Induction of DNA double-strand breaks in primary gingival fibroblasts by exposure to dental resin composites

Urcan, Ebru; Scherthan, Harry; Styllou, Marianthi; Haertel, Uschi; Hickel, Reinhard; Reichl, Franz‐Xaver

doi:10.1016/j.biomaterials.2009.11.065

Cited by 117 publications

(117 citation statements)

References 28 publications

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“…The antioxidant concentration used was chosen on the basis that previous studies revealed a reduction of the TEGDMA and HEMA genotoxicity at a dose of 10 mM NAC 11,48,49) . As in our previous experiments we used cells exposed to 1 mM hydrogenperoxide (Sigma-Aldrich) dissolved in medium 28) and 0.5 Gy X-ray exposed cells 30) as a positive controls (not shown; see refs. 28,30).…”

Section: Chemicalsmentioning

confidence: 99%

“…As in our previous experiments we used cells exposed to 1 mM hydrogenperoxide (Sigma-Aldrich) dissolved in medium 28) and 0.5 Gy X-ray exposed cells 30) as a positive controls (not shown; see refs. 28,30).…”

Section: Chemicalsmentioning

confidence: 99%

“…In this study we used the half maximum effect concentration (EC 50) values for the investigated compounds as determined previously with the XTTbased cell viability assay for HGFs 28,30) .…”

Section: Xtt-based Viability Assaymentioning

confidence: 99%

“…In this respect the growing use of polymer composite material has been linked to adverse effects on gingiva and pulp homeostasis 11,26,27) . Previously, we observed that monomers can induce DNA DSBs in human gingiva fibroblasts (HGFs) using the DSB focus assay [28][29][30]. This assay makes use of the phosphorylation of histone H2AX (then called γ-H2AX) that is formed by DSB-responsive kinases in the chromatin surrounding a DSB [31][32][33][34] .…”

Section: Introductionmentioning

confidence: 99%

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NAC ameliorates dental composite-induced DNA double-strand breaks and chromatin condensation

Styllou

Hickel

et al. 2017

Dental Materials Journal

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Released (co)monomers from dental composite components can induce DNA damage of which DNA double-strand breaks (DSBs) threaten genome integrity. Here, we tested whether the administration of the antioxidant N-acetylcysteine (NAC) is able to reduce the dental composite-induced DSBs in primary human gingiva fibroblasts. The dental composites Bis-GMA (bisphenol-Aglycerolate dimethacrylate), GMA (glycidyl methacrylate), HEMA (2-hydroxyethyl methacrylate) and TEGDMA (triethyleneglycol dimethacrylate) were found to induce co-localizing microscopic nuclear foci numbers of the DSB markers γ-H2AX and 53BP1 per cell in the order: GMA>Bis-GMA>TEGDMA>HEMA. Supplementation of (co)monomer-containing culture medium with NAC led to a significant reduction of resin-induced DSBs as well as to an amelioration of dental monomer-induced nuclear chromatin condensation in gingival fibroblasts. Thus, antioxidant treatment can reduce radical-induced chromatin and DNA damage and open avenues to mitigate genotoxic effects of dental composite compounds.

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Section: Chemicalsmentioning

confidence: 99%

Section: Chemicalsmentioning

confidence: 99%

“…In this study we used the half maximum effect concentration (EC 50) values for the investigated compounds as determined previously with the XTTbased cell viability assay for HGFs 28,30) .…”

Section: Xtt-based Viability Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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NAC ameliorates dental composite-induced DNA double-strand breaks and chromatin condensation

Styllou

Hickel

et al. 2017

Dental Materials Journal

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“…Resinbased dental composites may release components from the resinous matrix, which could be initially due to partial polymerization, and/or later due to degradation processes over time [1][2][3][4] . These released components come into contact with oral tissues and may have various drawbacks like urticarial and mucosal reactions 5 , development of allergy and hypersensitivity reactions in patients 6 , modifications of gingival fibroblasts morphology and reduction on type I collagen protein 7 , immunosuppression or immunostimulation on mitogen-driven proliferation of purified T-lymphocytes and spleen cells 8 and DNA damage in primary human gingival fibroblasts, which underlines their genotoxic potential 9 . Many parameters can affect dental composite toxicity such as the shade of the composite, the light curing, the chemical composition of the resin monomer, the filler and the degree of conversion [10][11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Attik¹,

Gritsch²,

Colon³

et al. 2014

JoVE

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It is generally accepted that in vitro cell material interaction is a useful criterion in the evaluation of dental material biocompatibility. The objective of this study was to use 3D CLSM time lapse confocal imaging to assess the in vitro biocompatibility of dental composites. This method provides an accurate and sensitive indication of viable cell rate in contact with dental composite extracts. The ELS extra low shrinkage, a dental composite used for direct restoration, has been taken as example. In vitro assessment was performed on cultured primary human gingival fibroblast cells using Live/Dead staining. Images were obtained with the FV10i confocal biological inverted system and analyzed with the FV10-ASW 3.1 Software. Image analysis showed a very slight cytotoxicity in the presence of the tested composite after 5 hours of time lapse. A slight decrease of cell viability was shown in contact with the tested composite extracts compared to control cells. The findings highlighted the use of 3D CLSM time lapse imaging as a sensitive method to qualitatively and quantitatively evaluate the biocompatibility behavior of dental composites.

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Assessing the effect of triethyleneglycol dimethacrylate on tissue repair in 3D organotypic cultures

Tigani

Škrtić

Valerio

et al. 2018

J of Applied Toxicology

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Leachables from dental restoratives induce toxicity in gingival and pulp tissues and affect tissue regeneration/healing. Appropriate testing of these materials requires a platform that mimics the in vivo environment and allows the architectural self-assembly of cells into tissue constructs. In this study, we employ a new 3D model to assess the impact of triethyleneglycol dimethacrylate (TEGDMA) on early organization and advanced recruitment/accumulation of immortalized mouse gingival fibroblasts (GFs) and dental papilla mesenchymal cells (DPMCs) in extracellular matrix. We hypothesize that TEGDMA (1) interferes with the developmental architecture of GFs and DPMCs, and (2) inhibits the deposition of mineral. To test these hypotheses, GFs and DPMCs were incubated with the soluble TEGDMA at concentrations (0-2.5) mmol/L. Diameter and thickness of the constructs were determined by microscopic analysis. Cell differentiation was assessed by immunocytochemistry and the secreted mineral detected by alizarin-red staining. TEGDMA interfered with the development of GFs and/or DPMCs microtissues in a dose-dependent manner by inhibiting growth of inter-spherical cell layers and decreasing spheroid size (four to six times). At low/moderate TEGDMA levels, GFs organoids retained their structures while reducing thickness up to 21%. In contrast, at low TEGDMA doses, architecture of DPMC organoids was altered and thickness decreased almost twofold. Overall, developmental ability of TEGDMA-exposed GFs and DPMCs depended on TEGDMA level. GFs constructs were more resistant to structural modifications. The employed 3D platform was proven as an efficient tool for quantifying the effects of leachables on tissue repair capacities of gingiva and dental pulp.

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Induction of DNA double-strand breaks in primary gingival fibroblasts by exposure to dental resin composites

Cited by 117 publications

References 28 publications

NAC ameliorates dental composite-induced DNA double-strand breaks and chromatin condensation

NAC ameliorates dental composite-induced DNA double-strand breaks and chromatin condensation

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Assessing the effect of triethyleneglycol dimethacrylate on tissue repair in 3D organotypic cultures

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