Induction of Haploid Rice Plant from Anther Culture

Niizeki, Hiroo; Oono, Kiyoharu

doi:10.2183/pjab1945.44.554

Cited by 250 publications

(75 citation statements)

References 7 publications

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“…Among the factors influencing the response, genetic factors are an important determinant of the level of response (Chen 1988;Henry et al 1994). Many authors have reported that considerable variation among genotypes has been identified (Niizeki and Oono 1968;Mukherjee 1973;Oono 1975;Shen et al 1982;Chen 1988;Li 1991). Moreover, previous studies also showed that anther culture capacity in rice was highly heritable and was controlled by nuclear genes (Miah et al 1985;Quimio and Zapata 1990).…”

Section: Introductionmentioning

confidence: 99%

Selection of Interspecific Hybrids for Anther Culture of Indica Rice

Yang²,

et al. 2006

Plant Cell Tiss Organ Cult

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The low percentage of callus induction and green-plant regeneration has limited the application of anther culture techniques in indica rice breeding programmes. The aim of this work was to investigate the inheritance of the two characters and to determine whether good combinations with high-frequency callus induction and green-plant regeneration could be selected by sexual hybridization of suitable parents. So, a 3 · 4 incomplete diallel crosses were conducted between 7 parental lines. Three of them were believed to possess relatively high callus induction capacity and the other four relatively high green-plant regeneration capacity, based on our previous studies. Analysis of combining abilities and heritabilities revealed that both additive and non-additive effects were found to be important in the genetic control of callus induction. In case of green-plant regeneration, non-additive effects were predominant. Broad sense heritability values of 0.92 for callus induction and 0.98 for greenplant regeneration suggested that more than 90% of the observed variation was due to genetic effects, with just less than 10% attributed to nongenetic or environmental effects. In addition, the moderate narrow sense heritability values of 0.49 for callus induction, 0.29 for green-plant regeneration showed that the potential to introduce high responding ability into low lines was moderate. It was therefore conceivable that the frequency of anther culture response might be improved by suitable hybridization and selection strategies. A hybrid combination of code no. 67/ 107 incorporating high callus induction and high green-plant regeneration ability was selected in which genotype with high values of general combining ability (GCA) and positive effects of specific combining ability (SCA).

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Section: Introductionmentioning

confidence: 99%

Selection of Interspecific Hybrids for Anther Culture of Indica Rice

Yang²,

et al. 2006

Plant Cell Tiss Organ Cult

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“…The anther culture technique, first developed in rice by Niizeki and Oono (1968), allows to obtain stabilized double haploid (DH) plants bypassing the inbreeding process. Moreover, it is the fastest method to obtain DH rice plants as can be performed in less than one year (Agache et al 1989;Miah et al 1985).…”

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confidence: 99%

An improved anther culture procedure for obtaining new commercial Mediterranean temperate <i>japonica</i> rice (<i>Oryza sativa</i>) genotypes

López-Cristoffanini

Serrat

Ramos-Fuentes

et al. 2018

Plant Biotechnology

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Rice is one of the greatest calorie supply for the world population, especially since its production is almost entirely destined to direct human consumption and its demand will increase along with the world population. There are efforts worldwide to increase rice yields by obtaining new improved and stabilized rice lines. The rice anther culture, a fast and cheap technique, allows to obtain double haploid lines in less than one year. We report its application with an improved protocol in four Mediterranean japonica rice genotypes at F 2 generation. We performed a screening test for cold-pretreatment at 5.0±0.1°C and concluded that the optimum duration was 9 days as it produced the higher rate of anther-derived callus induction. This revised protocol was successfully applied to the four genotypes, obtaining good results in all the procedure's steps. At the end, more than 100 of double haploid green plants were generated. Moreover, 9 lines obtained from the anther culture procedure showed good qualities for the Spanish market at the growing, farming and grain production level during the field assays. Therefore, we report an improved anther culture procedure for obtaining double haploid lines from temperate japonica rice genotypes showing high commercialization expectance.

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“…NITSCH et ses collaborateurs (1967,1968,1969) ont ainsi realise des haploides de Nicotiana; puis, NAKATA et TANAKA (1968} ont reussi eux aussi a regenerer des plantes haploides de Nicotiana tabacum; la meme annee, NIIZEKI et OoNO ( 1968) sont parvenus a regenerer des plantules haploides a partir de cals provenant de cultures d'antheres de riz.…”

Section: Introductionunclassified