Induction of embryogenic callus and cell suspension culture from shoot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae)

Tokuhara, Ken; Mii, Masahiro

doi:10.1007/s11627-001-0080-4

Cited by 76 publications

(50 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is a natural growth promoter which contains higher levels of zeatin, zeatin ribosides, 1,3-diphenylurea (contains cytokinin like activity), auxins, nitrogenous compounds, inorganic elements, organic acids, sugars and their alcohols, peptides, vitamins, amino acids and many other unknown components in its composition (Tokuhara and Mii, 2001;Nasib et al, 2008). George et al, (2008) demonstrated that physiologically active substances presents in CW promote the cell divisions which further enhance shoot multiplication.…”

Section: Medium Supplementsmentioning

confidence: 99%

A Review on: In Vitro Cloning of Orchids

Sarmah¹,

Kolukunde²,

Sutradhar³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Section: Medium Supplementsmentioning

confidence: 99%

A Review on: In Vitro Cloning of Orchids

Sarmah¹,

Kolukunde²,

Sutradhar³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…In most of plant species, regeneration of plants from callus tissue, especially highly totipotent embryogenic callus, has been recognized as one of the essential techniques for micropropagation and biotechnological applications (Tokuhara and Mii, 2001). Therefore, the interest in using these techniques for rapid and largescale propagation of medicinal and aromatic plants has been significantly increased (Sahoo et al, 1997;.…”

Section: Introductionmentioning

confidence: 99%

High Frequency of Plant Regeneration through Adventitious Shoots Proliferation from Leaf Explant of Patchouli–Pogostemon cablin (Blanco) Benth

Gopi¹

2017

Int. J. Curr. Res. Biosci. Plant Biol.

View full text Add to dashboard Cite

A b s t r a c t A r t i c l e I n f oPlant regeneration potential through adventitious shoots proliferation was studied from leaf explant of evergreen perennial aromatic herb, patchouli plant, or Pogostemon cablin. Leaf explant showed differentiation of embryogenic callus on Murashige and Skoog's medium (MS) supplemented with 2,4-D 0.5 mg L -1 , 3% sucrose and 0.9% agar. Embryogenic callus was further transfer to same medium with BA 0.5 mg L -1 produced somatic embryos and adventitious shoots having dark green colour after four weeks. The maximum range of embryogenesis frequency was 89%, depending on the BAP concentration in combination with 2,4-D. The best response was observed on MS medium containing 0.5 mg L -1 BAP along with 0.5 mg L -1 2,4-D. The embryos or adventitious shoots matured and developed on fresh MS medium with growth regulators. Maximum numbers of 74 adventitious shoots germination were observed and developed further for root induction on the medium containing 0.5 mg/l BAP and 0.5 mg/l NAA after six weeks of culture. Regenerated shoots were separated and rooted on same half strength MS medium supplemented with 1.0 mg/l of NAA alone for three weeks. Approximately 30-105 plantlets were regenerated from each leaf explant. Well-regenerated plants grew vigorously after transplanting to a soil-less container in shade house.

show abstract

“…Different types of plant growth regulators (PGRs) and sources of explants have been used to induce PLBs. PLB induction on MS medium with addition of BAP and NAA [7] or TDZ [4] using leaf explants from plantlets have been reported. The use of 5.4 µM NAA and 4.5 µM TDZ has been applied to induce PLBs and plantlets from flower stalks of Doritaenopsis orchids [8].…”

Section: Introductionmentioning

confidence: 99%

Regeneration of Plantlets Through PLB (Protocorm-Like Body) Formation in Phalaenopsis ‘Join Angle X Sogo Musadian’

Meilasari¹,

Iriawati²

2016

j.math.fund.sci.

View full text Add to dashboard Cite

Abstract. Selection and regeneration of specific hybrids of Phalaenopsis typically takes a long time since this plant usually reproduces through seeds. This study was conducted to examine the best medium and explants for regeneration of Phalaenopsis 'Join Angle x Sogo Musadian'. In vitro and in vivo roots and leaves were used as explants, which were cultured in half-strength Murashige and Skoog medium supplemented with various combinations of plant growth regulators (TDZ, 2,4-D, NAA, BAP and IAA). The results showed that the in vitro roots produced the highest number of PLBs (49.3 PLBs) when they were cultured in medium containing 0.5 ppm NAA, 5 ppm BAP, and 0.5 ppm IAA. The in vitro leaf explants also regenerated PLBs, however, only two PLBs developed, i.e. when they were cultured in medium containing 1 ppm TDZ and 2 ppm 2,4-D. PLB germination into plantlets was performed by culturing each PLB on MS medium without plant growth regulators. Based on the results of the present study, it can be concluded that in vitro roots and half-strength MS medium supplemented with 0.5 ppm NAA, 5 ppm BAP, and 0.5 ppm IAA are the best explant and best medium respectively for plant regeneration through PLBs.

show abstract

Induction of embryogenic callus and cell suspension culture from shoot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae)

Cited by 76 publications

References 5 publications

A Review on: In Vitro Cloning of Orchids

A Review on: In Vitro Cloning of Orchids

High Frequency of Plant Regeneration through Adventitious Shoots Proliferation from Leaf Explant of Patchouli–Pogostemon cablin (Blanco) Benth

Regeneration of Plantlets Through PLB (Protocorm-Like Body) Formation in Phalaenopsis ‘Join Angle X Sogo Musadian’

Contact Info

Product

Resources

About