NAC ameliorates dental composite-induced DNA double-strand breaks and chromatin condensation

Styllou, Panorea; Styllou, Marianthi; Hickel, Reinhard; Högg, Christof; Reichl, Franz‐Xaver; Scherthan, Harry

doi:10.4012/dmj.2016-316

Cited by 12 publications

(12 citation statements)

References 73 publications

(113 reference statements)

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“…Control, nontreated cells observed at concentrations determined to be cytotoxic in the XTT assay. These results are in accordance with previous reports on dental adhesive methacrylates [16,26]. The resulting DNA damage can be detected by the comet assay, micronucleus assay, chromosomal aberration, sisterchromatid exchange, and the γH2AX assay, all of which are used to analyze different genotoxic end points [42].…”

Section: Discussionsupporting

confidence: 91%

“…They have been reported to disrupt the cellular redox state of pulp cells in monolayer cultures and induce apoptosis and cell-cycle arrest of MDPC-23, undifferentiated pulp cells (OD-21), and macrophages [12][13][14][15]. With regard to the type of monomer, BisGMA has been shown to be highly toxic in close proximity to gingival fibroblasts, and TEGDMA induces apoptosis in cultured hGFs in a dose-and time-dependent manner [11,16,17]. HEMA was further shown to provoke accumulation of cells in the S-phase caused by DNA damage, and both HEMA and BisGMA induce oxidative DNA damage [18,19].…”

Section: Introductionmentioning

confidence: 99%

“…Overlapping single-strand breaks that arise from oxidative damage and overlapping BER patches can further cause DNA double-strand breaks (DSB) due to replication fork collapse [21]. In vitro experiments showed that antioxidants, such as ascorbic acid and Nacetylcysteine, reduce the level of DSBs following treatment with methacrylates, suggesting that DNA damage relies on the induction of ROS and the formation of epoxides rather than on the direct interaction of methacrylates (e.g., BisGMA, HEMA, TEGDMA, and UDMA) with the DNA [16,20,22,23]. Due to increased knowledge regarding DNA repair and damage responses, γH2AX-dependent approaches have evolved as a reliable tool for the detection of DNA damage in dental material sciences [22,24].…”

Section: Introductionmentioning

confidence: 99%

“…Nikolova et al pointed out that prior to testing materials with regard to their genotoxicity, their respective cytotoxicity should be assessed in order to establish the proper dose range [25]. Based on recent evidence [16,23,26], we decided to evaluate the genotoxic potential of seven commercially available light-cured, single-bottle orthodontic adhesives (Assure Plus, Assure Bonding Resin, ExciTE F, OptiBond Solo Plus, Scotchbond Universal Adhesive, Transbond MIP, and Transbond XT). Although polymerized dentin adhesives are known to exhibit a lower cytotoxicity than their unpolymerized counterparts, we consciously chose to test the adhesives in their uncured form to adhere to the in vivo conditions within the oral cavity.…”

Section: Introductionmentioning

confidence: 99%

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Genotoxic and cytotoxic potential of methacrylate-based orthodontic adhesives

Taubmann¹,

Willershausen

Al‐Maawi

et al. 2020

Clin Oral Invest

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Objectives The biocompatibility of methacrylate-based adhesives is a topic that is intensively discussed in dentistry. Since only limited evidence concerning the cyto- and genotoxicity of orthodontic adhesives is available, the aim of this study was to measure the genotoxic potential of seven orthodontic methacrylate-based adhesives. Materials and methods The XTT assay was utilized to determine the cytotoxicity of Assure Plus, Assure Bonding Resin, ExciTE F, OptiBond Solo Plus, Scotchbond Universal Adhesive, Transbond MIP, and Transbond XT after an incubation period of 24 h on human gingival fibroblasts. We also performed the γH2AX assay to explore the genotoxic potential of the adhesives within cytotoxic dose ranges after an incubation period of 6 h. Results The XTT assay showed a concentration-dependent reduction in cell viability. The decrease in cellular viability was in the same dose range most significant for Assure Plus, rendering it the adhesive material with the highest cytotoxicity. Employing the γH2AX assay, a concentration-dependent increase in H2AX phosphorylation was detected, indicating induction of DNA damage. Conclusions For most products, a linear correlation between the material concentration and γH2AX foci was observed. The most severe effect on γH2AX focus induction was found for Transbond MIP, which was the only adhesive in the test group containing the co-initiator diphenyliodonium hexafluorophosphate (DPIHP). Clinical relevance The data indicate that orthodontic adhesives, notably Transbond MIP, bear a genotoxic potential. Since the study was performed with in vitro cultivated cells, a direct translation of the findings to in vivo exposure conditions should be considered with great diligence.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genotoxic and cytotoxic potential of methacrylate-based orthodontic adhesives

