Induction of Desiccation Tolerance in Plant Somatic Embryos: How Exclusive Is the Protective Role of Sugars?

Hoekstra, Folkert A.; Golovina, Elena A.; Tetteroo, F.A.A.; Wolkers, Willem F.

doi:10.1006/cryo.2001.2358

Cited by 72 publications

(63 citation statements)

References 45 publications

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“…The Natural Stress Solutes Have Little Impact on the Dehydrin Structure Under conditions of stress, plants are known to produce a variety of compatible solutes, such as betaine (Chen and Murata, 2002), Pro (Yoshiba et al, 1997), and different sugars (Ingram and Bartels, 1996;Hoekstra et al, 2001b). Of particular interest for this study are Suc and Glc, which are naturally produced in response to low temperature and drought (Hoekstra et al, 2001a).…”

Section: Analysis Of Structural Transitions Using Circular Dichroismmentioning

confidence: 99%

Mimicking the Plant Cell Interior under Water Stress by Macromolecular Crowding: Disordered Dehydrin Proteins Are Highly Resistant to Structural Collapse

2008

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The dehydrins are a class of drought-induced proteins in plants that lack a fixed three-dimensional structure. Their specific molecular action, as well as the reason for their disordered character, is as yet poorly understood. It has been speculated, however, that the dehydrins are tuned to acquire a biologically active structure only under the conditions in which they normally function (i.e. upon dehydration). To test this hypothesis, we here investigate the effect of reduced water content and macromolecular crowding on three dehydrins from Arabidopsis (Arabidopsis thaliana). As a simplistic model for mimicking cellular dehydration, we used polyethylene glycol, glycerol, and sugars that plants naturally employ as compatible solutes (i.e. sucrose and glucose). Macromolecular crowding was induced by the large polysaccharides Ficoll and dextran. The results show that the dehydrins are remarkably stable in their disordered state and are only modestly affected by the solvent alterations. A notable exception is the dehydrin Cor47, which shows a small, intrinsic increase in helical structure at high concentrations of osmolytes. We also examined the effect of phosphorylation but found no evidence that such posttranslational modifications of the dehydrin sequences modulate their structural response to osmolytes and crowding agents. These results suggest that the dehydrins are highly specialized proteins that have evolved to maintain their disordered character under conditions in which unfolded states of several globular proteins would tend to collapse.

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Section: Analysis Of Structural Transitions Using Circular Dichroismmentioning

confidence: 99%

Mimicking the Plant Cell Interior under Water Stress by Macromolecular Crowding: Disordered Dehydrin Proteins Are Highly Resistant to Structural Collapse

2008

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“…In plants, a group of very hydrophilic proteins, known as LATE EMBRYOGENESIS ABUNDANT (LEA) proteins, accumulate to high levels during the last stage of seed maturation (when acquisition of desiccation tolerance occurs in the embryo) and during water deficit in vegetative organs, suggesting a protective role during water limitation (Dure, 1993b;Bray, 1997;GarayArroyo et al, 2000;Hoekstra et al, 2001).…”

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confidence: 99%

The Enigmatic LEA Proteins and Other Hydrophilins

et al. 2008

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“…Nevertheless, plantlets seem to be capable of tolerating moderate concentrations of FMX (1 mM) by accumulating carbohydrates that are known to protect cell membranes against intense dehydration (Hoekstra et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Stress effects of flumioxazin herbicide on grapevine ( Vitis vinifera L.) grown in vitro

2003

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Among the herbicides used in vineyards, the pre-emergence soil-applied flumioxazin (FMX) is a recently synthesized molecule that inhibits chlorophyll biosynthesis in weed species. The aim of this work was to characterize the effects of FMX on non-target grapevine ( Vitis vinifera L. cv. Chardonnay) plantlets grown in vitro. FMX treatment (from 1 to 100 micro M) represented a stress, as revealed by measurement of several parameters. Stem and leaves underwent dehydration and a decrease in both water- and osmotic-potential. Treated plantlets exhibited concomitant accumulation of soluble carbohydrates in all tissues and of free proline in stems and leaves. Moreover, FMX caused lipid peroxidation and electrolyte leakage in leaf tissues. These results indicate that the herbicide FMX is toxic for grapevine grown in vitro. In addition to inhibiting protoporphyrinogen IX oxidase, it causes water stress and membrane alteration in tissues and, as a consequence, generates the accumulation of carbohydrates and free proline.

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Induction of Desiccation Tolerance in Plant Somatic Embryos: How Exclusive Is the Protective Role of Sugars?

Cited by 72 publications

References 45 publications

Mimicking the Plant Cell Interior under Water Stress by Macromolecular Crowding: Disordered Dehydrin Proteins Are Highly Resistant to Structural Collapse

Mimicking the Plant Cell Interior under Water Stress by Macromolecular Crowding: Disordered Dehydrin Proteins Are Highly Resistant to Structural Collapse

The Enigmatic LEA Proteins and Other Hydrophilins

Stress effects of flumioxazin herbicide on grapevine ( Vitis vinifera L.) grown in vitro

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