Induction of DNA synthesis in rat macrophages in vitro by inflammatory exudate

Adolphe, M; J, Fontagné; Pelletier, Monique; Jp, Giroud

doi:10.1038/253637a0

Cited by 49 publications

(14 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…y.P. Giroud et al pieural exudates induced by dextran (Adolphe et al, 1975) or calcium pyrophosphate and k carrageenan, stimulated normal macrophages to divide in vitro (Giroud et al, 1977).…”

mentioning

confidence: 99%

Acute non-specific inflammation and modification of macrophage and lymphocyte funtions

Giroud

Sheng

Pelletier

et al. 2006

British Journal of Dermatology

Self Cite

View full text Add to dashboard Cite

SUMMARY The acute inflammatory response is a common phenomenon experienced by the physician in a wide variety of clinical situations. One of the major problems facing the investigator in this field of research who wishes to look at the cellular and humoral responses involved, is that in most of the animal models previously used, the inflammatory reaction has been provoked in subcutaneous tissues. Thus, a quantitative assessment of leucocyte emigration and mediator production in the inflamed area is technically difficult without resorting to complicated and artificial methods. Inflammation provoked in the pleural cavity provides a useful tool in the study of these problems since the collection of cells and analysis of humoral factors in exudates is easily accomplished. Among the irritants that may be used, we focused our interest on calcium pyrophosphate (CaPP) crystals, a non‐diffusible, non‐antigenic and endotoxin‐free irritant, because their deposition is implicated in pseudogout and chondrocalcinosis in man (MacCarty, 1973). Calcium pyrophosphate‐induced pleurisy is typified by an acute reaction, dominated by polymorphonuclear leucocytes, reaching its maximal intensity at about 5 h and disappearing within 48 h (Willoughby et al., 1975). It was found to be independent of the complement system. Examination of the known mediators of inflammation revealed no significant participation of either histamine or 5‐hydroxytryptamine. There was an early rise in PGE2 followed by a greater rise in PGF2a as the reaction diminished (Capasso et al., 1975). More recently, we have demonstrated the presence of a small quantity of thromboxane and a large quantity of prostacyclin during the first 2 hours of this inflammatory process. It was also shown that this type of acute inflammation was accompanied by the very rapid liberation of acute phase proteins both locally, in the exudate, and systemically in the serum (Tissot et al., 1983). Some of these events were also examined during the pleural reaction to other types of irritants (Capasso et al., 1975) and gave similar results (Tissot et al., 1983).

show abstract

mentioning

confidence: 99%

Acute non-specific inflammation and modification of macrophage and lymphocyte funtions

Giroud

Sheng

Pelletier

et al. 2006

British Journal of Dermatology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The physiologic significance of the latter function is not clear at present but it may serve to provide more active macrophages locally. In fact, the replication of macrophages at the site of inflammation has been well documented (17) and inflammatory exudates have been shown to contain the growth factor required for the replication of mononuclear phagocytes (18,19).…”

Section: Discussionmentioning

confidence: 99%

Conversion of monocytes to cells capable of anchorage-independent growth in vitro.

Lin¹,

Gordon²,

Chen³

et al. 1981

The Journal of Experimental Medicine

View full text Add to dashboard Cite

We investigated the time-course involved in the conversion of mouse blood monocytes in vitro in cells capable of anchorage-independent growth. Two criteria were used to define when monocytes were fully converted to cells similar to mononuclear phagocytes present in inflammatory exudate, such as thioglycollate medium (TM)-elicited peritoneal exudate. They were the production of high levels of plasminogen activators and an ability to undergo anchorage-independent growth. Resident peritoneal macrophages were used as controls and for comparison. Our studies indicated that monocytes, but not resident peritoneal macrophages, could be converted to cells similar to TM-elicited mononuclear phagocytes after 2 d in culture.

show abstract

“…(10) found DNA synthesis in adherent macrophages cultured in a medium containing such supernatants. In addition, two other groups (1,32) recently reported that they had induced macrophage proliferation in vitro by addition of inflammation exudate. A more exact nature of the mitogenic factor was not explored in those studies.…”

Section: Discussionmentioning

confidence: 99%

The Macrophage Response in Mice After Primary and Secondary Immunization With Sheep Red Blood Cells

Reikvam

1976

Acta Pathologica Microbiologica Scandinavica Section C Immunolo

View full text Add to dashboard Cite

Reikvam, A. The macrophage response in mice after primary and secondary immunization with sheep red blood cells. Acta path. microbiol. scand. Sect. C, 84: [161][162][163][164][165][166][167] 1976.A primary and a secondary immune response to sheep red blood cells (SRBC) was evoked in mice by intraperitoneal injection of the antigen. Macrophage proliferation and migration as well as induction of the lysoromal enzyme acid phosphatase in macrophages were assessed along with lymphocyte proliferation. The proliferative responses were evaluated by 3H-thymidine labelling (autoradiography) . The secondary response elicited a faster and stronger lymphocyte proliferation than the primary response. This pattern was not seen to apply to the macrophages where the highest proliferative rate was found in connection with the primary response. 'The macrophages acquired an increased content of acid phosphatase during both types of responses, but the significantly highest level was reached in the secondary response. Large numbers of monocytes migrated into the peritoneal cavity during the first day after immunization. However, macrophage proliferation took place in resident as well as in immigrant cells.

show abstract

Induction of DNA synthesis in rat macrophages in vitro by inflammatory exudate

Cited by 49 publications

References 6 publications

Acute non-specific inflammation and modification of macrophage and lymphocyte funtions

Acute non-specific inflammation and modification of macrophage and lymphocyte funtions

Conversion of monocytes to cells capable of anchorage-independent growth in vitro.

The Macrophage Response in Mice After Primary and Secondary Immunization With Sheep Red Blood Cells

Contact Info

Product

Resources

About