Induction of heat shock proteins and their implication in protection against ethanol-induced damage in cultured guinea pig gastric mucosal cells

Nakamura, Kunihide; Rokutan, Kazuhito; Marui, Nobuyuki; Aoike, Akira; Kawai, Keiichi

doi:10.1016/0016-5085(91)90473-x

Cited by 141 publications

(108 citation statements)

References 25 publications

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“…Indeed, the induction of HSPs by hyperthermia (Nakamura et al 1991), geranylgeranylacetone (Hirakawa et al 1996), and aspirin (Jin et al 1999) could protect gastric mucosa against necrotizing stimuli in experimental animals. Whether or not CBX can induce HSPs in gastric mucosa has not been examined yet, the cytoprotective effect of CBX might be partly due to its chaperone inducing activity.…”

Section: Discussionmentioning

confidence: 99%

Reinvestigation of the effect of carbenoxolone on the induction of heat shock proteins

Kawashima

Asai

Katagiri

et al. 2009

Cell Stress and Chaperones

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Carbenoxolone (CBX) is a semisynthetic derivative of the licorice root substance glycyrrhizinic acid and has been previously reported to induce only heat shock protein 70 [Hsp70, HSPA1A (the systematic name of heat shock protein is given in the parenthesis after each HSP, according to the recent nomenclature guidelines, Kampinga et al., Cell Stress Chaperones, 14:105-111, 2008) but not other heat shock proteins (HSPs) (Nagayama et al., Life Sci. 69:2867-2873. In this study, we reinvestigated the effect of CBX on the induction of HSPs in HeLa and human neuroblastoma (A-172) cells. CBX clearly induced not only Hsp70 but also Hsp90 (HSPC1), Hsp40 (DNAJB1), and Hsp27 (HSPB1) at concentrations of 10 to 800 μM for 16 h incubation. At higher concentrations (more than 400 μM), however, CBX appeared to be toxic. Treatment of cells with CBX resulted in enhanced phosphorylation and acquisition of DNA-binding ability of heat shock transcription factor 1 (HSF1). Furthermore, characteristic HSF1 granules were formed in the nucleus, suggesting that the induction of HSPs by CBX is mediated by the activation of HSF1. Furthermore, thermotolerance was induced by CBX treatment, as determined by clonogenic survival. Although the precise target of CBX is not known at present, these results indicate that CBX is one of the molecular chaperone inducers and suggest that some pharmacological activities of CBX might be ascribable in part to its molecular chaperone-inducing property.

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Section: Discussionmentioning

confidence: 99%

Reinvestigation of the effect of carbenoxolone on the induction of heat shock proteins

Kawashima

Asai

Katagiri

et al. 2009

Cell Stress and Chaperones

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“…HSPs may also play a cytoprotective role in gastrointestinal tissue. For example, guinea-pig gastric mucosal cells in which HSPs were induced by heat-shock treatment showed considerable resistance against injury induced by 7.5% ethanol [33]. In addition, oral administration of geranylgeranylacetone (GGA), an anti-ulcer drug, rapidly induced HSP60, heatshock cognate protein 70, HSC70, and HSP90 in rat gastric mucosal cells, and these induced HSPs contributed to the suppression of ulcer formation induced by water-immersion restraint stress [34].…”

Section: Discussionmentioning

confidence: 99%

Ginseng, the Root of Panax ginseng C.A. Meyer, Protects Ethanol-Induced Gastric Damages in Rat through the Induction of Cytoprotective Heat-Shock Protein 27

et al. 2007

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Ginseng, the root of Panax ginseng C.A. Meyer, has been reported to exert preventive effects on gastropathy via anti-oxidative and anti-inflammatory actions. In this study, we investigated the protective effects of ginseng against ethanol-induced gastric damages in rat. To examine the preventive effect of ginseng, rats received two different ginseng extracts, A and B, 1 h prior to the administration of ethanol. Pretreatment of rats with ginseng extract A and B attenuated the ethanol-induced gastric lesions by 111 AE 48 and 142 AE 47 mm 2 compared to control group (164 AE 54 mm 2 ). Significant induction of cytoprotective heat-shock proteins HSP27 and HSP70 was found in the ginseng-administrated rats, suggesting that the restoration of the proteins might contribute to prevention of ethanol-induced gastric injuries. It is, therefore, suggested that ginseng has a protective effect against ethanol-induced gastric damages by induction of heat-shock proteins 70 and 27.

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“…Pronase I (Kaken Pharmaceutical, Tokyo), collagenase S-1 from Streptomyces parvulus (Nitta Gelatin, Osaka), and bovine serum albumin fraction V (Serologicals Proteins, IL, U.S.A.) were used for digestive treatment of fundic glands. 21) Purified Helicobacter pylori lipopolysaccharide (LPS) 18,22) was stocked in Ca…”

Section: Methodsmentioning

confidence: 99%

“…GMC were obtained by primary culture of gastric epithelial cells isolated from fundic glands of Hartley clean guinea pig stomachs, as previously described. 18,21) Cells were cultured in RPMI 1640 medium containing 2 mM L-glutamine, 10% (v/v) FBS, 0.1 mg/ml streptomycin, and 100 U/ml penicillin under 5% CO 2 /95% air at 37°C, with daily medium changes until harvest. On day 2, when the cell population consists in its majority (Ͼ90%) of gastric pit cells, 18) purified H. pylori LPS was added to the medium (5 ng/ml) and culture was continued for a further 24 h before harvest, as follows: cells were washed 2-3 times with PBS, detached from plates by incubation with warm 0.02% (w/v) trypsin-and 1.5 mM EDTAcontaining PBS for 2 min at 37°C, and immediately diluted in medium with 5% (v/v) FBS.…”

Section: ϩ2mentioning

confidence: 99%

Superoxide Generation by Nox1 in Guinea Pig Gastric Mucosal Cells Involves a Component with p67phox-Ability

Yoshida

Nishida

Shimoyama

et al. 2004

Biological & Pharmaceutical Bulletin

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Induction of heat shock proteins and their implication in protection against ethanol-induced damage in cultured guinea pig gastric mucosal cells

Cited by 141 publications

References 25 publications

Reinvestigation of the effect of carbenoxolone on the induction of heat shock proteins

Reinvestigation of the effect of carbenoxolone on the induction of heat shock proteins

Ginseng, the Root of Panax ginseng C.A. Meyer, Protects Ethanol-Induced Gastric Damages in Rat through the Induction of Cytoprotective Heat-Shock Protein 27

Superoxide Generation by Nox1 in Guinea Pig Gastric Mucosal Cells Involves a Component with p67phox-Ability

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