Induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes

Petrova, Maria; Zayova, Ely; Vlahova, M.

doi:10.2478/s11535-013-0157-6

Cited by 12 publications

(13 citation statements)

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“…The induction of hairy roots culture was described by Petrova et al (2013). The transgenic clone (T4) that showed the best growth characteristics was used to study the effects of sucrose, maltose, and glucose (added to the agar solidified Murashige and Skoog (1962) nutrient medium (MS) at concentrations of 1%, 3%, 5%, 7%, and 9%) on the accumulation of biomass and metabolite production of A. montana hairy roots.…”

Section: Plant Materialsmentioning

confidence: 99%

Influence of carbon sources on growth and GC-MS basedmetabolite profiling of Arnica montana L. hairy roots

Petrova¹,

Zayova²,

Dincheva³

et al. 2015

Turk J Biol

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Arnica montana L. (Asteraceae) is an economically important herb that contains numerous valuable biologically active compounds accumulated in various parts of the plant. The effects of carbon sources (sucrose, maltose, and glucose) at different concentrations (1%, 3%, 5%, 7%, and 9%) on growth were studied and GC-MS based metabolite profiling of A. montana hairy roots was conducted. The optimal growth and biomass accumulation of transformed roots were observed on an MS nutrient medium containing 3% or 5% sucrose. GC-MS analysis of hairy roots of A. montana showed the presence of 48 compounds in polar fractions and 22 compounds in apolar fractions belonging to different classes of metabolites: flavones, phenolic acids, organic acids, fatty acids, amino acids, sugars, sugar alcohols, hydrocarbons etc. Among the various metabolites identified, only the sugars and sugar alcohols were influenced by the concentration of the respective carbon sources in the nutrient medium.

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Section: Plant Materialsmentioning

confidence: 99%

Influence of carbon sources on growth and GC-MS basedmetabolite profiling of Arnica montana L. hairy roots

Petrova¹,

Zayova²,

Dincheva³

et al. 2015

Turk J Biol

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“…The infection time influenced the frequency of HR formation. Petrova et al, (2013) reported increased transformation frequency as the infection time increased from 5 to 20 minutes. Short infection time may result in low availability of bacteria for transforming the plant cells while long durations may lead to a decline in the transformation rate by competitive inhibition (Kumar et al, 1991).…”

Section: Effect Of Concentrations Of Bacterial Suspension and Times O...mentioning

confidence: 95%

Investigation of factors in improving Agrobacterium-mediated gene transfer in Ruellia tuberosa L. and evaluation of α-glucosidase inhibitory activity in established hairy roots

Cao

TRAN

Quach

2022

Not Bot Horti Agrobo

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Ruellia tuberosa (family Acanthaceae) is widely known in traditional medicine in Asian countries for the treatment of diabetes and other diseases. Its roots were demonstrated to possess a hypoglycemic ability in diabetic animal models. In this study, an original induced procedure was investigated to establish hairy root (HR) from R. tuberosa. With the aim of increasing the transformation rate, some induced factors (acetosyringone (AS) dosage, type of explant, age, infection time, bacterial density, co-cultivation duration) were individually examined. As a result, an improved procedure was implemented: ten-day-old in vitro cotyledon explants were injured and then immersed in the bacterial suspension (OD600 nm = 0.4) added 200 µM AS during 10 min. The infected explants were co-cultivated for 4 days in the Murashige & Skoog (MS) medium before transferring to the medium containing cefotaxime for bacterial elimination. After thirty days of culture, the improved procedure revealed a synergistic effect by enhancing the rooting rate and number of secondary roots per explant up to 4.4- and 8.0-fold, respectively, in comparison with the original procedure. The R. tuberosa HR was then cultured in liquid MS medium and achieved the highest biomass production at the late exponential growth phase (3rd week). Its ethanol extract was also higher 2.0-fold in α-glucosidase inhibitory activity than that of the natural root. In conclusion, the α-glucosidase inhibitory activity of HR inducing by the improved procedure may offer an effective and reliable substitute for the utilization of this herbal plant.

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“…Trong nuôi cấy rễ tơ ở hầu hết các loài thực vật nói chung, sucrose được sử dụng như nguồn carbon thích hợp nhất để thu nhận sinh khối. Thí dụ như dòng rễ tơ T4 từ cây Arnica montana L. cho lượng sinh khối tươi cao nhất trên môi trường có sucrose [18]. Tuy nhiên, trong vài trường hợp, các loại đường khác cũng cho chỉ số tăng trưởng tương đương với sucrose như dòng rễ tơ 14-P từ cây Picrorhiza kurroa [30].…”

Section: Sự Phát Triển Của Rễ Tơ ở Môi Trường Có Nguồn Carbon Khác Nhauunclassified

“…Tuy nhiên, có những dòng rễ tơ có thể phát triển tốt trong một dãy nồng độ sucrose khác nhau. Thí dụ như dòng rễ tơ T4 cây Arnica montana L. tăng trưởng cao nhất ở môi trường có sucrose 3-5% [18] hay như sự phát triển của dòng rễ tơ số 8 từ cây dừa cạn cũng không có sự khác biệt đáng kể giữa trong dãy nồng độ sucrose này [26]. Qua đó có thể thấy nồng độ sucrose có ảnh hưởng khác nhau tùy từng dòng rễ tơ và không phụ thuộc vào loài thực vật.…”

Section: Sự Phát Triển Của Rễ Tơ ở Môi Trường Có Nồng độ Sucrose Khácunclassified

Study on the growth of Agrobacterium rhizogenes-induced Catharanthus roseus hairy roots in liquid media

Nhứt¹,

Lệ²

2019

Sci. Tech. Dev. J. - Nat. Sci.

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Catharanthus roseus hairy roots induced by Agrobacterium rhizogenes get potential to apply to many fields. The suitable conditions for culturing depend on each hairy root line. In shaken liquid media in dark at 25 oC, VIN002-12 and VIN005-07 hairy root lines had the best growth in hemi-concentrated Gamborg’B5 media while VIN072-15 and VIN077-09 lines showed the best growth in in hemi-concentrated White media. The appropriate initial pH of medium for the lines was in range of 5.7–6.5. The hairy root lines used 2–5% sucrose as an optimal source of carbon for their growth. In selected conditions, the growth kinetics curves showed the end of exponential phase at the 28th day of culture with VIN005-07 and VIN072- 15 lines whereas at the 35th day with VIN002-12 and VIN077-09 lines. The initial results are quite possible to produce biomass for researches on four hairy root lines in the future.

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Induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes

Abstract: Abbreviations:MS -Murashige and Skoog medium; B5 -Gamborg's medium; SH -Schenk and Hildebrandt medium; PCR -polymerase chain reaction; MP -micropropagated plants; RP -regenerated plants from callus culture.

Cited by 12 publications

References 58 publications

Influence of carbon sources on growth and GC-MS basedmetabolite profiling of Arnica montana L. hairy roots

Influence of carbon sources on growth and GC-MS basedmetabolite profiling of Arnica montana L. hairy roots

Investigation of factors in improving Agrobacterium-mediated gene transfer in Ruellia tuberosa L. and evaluation of α-glucosidase inhibitory activity in established hairy roots

Study on the growth of Agrobacterium rhizogenes-induced Catharanthus roseus hairy roots in liquid media

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