisomes and cytoplasm, and vesiculation and dilation of RER cisternae were observed. Although there was no indication of specific sublethal modes of toxic action except for MFO induction, the present study indicates that endosulfan has toxic impacts at concentrations of environmental relevance.

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Section: Introductionmentioning

confidence: 99%

Cytological alterations in the liver of rainbow trout Oncorhynchus mykiss after prolonged exposure to low concentrations of waterborne endosulfan

Arnold¹,

Hj²,

Braunbeck³

1996

Dis. Aquat. Org.

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Induction of phosphatidylcholine biosynthesis via cdpcholine pathway in lung and liver of rats following intratracheal administration of ddt and endosulfan

Narayan

Dani

Misra

1989

J. Biochem. Toxicol.

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The induction of phosphatidylcholine (PC) biosynthesis via the CDPcholine pathway in lung and liver of rats has been shown following the intratracheal administration of 1,1,1-trichloro-2m2-bis(p-chlorophenyl) ethane (DDT) (5 mg/100 g body weight) and endosulfan (1 mg/100 g body weight) for 3 days. Controls received only the vehicle solution (groundnut oil, 0.1 m1/100 g body weight). The treatment of DDT and endosulfan significantly increased the PC contents and the incorporation of radioactive [methyl-3H]choline into PC of lung and liver microsomes. The incorporation of radioactive [methyl-14C]methionine into microsomal PC of lung and liver was not affected significantly by treatment with either of the insecticides. 1,4,5,6,7-hexachloro-5-norbornene-2,3-dimethano cyclic sulfite (endosulfan) administration significantly increased the activity of choline kinase and phosphocholine cytidylyltransferase (both cytosolic and microsomal) of lung, whereas DDT increased the activity of only latter. In liver, both DDT and endosulfan administration significantly increased the activity of choline kinase and phosphocholine cytidylyltransferase (both cytosolic and microsomal). However, the activity of phosphocholinetransferase was not affected in both lung and liver microsomes of rats treated with these insecticides. The PC precursor pool sizes, choline and phosphorylcholine, of lung and liver tissues were not altered by DDT and endosulfan treatments. The present results suggest that the increased level of PC and incorporation of radioactive [methyl-3H]choline into microsomal PC could be the result of increased activity of choline kinase and phosphocholine cytidylyltransferase of lung and liver of rats following intratracheal administration of DDT and endosulfan.

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Response of xenobiotic metabolizing enzymes in rainbouw trout (Oncorhynchus mykiss) to endosulfan, detected by enzyme activities and immunochemical methods

et al. 1991

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Induction of cytochrome p‐450 and phosphatidylcholine synthesis by endosulfan in liver of rats : Effect of quality of dietary proteins

Cited by 3 publications

References 18 publications

Cytological alterations in the liver of rainbow trout Oncorhynchus mykiss after prolonged exposure to low concentrations of waterborne endosulfan

Cytological alterations in the liver of rainbow trout Oncorhynchus mykiss after prolonged exposure to low concentrations of waterborne endosulfan

Induction of phosphatidylcholine biosynthesis via cdpcholine pathway in lung and liver of rats following intratracheal administration of ddt and endosulfan

Response of xenobiotic metabolizing enzymes in rainbouw trout (Oncorhynchus mykiss) to endosulfan, detected by enzyme activities and immunochemical methods

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