Induction of embryogenic Triticum aestivum L. calli. I. Quantification of genotype and culture medium effects

Carman, John G.; Jefferson, Nancy E.; Campbell, W. F.

doi:10.1007/bf00035908

Cited by 42 publications

(28 citation statements)

References 20 publications

(32 reference statements)

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“…Dicamba was effective in inducing callus from embryo culturing of wheat (Triticum aestivum L.) 38 . A similar result was also found in immature embryo cultures of winter wheat 39 and spring wheat cultivars 40 . Dicamba is a promising auxin which has been reported to be effective in promoting direct and indirect embryogenic callus induction from cultured mature zygotic embryo and young of leaf oil palm 41 .…”

Section: Discussionsupporting

confidence: 85%

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Thawaro¹,

Te-chato²

2009

ScienceAsia

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ABSTRACT:Randomly amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) were used to assess the hybrid verification and somaclonal variation of the cross 366 (D) × 72 (P) of Elaeis quineensis Jacq. DxP. DNA from mature zygotic embryo (MZE) was isolated and detected by seven primers of RAPD and eight primers of SSR. Half MZEs consisting of coleoptiles regions were cultured on Murashige and Skoog (MS) medium plus various kinds and concentrations of auxins. Variation of somaclone obtained through somatic embryogenesis was detected at the proliferation stage of the culture period. The result revealed that all random primers and the SSR primers tested could amplify parental DNA. Primer OPT06 (for RAPD) and EgCIR1772 (for SSR) provided clear DNA patterns and could be used for verifying the hybridity of the cross 366 (D) × 72 (P). The highest frequency of nodular callus formation at 87.5% was obtained on MS medium supplemented with 2.50 mg/l of 3,6-dichloro-o-anisic acid (dicamba). This was significantly different from other kinds and concentrations of auxins. Somatic embryo line at globular stage derived from each half MZE showed no variation in RAPD banding patterns with primer OPT06. It was concluded that no somaclonal variation occurred in our propagation system by RAPD marker, no major genetic changes were observed, and this augurs well for the propagation system being employed since somaclonal variation may be minimized or absent altogether.

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Section: Discussionsupporting

confidence: 85%

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Thawaro¹,

Te-chato²

2009

ScienceAsia

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“…Mean squares from combining ability analysis for in vitro traits of primary and secondary cultures been reported to be dependent upon the genotype and medium protocols (Barakat & Cocking, 1989) . Several investigators (Sears & Dechard, 1982 ;Lazar et al ., 1983 ;Maddock et al ., 1983 ;Mathias & Simpson, 1986 ;Carman et al ., 1988 ;Bhaskaran & Smith, 1990) have recognized that among wheat genotypes there is a significant variation in the callus initiation and shoot formation .…”

Section: Resultsmentioning

confidence: 99%

Combining abilities of in vitro traits in wheat (Triticum aestivum L.) immature embryo cultures

Barakat

1994

Euphytica

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Combining ability for six in vitro culture traits in wheat were studied in a 8 x 8 diallel cross (excluding reciprocals) . Specific combining ability effects (sca) were significant for all six traits derived from immature embryos on two media protocols, whereas general combining ability (gca) variances were significant only for five of them. Furthermore, based on ratios obtained by comparing the ratio of K2 gca to K 2 sca, sca was more important than gca for all six traits . Genetic correlations between shoot formation and other in vitro traits, except callus weight and root formation, were higher in magnitude than the corresponding phenotypic correlations estimates, indicating the importance of genetic effects .

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“…The precise response of wheat explant tissues to a regime of tissue culture is highly genotype dependent and extensive variation has been reported Carman et al 1988;Fennell et al 1996;Machii et al 1998;Maddock et al 1983;Redway et al 1990;Sears and Deckard 1982;Shimada 1978;Viertel et al 1998). Some wheat cultivars are more responsive and regenerable than others.…”

Section: Genotype Dependencymentioning

confidence: 95%

Wheat Biotechnology: Current Status and Future Prospects

Jones

2015

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

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In 2010, wheat was grown on nearly 217 million ha worldwide (more than any other food crop) and a total of 651 million t of grain was harvested, making it the third most-cultivated cereal after maize and rice (www.faostat.fao.org). It is one of the main staple food crops providing approximately 20 % of our daily needs of protein and calories. Wheat is traded on global commodity markets which have become accustomed to a good annual harvest and large reserves. However, global demand, estimated at 666 million metric tons (MMT) in 2010, is predicted to increase in line with population growth and reach 880 MMT (a 40 % increase) by 2050 (Weigand 2011). Significant action will be required to maintain this positive situation.Further complicating the supply/demand balance are extreme weather events, unpredictable longer-term climate patterns and changing regional preferences in crop choice/suitability. Recent localised severe weather and poor harvests have resulted in price volatility with notable spikes in 2008 and 2012 (Fig. 8.1). The volatility in the wheat market was exacerbated by the Russian export ban in 2010 resulting from an extreme heatwave in the grain producing regions of southwestern Russia. The highest July temperatures in 130 years brought severe drought, causing the annual harvest of the biggest wheat producing region (Volga) to be reduced by more than 70 % on that of the previous year (Wegren 2011; Welton 2011). The US wheat supplies for 2013/2014 are projected at 2917 million bushels, down 7 % from 2012/2013 (Vocke and Liefert 2014) and there is evidence that growers in some US states are moving away from wheat in favour of biotech soya and corn. Quoting statistics from the US Department of Agriculture, Rookhuyzen (2012) stated that wheat acreage for South Dakota declined by 1.2 million acres, or 28 %, between 1981 and 2011 and the acres planted with spring wheat in 2012 were the lowest in

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Induction of embryogenic Triticum aestivum L. calli. I. Quantification of genotype and culture medium effects

Cited by 42 publications

References 20 publications

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Combining abilities of in vitro traits in wheat (Triticum aestivum L.) immature embryo cultures

Wheat Biotechnology: Current Status and Future Prospects

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