Induction of G2/M phase arrest and apoptosis by a novel enediyne derivative, THDB, in chronic myeloid leukemia (HL-60) cells

Lu, Yu‐Jhang; Yang, Sheng-Huei; Chien, Ching-Ming; Lin, Yi‐Hsiung; Hu, Xiu-Wei; Wu, Zchong-Zcho; Wu, Ming‐Jung; Lin, Shinne‐Ren

doi:10.1016/j.tiv.2006.09.008

Cited by 37 publications

(28 citation statements)

References 27 publications

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“…6A). The levels of CDK2 and cyclin A, essential proteins in the mitotic process (27), decreased with time post-exposure of MCF-7 cells to p28 (Fig. 6A).…”

Section: Modulation Of Cell Cycle-related Proteins By P28mentioning

confidence: 96%

A peptide fragment of azurin induces a p53-mediated cell cycle arrest in human breast cancer cells

Yamada

Mehta

Lekmine

et al. 2009

Molecular Cancer Therapeutics

149

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We report that amino acids 50 to 77 of azurin (p28) preferentially enter the human breast cancer cell lines MCF-7, ZR-75-1, and T47D through a caveolin-mediated pathway. Although p28 enters p53 wild-type MCF-7 and the isogenic p53 dominant-negative MDD2 breast cancer cell lines, p28 only induces a G 2 -M-phase cell cycle arrest and apoptosis in MCF-7 cells. p28 exerts its antiproliferative activity by reducing proteasomal degradation of p53 through formation of a p28:p53 complex within a hydrophobic DNA-binding domain (amino acids 80-276), increasing p53 levels and DNA-binding activity. Subsequent elevation of the cyclin-dependent kinase inhibitors p21 and p27 reduces cyclin-dependent kinase 2 and cyclin A levels in a time-dependent manner in MCF-7 cells but not in MDD2 cells. These results suggest that p28 and similar peptides that significantly reduce proteasomal degradation of p53 by a MDM2-independent pathway(s) may provide a unique series of cytostatic and cytotoxic (apoptotic) chemotherapeutic agents.

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“…6A). The levels of CDK2 and cyclin A, essential proteins in the mitotic process (27), decreased with time post-exposure of MCF-7 cells to p28 (Fig. 6A).…”

Section: Modulation Of Cell Cycle-related Proteins By P28mentioning

confidence: 96%

A peptide fragment of azurin induces a p53-mediated cell cycle arrest in human breast cancer cells

Yamada

Mehta

Lekmine

et al. 2009

Molecular Cancer Therapeutics

149

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“…Many anticancer agents have been reported to arrest the cell cycle at a specific checkpoint, and thereby induce apoptotic cell death (25,26). We found that alantolactone acts as an inhibitor of K562/ADR cell growth.…”

Section: Discussionmentioning

confidence: 69%

Alantolactone inhibits growth of K562/adriamycin cells by downregulating Bcr/Abl and P‐glycoprotein expression

Yang

Sun

et al. 2013

IUBMB Life

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Alantolactone, a sesquiterpene lactone containing an a-methylene-c-lactone group, is the active component of Inula helenium (Compositae), a traditional Chinese medicinal herb. It has been reported that alantolactone has the capacity to inhibit tumor cell growth through induction of apoptosis. The purpose of this study was to assess the effects of alantolactone in the adriamycin (ADR)-resistant human erythroleukemia cell line K562/ADR, and provide evidence that it might function as a potent therapeutic agent in chronic myelogenous leukemia (CML) patients with Bcr/Abl and the multidrug-resistance phenotype. Our results showed that alantolactone significantly inhibited K562/ADR cell growth by downregulating Bcr/Abl and P-glycoprotein expression. Alantolactone also induced apoptosis via modulation of protein levels of Bcl-2 family members, caspase activation, poly ADP ribose polymerase cleavage, and cytochrome C release. We also observed that alantolactone induced cell-cycle arrest in the G2/M phase, downregulated cyclin B1 and cyclin-dependent protein kinase 1, and upregulated the cyclin-dependent kinase inhibitor p21.Together, these results demonstrate that alantolactone may be a potent therapeutic agent against CML, and a potential Bcr/Abl inhibitor. V C 2013 IUBMB Life, 65(5): 435-444, 2013.

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“…The normal growth of the cell depends on strictly controlled stages of the cell cycle (10). According to the present study, HT has the potential to influence the cell cycle stage, and a number of anti-cancer agents have previously been reported to arrest the cell cycle at specific points, and thereby induce apoptotic cell death (11)(12)(13). The cell cycle is arrested at the G2 phase when DNA is damaged (14,15).…”

Section: Discussionmentioning

confidence: 70%

Juglans mandshurica Maxim extracts exhibit antitumor activity on HeLa cells in vitro

Xin

Hasan

et al. 2014

Molecular Medicine Reports

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Abstract. The present study examined the potential application of Juglans mandshurica Maxim extracts (HT) for cancer therapy by assessing their anti-proliferative activity, reduction of telomerase activity, induction of apoptosis and cell cycle arrest in S phase in HeLa cells. From the perspective of using HT as a herbal medicine, photomicroscopy and florescent microscopy techniques were utilized to characterize the effect of the extracts on telomerase activity and cell morphology. Flow cytometry was employed to study apoptosis and cell cycle of HeLa cells, and DNA laddering was performed. The results showed that HT inhibited cell proliferation and telomerase activity, induced apoptosis and caused S phase arrest of HeLa cells in vitro. HT inhibited HeLa cell proliferation significantly, and the highest inhibition rate was 83.7%. A trap-silver staining assay showed that HT was capable of markedly decreasing telomerase activity of HeLa cells and this inhibition was enhanced in a time-and dose-dependent manner. Results of a Hoechst 33258 staining assay showed that HeLa cells treated by HT induced cell death. Through DNA agarose gel electrophoresis, DNA ladders of HeLa cells treated with HT were observed, indicating apoptosis. In conclusion, the present study demonstrated that HT exhibited anti-tumor effects comprising the inhibition of growth and telomerase activity as well as apoptosis and cell cycle arrest in HeLa cells.

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Induction of G2/M phase arrest and apoptosis by a novel enediyne derivative, THDB, in chronic myeloid leukemia (HL-60) cells

Cited by 37 publications

References 27 publications

A peptide fragment of azurin induces a p53-mediated cell cycle arrest in human breast cancer cells

A peptide fragment of azurin induces a p53-mediated cell cycle arrest in human breast cancer cells

Alantolactone inhibits growth of K562/adriamycin cells by downregulating Bcr/Abl and P‐glycoprotein expression

Juglans mandshurica Maxim extracts exhibit antitumor activity on HeLa cells in vitro

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