Induction of endophytic colonization in rice (Oryza sativa L.) tissue culture plants by Azorhizobium caulinodans

Abstract

Endophytic colonization in rice was induced using rhizobia. Dehusked seeds of rice hybrid, CORH2, were used as explants for induction of calli. MS medium was modified with 2,4-D (2.5 mg l(-1)) and kinetin (0.2 mg l(-1)) for callus induction. Well-developed calli were inoculated with Azorhizobium caulinodans strains ORS 571 and AA-SK-5 by means of imbibition. All treated calli had significant increases in protein content, total nitrogen and nitrogenase activity. Imbibition of ORS 571 had significant biochemical effect on the developing calli than AA-SK-5. The crop response study from the regenerated plantlets showed a positive correlation in yield than uninoculated control. The endophytic colonization was observed in all parts of the plants analyzed. Further, colonization was also confirmed by microtome sectioning.

Acclimatization of tissue cultured plantlets: from laboratory to land

“…Research community should focus on understanding of plant microbe interactions to optimize the beneficial plant-endophyte bacterial relationships. Senthilkumar et al (2008) …”

“…Endophytic colonization in rice (Oryza sativa L.) tissue culture plants (Fig. 1) by using rhizobia has been studied by Senthilkumar et al (2008). Calli treated with Azorhizobium caulinodans strains ORS571 and AA-SK-5 showed colonization in almost all parts of plant with significant increases in protein content, total nitrogen and nitrogenase activity.…”

In vitro multiplication of Dianthus carthusianorum calamine ecotype with the aim to revegetate and stabilize polluted wastes

“…One of them is the low root system ability to compete with antagonistic microbial soil communities. It is therefore necessary to accustom the plants to such unfavourable conditions by biotization of tissue cultured plantlets with useful microorganisms that promote growth and encourage mutual association (Adriaensen et al 2003;Senthilkumar et al 2008;Parray et al 2015;Quambusch et al 2016). During our experiments, well-rooted microplants representing calamine ecotype of D. carthusianorum, after efficacious acclimatization to greenhouse conditions, were successfully transplanted to contaminated substratum without any additional treatments.…”

Identity, Diversity, and Molecular Phylogeny of the Endophytic Mycobiota in the Roots of Rare Wild Rice ( Oryza granulate ) from a Nature Reserve in Yunnan, China

“…Its consumption exceeds 100 kg per capita annually in many Asian countries, and it is the principal food for most of the world's poorest people, particularly in Asia. The association of arbuscular mycorrhizal fungi and endophytic bacteria with rice plants has been well documented (15,32,35,44,53,56,60). Less, however, is known about its fungal endophytes.…”