Taubmann¹,

Willershausen

Al‐Maawi

et al. 2020

Clin Oral Invest

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“…nDNA possess unique types of injury with altered 3D chromatin architecture, rather than mtDNA [39]. Such a high-order chromatin structure is damaged by radical and prevented by antioxidant treatment [40].…”

Section: Types Of Damagementioning

confidence: 99%

The Role of DNA Repair in Maintaining Mitochondrial DNA Stability

Zhang

Reyes

Wang

2017

Advances in Experimental Medicine and Biology

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Mitochondria are vital double-membrane organelles that act as a "powerhouse" inside the cell and have essential roles to maintain cellular functions, e.g., ATP production, iron-sulfur synthesis metabolism, and steroid synthesis. An important difference with other organelles is that they contain their own mitochondrial DNA (mtDNA). Such powerful organelles are also sensitive to both endogenous and exogenous factors that can cause lesions to their structural components and their mtDNA, resulting in gene mutations and eventually leading to diseases. In this review, we will mainly focus on mammalian mitochondrial DNA repair pathways that safeguard mitochondrial DNA integrity and several important factors involved in the repair process, especially on an essential pathway, base excision repair. We eagerly anticipate to explore more methods to treat related diseases by constantly groping for these complexes and precise repair mechanisms.

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Impact of bisphenol A and analogues eluted from resin‐based dental materials on cellular and molecular processes: An insight on underlying toxicity mechanisms

Rajkumar,

Padmanaban

2024

J of Applied Toxicology

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Dental resin systems, used for artificial replacement of teeth and their surrounding structures, have gained popularity due to the Food and Drug Administration's (FDA) recommendation to reduce dental amalgam use in high‐risk populations and medical circumstances. Bisphenol A (BPA), an endocrine‐disrupting chemical, is an essential monomer within dental resin in the form of various analogues and derivatives. Leaching of monomers from resins results in toxicity, affecting hormone metabolism and causing long‐term health risks. Understanding cellular‐level toxicity profiles of bisphenol derivatives is crucial for conducting toxicity studies in in vivo models. This review provides insights into the unique expression patterns of BPA and its analogues among different cell types and their underlying toxicity mechanisms. Lack of a consistent cell line for toxic effects necessitates exploring various cell lines. Among the individual monomers, BisGMA was found to be the most toxic; however, BisDMA and BADGE generates BPA endogenously and found to elicit severe adverse reactions. In correlating in vitro data with in vivo findings, further research is necessary to classify the elutes as human carcinogens or xenoestrogens. Though the basic mechanisms underlying toxicity were believed to be the production of intracellular reactive oxygen species and a corresponding decline in glutathione levels, several underlying mechanisms were identified to stimulate cellular responses at low concentrations. The review calls for further research to assess the synergistic interactions of co‐monomers and other components in dental resins. The review emphasizes the clinical relevance of these findings, highlighting the necessity for safer dental materials and underscoring the potential health risks associated with current dental resin systems.

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NAC ameliorates dental composite-induced DNA double-strand breaks and chromatin condensation

Cited by 12 publications

References 73 publications

Genotoxic and cytotoxic potential of methacrylate-based orthodontic adhesives

Genotoxic and cytotoxic potential of methacrylate-based orthodontic adhesives

The Role of DNA Repair in Maintaining Mitochondrial DNA Stability

Impact of bisphenol A and analogues eluted from resin‐based dental materials on cellular and molecular processes: An insight on underlying toxicity mechanisms

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