Induction of geranylgeranyl diphosphate synthase activity and taxane accumulation in Taxus baccata cell cultures after elicitation by methyl jasmonate

Laskaris, Gregory; Bounkhay, Mina; Theodoridis, Georgios; Heijden, Robert van der; Verpoorte, Robert; Jaziri, Mondher El

doi:10.1016/s0168-9452(99)00078-3

Cited by 44 publications

(23 citation statements)

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“…It has been reported that MeJA induces production of taxol and other taxoids with a concomitant increase in GGDP synthase and other enzymes involved in taxol biosynthesis. 19) In addition, Martin et al showed that the increased accumulation of resin terpenoids (monoterpenoids and diterpenoids) in Norway spruce was parallel to the increase in prenyltransferase or/and terpenoid synthases.27) Therefore, MeJA might selectively stimulate biosynthetic enzymes such as GGDP synthase and/or GGDP cyclase and other enzymes involved in SDB production in S. dulcis. …”

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“…[17][18][19] Thus, we examined the dose-dependent effect of MeJA on the growth of the cultured tissues. The indicated concentration of MeJA was added to the medium at day 6 and cultured for 4 d. Although the growth of the tissues was not noticeably inhibited in response to 50 mM or less MeJA, a significant inhibition in growth (pϽ0.05) was observed when the tissues were treated with 100 mM MeJA ( Table 1).…”

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Selective Enhancement of Scopadulcic Acid B Production in the Cultured Tissues of Scoparia dulcis by Methyl Jasmonate

Nkembo

Lee

Hayashi

2005

CHEMICAL & PHARMACEUTICAL BULLETIN

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The effects of methyl jasmonate (MeJA) on isoprenoid production were evaluated in cultured tissues of Scoparia dulcis. It was found that MeJA suppressed the accumulation of chlorophylls, carotenoids, phytol and b bsitosterol in the tissues. MeJA, however, remarkably enhanced the production of scopadulcic acid B (SDB) , with 10 m mM being optimal observed concentration for stimulation of SDB production. The maximum concentration of SDB was observed 6 d after MeJA treatment.Key words scopadulcic acid B; Scoparia dulcis; methyl jasmonate; diterpene; biosynthesis Chart 1 100 mM MeJA, respectively. A similar result was observed for the content of carotenoids. A significant reduction of chlorophyll content and carotenoids was observed even at 10 mM MeJA. This rapid breakdown of chlorophylls and carotenoids in the tissues treated with MeJA was consistent with the results of experiments using Arabidopsis thaliana, 21) and it could be explained by the chlorophyllase gene AtCLH1 inductive effect, which is known to promote senescence and chlorophyll degradation in plants. 22) An SDB-type plants of S. dulcis produce SDB as a major diterpene together with b-sitosterol.13) Since exogenously supplied MeJA has been reported to elicit accumulation of secondary metabolites, 15,23) the effects of MeJA on the production of SDB, phytol and b-sitosterol were evaluated. A dose-dependent decrease in the total content of phytol and bsitosterol was observed in the tissues treated with MeJA, while SDB content in the tissues increased solely in the presence of 10 mM MeJA. No significant change was induced at other concentrations. Only this condition significantly enhanced the SDB production (pϽ0.01) when compared with the control or other conditions. It was shown that wounding of plant tissues induced hmg1 gene that encodes HMGR enzymes involved in the biosynthesis of phytosterols and its induction was suppressed by elicitors in a potato 24) and methyl jasmonate in Camptotheca acuminata.25) These findings might explain the decrease in the content of b-sitosterol in the tissues treated with MeJA. In our previous communication, the involvement of 1-deoxy-D-xylulose 5-phosphate (DOXP) pathway, so called mevalonate-independent pathway, in the biosynthesis of SDB and phytol was suggested, while b-sitosterol was indicated to be biosynthesized via the mevalonate pathway.26) Despite the fact that both SDB and phytol are biosynthesized from geranylgeranyl diphosphate (GGDP) via the DOXP pathway, the present results indicate the occurrence of different regulatory mechanisms in diterpene biosynthesis. This might be explained by a flux of GGDP toward SDB production due to MeJA treatment, although the actual mechanism involved in the process remains unclear.The time course of tissue growth and production of SDB by tissues was followed over a 10 d after treatment with 10 mM MeJA. The control tissues without MeJA increased steadily in growth during culture period, while MeJA suppressed tissue growth significantly 6 d after treatment (pϽ0.05) ( Fig. 2A). ...

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Selective Enhancement of Scopadulcic Acid B Production in the Cultured Tissues of Scoparia dulcis by Methyl Jasmonate

Nkembo

Lee

Hayashi

2005

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…In previous studies, Taxol concentrations were increased in Taxus suspension cultures by adding of MeJA to culture media (Sun et al 2001;Moon et al 1998). During Taxol biosynthesis, transcriptional expression of key enzyme genes was upregulated by MeJA (Walker and Croteau 2000;Walker et al 2002;Laskaris et al 1999). Currently, no transcription factor, which is involved in taxol biosynthesis, has been isolated from Taxus.…”

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confidence: 99%

Isolation and characterization of a novel cDNA encoding methyl jasmonate-responsive transcription factor TcAP2 from Taxus cuspidata

et al. 2009

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A novel transcription factor, TcAP2, was isolated from Taxus cuspidata by yeast one-hybrid strategy. This factor interacts with jasmonate- and elicitor-responsive element. Analysis of the deduced TcAP2 amino acid sequence revealed that TcAP2 contained a conserved AP2/ethylene-responsive element binding protein domain that consisted of 268 amino acids in a potential nuclear localization sequence. The factor of TcAP2 had a high homology, in its AP2 domain, to other AP2 family members. Based on phylogenetic analysis, it was different from other five DRE-binding proteins in their evolutionary relationship. The transcription of TcAP2 gene in yew accumulated primarily in young organs, such as young stems. Quantitative real-time RT-PCR analysis indicated that TcAP2 gene was inducible to express by treatments with methyl jasmonate plus salicylic acid, high salinity, and cold. This gene showed no response to either abscisic acid or drought treatment.

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“…Manipu lation with cultural medium composition in com bination with efficient selection allowed in a num ber of cases accumulation of paclitaxel in cells up to 0.03-0.05 % d. w. that is comparable or even surpasses the metabolite level in T. brevifolia bark [45,46]. Further investigation proved efficiency of elicitation for taxoid biosynthesis stimulation because a number of important enzymes of ter pene pathway (for instance geranylgeranyl diphos phate synthase and taxadiene synthase) are jas monate inducible [47,48].…”

Section: Recent Advances In Plant Biotechnology and Genetic Engineerimentioning

confidence: 99%

Recent advances in plant biotechnology and genetic engineering for production of secondary metabolites

Sheludko

2010

Cytol. Genet.

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Induction of geranylgeranyl diphosphate synthase activity and taxane accumulation in Taxus baccata cell cultures after elicitation by methyl jasmonate

Cited by 44 publications

References 20 publications

Selective Enhancement of Scopadulcic Acid B Production in the Cultured Tissues of Scoparia dulcis by Methyl Jasmonate

Selective Enhancement of Scopadulcic Acid B Production in the Cultured Tissues of Scoparia dulcis by Methyl Jasmonate

Isolation and characterization of a novel cDNA encoding methyl jasmonate-responsive transcription factor TcAP2 from Taxus cuspidata

Recent advances in plant biotechnology and genetic engineering for production of secondary metabolites

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