Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation

Mignolli, Francesco; Vidoz, María Laura; Mariotti, Lorenzo; Lombardi, Lara; Picciarelli, Piero

doi:10.1007/s10725-014-0002-1

Cited by 17 publications

(13 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…) is the result of upregulation imposed by the reduction of the GA20oxs activity. It is noteworthy that SlGA20ox1 expression is likely to be controlled by auxin and by its signalling components (Martí et al , Mignolli et al ). This supports the hypothesis that auxin‐induced GA biosynthesis is largely, yet not completely, regulated by DGT/Cyp1 through the action of SlGA20ox1 .…”

Section: Discussionmentioning

confidence: 99%

“…For total fruit RNA extraction, cDNA synthesis and quantitative polymerase chain reaction (qPCR) set up, we followed the method described by Mignolli et al (). In brief, frozen fruit tissues of approximately 0.1 g were ground in mortar with the addition of 1 ml of TRI Reagent® (MRC, Cincinnati, OH).…”

Section: Methodsmentioning

confidence: 99%

“…Endogenous GAs were determined in AC and dgt entire fruits following pollination and application of mock and 4‐CPA according to the methodology described by Mignolli et al (). In short, 1 g of frozen samples was ground in 80% methanol, centrifuged and the supernatant was collected.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Gibberellins modulate auxin responses during tomato (Solanum lycopersicum L.) fruit development

Mignolli

Vidoz

Picciarelli

et al. 2018

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

In tomato, auxin and gibberellins (GAs) interact with each other to drive fruit growth and development. While the role of auxin in directing GA biosynthesis and signal is already known, very little information has been obtained about GA-mediated control of auxin signalling and response. Interestingly, we show that gibberellic acid (GA ) is able to modify the expression of several auxin signalling genes in the partial auxin-insensitive diageotropica (dgt) mutant, suggesting that GAs may override the control of DGT on auxin signal. Procera (pro) mutation, which confers a constitutively active GA signal, enhances the effects of exogenous auxin, indicating that PRO may act as a negative effector of auxin responses in fruits. Indeed, transcript modulation of some auxin/indole acetic acid and auxin response factor genes in auxin-treated dgt/pro fruits suggests that PRO controls their expression possibly bypassing DGT. It was also shown that GA biosynthesis, in response to auxin treatment, is largely controlled by DGT. It is therefore conceivable that the DGT-mediated increase of active GAs in auxin-treated or pollinated fruits would promote PRO degradation, which in turn activates part of the auxin signalling cascade.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Gibberellins modulate auxin responses during tomato (Solanum lycopersicum L.) fruit development

Mignolli

Vidoz

Picciarelli

et al. 2018

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

show abstract

“…In tomato, parthenocarpic induction by auxin is also mediated at least partially by GA, as cotreatment with paclobutrazol (PAC; an inhibitor of GA biosynthesis) and auxin greatly reduces parthenocarpy, which can be reversed by coapplication with GA (Serrani et al, 2008). In addition, bioactive GA levels are elevated in auxin (treatment or iaa9/entire)induced parthenocarpic fruits due to increased expression of GA biosynthesis genes and reduced expression of GA catabolism genes (Serrani et al, 2008;Mignolli et al, 2015). Therefore, it was suggested that auxin induces fruit initiation in part through the induction of GA biosynthesis.…”

mentioning

confidence: 99%

The Interaction between DELLA and ARF/IAA Mediates Crosstalk between Gibberellin and Auxin Signaling to Control Fruit Initiation in Tomato

