Induction of heat-shock (stress) protein gene expression by selected natural and anthropogenic disturbances in the octocoral Dendronephthya klunzingeri

Wiens, Matthias; Ammar, Mohammed S. A.; Nawar, Ahmed H; Koziol, Claudia; Hassanein, Hamdy; Eisinger, Michael; Müller, Isabel M.; Müller, Werner

doi:10.1016/s0022-0981(99)00167-7

Cited by 41 publications

(23 citation statements)

References 39 publications

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“…HSP 70 and 90 expression has been previously reported in cnidarians by others using the same or similar techniques (Miller et al 1992;Sharp et al 1994;Black et al 1995;Fang et al 1997;Sharp et al 1997;Wiens et al 2000;Rossi and Snyder 2001;Snyder and Rossi 2004).…”

Section: Hsp 70 and Hsp 90 Measurementsmentioning

confidence: 52%

Protein, carbohydrate, lipid concentrations and HSP 70–HSP 90 (stress protein) expression over an annual cycle: useful tools to detect feeding constraints in a benthic suspension feeder

2005

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Protein, carbohydrate, lipid concentrations and HSP 70-HSP 90 (stress protein) expression over an annual cycle: useful tools to detect feeding constraints in a benthic suspension feeder Abstract In the present paper we suggest an effect of seasonal variations in food availability on two ecophysiological parameters in a warm temperate benthic suspension feeder: the tissue concentrations of proteins, carbohydrates and lipids on the one hand, and the expression of stress proteins (HSP 70 and 90, inducible and/or constitutive) on the other hand. The concentrations of biomacromolecules have already been used to describe bentho-pelagic and reproductive processes, but this is the first time that stress protein expression is suggested to be directly related with food constraints in marine organisms. Paramuricea clavata (Cnidaria: Gorgonacea) express HSP 70 and 90 (constitutive and/ or inducible) throughout the seasonal cycle, and HSP 70 levels are twice as high as the levels of HSP 90. In summer and autumn, when seston availability to suspension feeders was low, P. clavata showed low levels of carbohydrates and lipids, but high levels of HSPs expression. The levels of HSP 70 and 90 expression fit with negative exponential functions of carbohydrate and lipid concentrations. We suggest a direct effect of food availability on the studied ecophysiological parameters while the effect of temperature may be rather indirect. HSP expression as well as the tissue concentrations of carbohydrate and lipids may be used as biomarkers of environmental changes and seston availability to benthic suspension feeders.

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Section: Hsp 70 and Hsp 90 Measurementsmentioning

confidence: 52%

Protein, carbohydrate, lipid concentrations and HSP 70–HSP 90 (stress protein) expression over an annual cycle: useful tools to detect feeding constraints in a benthic suspension feeder

2005

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“…Hence, the HSP90 family provides a useful model system for evolutionary studies (Gupta 1995). In addition, the response of these proteins to environmental stresses, such as changes in temperature (Palmisano et al 2000;Hermesz et al 2001;Landais et al 2001), hyperosmotic shock (Pan et al 2000), hypoxia (Li et al 2008), metal ions (Ali et al 1996), polychlorinated biphenyls (Wiens et al 2000), and also diseases (Ramaglia et al 2004) has been examined. Despite of these various studies, only five complete cDNA sequences have been reported from mollusks (Farcy et al 2007;Gunter and Degnan 2007), including three HSP90 cDNA sequences in bivalves (Gao et al 2007(Gao et al , 2008Choi et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and induction of heat shock protein 90 in the Antarctic bivalve Laternula elliptica

Kim

Ahn

Kim

et al. 2009

Cell Stress and Chaperones

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Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone that plays a key role in protein synthesis, folding, denaturation prevention, and signal transduction. We cloned the complete complementary DNA (cDNA) sequence of the Laternula elliptica HSP90. The full-length cDNA was 2,823 bp in size and contained an open reading frame of 2,190 bp that was translated into 729 amino acids with a calculated molecular weight of 83.4 kDa. The deduced amino acid sequence of HSP90 showed the highest homology to Haliotis tuberculata HSP90 (83%). Reverse-transcriptase polymerase chain reaction analysis revealed the presence of HSP90 transcripts in all of the tissues examined. We also studied the transcriptional expression pattern of HSP90 exposed to thermal stress with real-time polymerase chain reaction. The relative expression level of HSP90 messenger RNA was upregulated and peaked at 12 h in the digestive gland and at 24 h in the gills, then dropped progressively.

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“…Corals under stress respond by producing stress proteins, also called heat shock proteins. These are used as biomarkers to monitor environmental stress in corals (Weins et al 2000). The increased production of stress proteins may also change the RNA/DNA ratios in stressed corals.…”

Section: Introductionmentioning

confidence: 99%

Stress response of two coral species in the Kavaratti atoll of the Lakshadweep Archipelago, India

2005

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Frequent occurrences of coral bleaching and the ensuing damage to coral reefs have generated interest in documenting stress responses that precede bleaching.. The objective of this study was to assess and compare physiological changes in healthy, semi-bleached and totally bleached colonies of two coral species, Porites lutea and Acropora formosa during a natural bleaching event in the Lakshadweep Archipelago in the Arabian Sea to determine the traits that will be useful in diagnosis of coral health. In April, 2002, three "health conditions" were observed as "appearing healthy", "semi-bleached" and "bleached" specimens for two dominant and co-occurring coral species in these islands. Changes in the pigment composition, zooxanthellae density, mitotic index of zooxanthellae, RNA/DNA ratios and protein profile in the two coral species showing different levels of bleaching in the field were compared to address the hypothesis of no difference in health condition between species and bleaching status. The loss in chlorophyll a and chlorophyll c and zooxanthellae density in the transitional stage of semi-bleaching in the branched coral A. formosa was 80, 75 and 80% respectively. The losses were much less in the massive coral P. lutea, being 20, 50 and 25% respectively. The decrease in zooxanthellar density and chlorophyll a was accompanied by an increased mitotic index of zooxanthellae and RNA/DNA ratios in both the species. There was an increase in accumulation of lipofuscin granules in partially bleached P. lutea tissue, which is an indication of cellular senescence. Multivariate statistical analyses showed that colonies of P. lutea ranked in different health conditions differed significantly in chl a, chl c, zooxanthellae density, RNA/DNA ratios and protein concentrations whereas in A. formosa chl a, chl c, chl a/c, phaeopigments and mitotic index contributed to the variance between health conditions.

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Induction of heat-shock (stress) protein gene expression by selected natural and anthropogenic disturbances in the octocoral Dendronephthya klunzingeri

Cited by 41 publications

References 39 publications

Protein, carbohydrate, lipid concentrations and HSP 70–HSP 90 (stress protein) expression over an annual cycle: useful tools to detect feeding constraints in a benthic suspension feeder

Protein, carbohydrate, lipid concentrations and HSP 70–HSP 90 (stress protein) expression over an annual cycle: useful tools to detect feeding constraints in a benthic suspension feeder

Molecular characterization and induction of heat shock protein 90 in the Antarctic bivalve Laternula elliptica

Stress response of two coral species in the Kavaratti atoll of the Lakshadweep Archipelago, India

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