et al. 2018

View full text Add to dashboard Cite

Fruit initiation following fertilization in angiosperms is strictly regulated by phytohormones. In tomato (), auxin and gibberellin (GA) play central roles in promoting fruit initiation. Without fertilization, elevated GA or auxin signaling can induce parthenocarpy (seedless fruit production). The GA-signaling repressor SlDELLA and auxin-signaling components SlIAA9 and SlARF7 repress parthenocarpy, but the underlying mechanism is unknown. Here, we show that SlDELLA and the SlARF7/SlIAA9 complex mediate crosstalk between GA and auxin pathways to regulate fruit initiation. Yeast-two-hybrid and coimmunoprecipitation assays showed that SlARF7 and additional activator SlARFs interact with SlDELLA and SlIAA9 through distinct domains. SlARF7/SlIAA9 and SlDELLA antagonistically modulate the expression of feedback-regulated genes involved in GA and auxin metabolism, whereas SlARF7/SlIAA9 and SlDELLA coregulate the expression of fruit growth-related genes. Analysis of (), (with downregulated expression of multiple activator), and () single and double mutants indicated that these genes additively affect parthenocarpy, supporting the notion that the SlARFs/SlIAA9 and SlDELLA interaction plays an important role in regulating fruit initiation. Analysis of the GA-deficient mutant showed that active GA biosynthesis and signaling are required for auxin-induced fruit initiation. Our study reveals how direct crosstalk between auxin- and GA-signaling components is critical for tomato fruit initiation.

show abstract

“…Parthenocarpy induction in tomato by cytokinin depends in part on modulation of GA and indole-3-acetic acid (IAA) metabolism, as reflected by the upregulated expression levels of GA-biosynthesis genes such as GPS , GA20ox and GA3ox , and the IAA-biosynthesis gene ToFZY , and downregulation of the GA-inactivation gene GA2ox [ 16 ]. Auxin-induced parthenocarpic tomato fruit set is partially mediated by GAs, as indicated by the dependence of parthenocarpic fruit formation in the unfertilized entire tomato mutant with a defective SlIAA9 gene on regulation of GA metabolism [ 17 ]. SlIAA9 , a member of the Aux/IAA family, is an auxin-signaling repressor that prevents tomato ovary development [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis unravels spatiotemporal modulation of phytohormone-pathway expression underlying gibberellin-induced parthenocarpic fruit set in San Pedro-type fig (Ficus carica L.)

Chai

Chen

et al. 2018

BMC Plant Biol

View full text Add to dashboard Cite

BackgroundGibberellin (GA) treatments can induce parthenocarpy in the main crop of San Pedro-type figs, the native non-parthenocarpic fruit, however, the underlying mechanism is still largely unclear.ResultsIn our study, GA3 was applied to San Pedro-type fig main crop at anthesis. Sharply increased GA3 content was detected in both female flowers and receptacle, along with significantly decreased indole-3-acetic acid (IAA), zeatin and abscisic acid (ABA) levels in female flowers, and increased zeatin peak intensity and earlier ABA peak in receptacles. Transcriptome comparison between control and treatment groups identified more differentially expressed genes (DEGs) in receptacles than in female flowers 2 and 4 days after treatment (DAT); 10 DAT, the number of DEGs became similar in the two tissues. Synchronized changing trends of phytohormone-associated DEGs were observed in female flowers and receptacles with fruit development. Modulation of ethylene and GA signaling and auxin metabolism by exogenous GA3 occurred mainly 2 DAT, whereas changes in auxin, cytokinin and ABA signaling occurred mainly 10 DAT. Auxin-, ethylene- and ABA-metabolism and response pathways were largely regulated in the two tissues, mostly 2 and 10 DAT. The major components altering fig phytohormone metabolic and response patterns included downregulated GA2ox, BAS1, NCED and ACO, and upregulated ABA 8′-h and AUX/IAA.ConclusionsThus GA-induced parthenocarpy in fig is co-modulated by the female flowers and receptacle, and repression of ABA and ethylene biosynthesis and GA catabolism might be the main forces deflecting abscission and producing fig parthenocarpy.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1318-1) contains supplementary material, which is available to authorized users.

show abstract

Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation

Abstract: In tomato (Solanum lycopersicum L.

Cited by 17 publications

References 50 publications

Gibberellins modulate auxin responses during tomato (Solanum lycopersicum L.) fruit development

Gibberellins modulate auxin responses during tomato (Solanum lycopersicum L.) fruit development

The Interaction between DELLA and ARF/IAA Mediates Crosstalk between Gibberellin and Auxin Signaling to Control Fruit Initiation in Tomato

Transcriptome analysis unravels spatiotemporal modulation of phytohormone-pathway expression underlying gibberellin-induced parthenocarpic fruit set in San Pedro-type fig (Ficus carica L.)

Contact Info

Product

Resources

About