Induction of HLA Class I-Restricted CD8+ CTLs Specific for the Major Outer Membrane Protein of <i>Chlamydia trachomatis</i> in Human Genital Tract Infections

Kim, Seon Kyeong; Angevine, Mark; Demick, Karen P.; Ortiz, Linette; Rudersdorf, Richard; Watkins, David I.; DeMars, Robert

doi:10.4049/jimmunol.162.11.6855

Cited by 65 publications

(1 citation statement)

References 54 publications

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“…Several CD+ T-cell antigens are known in Chlamydiae: MOMP [10], the 60-kDa cysteine-rich OMP protein (OmcB, Omc2, or Omc2B; CT443), PmpI (CT874) [12], cysteine-rich protein A (CrpA, CT442) [13], and class I accessible protein 1 (Cap1, CT529) [14]. The last two proteins occur on the membrane of chlamydial inclusions (table) and act as immunodominant antigens, which interact with a substantial number of sera from patients infected with C. trachomatis.…”

Section: The Interaction With the Immune System Of The Host Organismmentioning

confidence: 99%

Effector proteins of chlamydiae

et al. 2009

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This review summarizes the recently published data on the molecular mechanisms of Chlamydiaehost cell interaction, first of all, on chlamydial effector proteins. Such proteins, along with type III transport system proteins, which transfer many effector proteins into the host cytoplasm, are attractive targets for drug therapy of chlamydial infections. The majority of the data concerns two species, Chlamydia trachomatis and Chlamydophila pneumoniae. The C. trachomatis protein TARP, which is presynthesized in elementary bodies, plays an essential role in the initial stages of infection. The pathogen proteins that are involved in the next stage, which is the intracellular inclusion traffic to the centrosome, are C. trachomatis CT229 and C. pneumoniae Cpn0585, which interact with cell Rab GTPases. In C. trachomatis , IncA plays a key role in the fusion of chlamydial inclusions, CT847 modulates the life cycle of the host cell, and LDA3 is essential for the acquisition of nutrients. The protease CPAF and the inclusion membrane proteins IncG and CADD are involved in suppressing apoptosis of infected cells. The proteases CPAF and CT441 and the deubiquitinating protein ChlaDub1 help the pathogen to evade the immune response.

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Section: The Interaction With the Immune System Of The Host Organismmentioning

confidence: 99%

Effector proteins of chlamydiae

et al. 2009

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Analysis of the antigen-specific T cell response in reactive arthritis by flow cytometry

Thiel

Lauster

et al. 2000

Arthritis & Rheumatism

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Objective In reactive arthritis (ReA) a bacteria‐specific T cell response to the triggering microbe is detected in synovial fluid (SF), and an impaired Th1 cytokine response has been described. The recent identification of immunodominant bacterial proteins/peptides and new technologies make a more detailed analysis of the immune response possible. The aim of the present study was to use these new techniques to determine the antigen‐specific T cell frequency and the cytokine secretion pattern on stimulation with bacteria‐derived recombinant proteins in the peripheral blood (PB) and SF from patients with ReA. Methods In 3 patients with Chlamydia‐induced ReA and 2 patients with Yersinia‐induced ReA, the SF T cell response was investigated after stimulation with the Chlamydia‐derived proteins major outer membrane protein (MOMP) and heat‐shock protein 60 (Hsp60) and the Yersinia‐derived proteins 19‐kd protein and Hsp60. In 3 of these patients, the PB T cell response was investigated in parallel. T cells were stimulated in whole blood or whole SF with antigen plus anti‐CD28 for 6 hours, brefeldin A was added after 2 hours, and cells were fixed and stained with antibodies against the surface markers CD4 and CD69 and against the cytokines interferon‐γ (IFNγ), tumor necrosis factor α, interleukin‐10 (IL‐10), and IL‐4. Positive cells were quantified by flow cytometry. Results In the 3 patients with Chlamydia‐induced ReA, the antigen‐specific T cell frequency (percentage of IFNγ CD69 double‐positive CD4+ T cells) in response to MOMP (mean ± SD 1.2 ± 1.38%) and to Hsp60 (1.21 ± 1.45%) in SF was about the same. In the 2 patients with Yersinia‐induced ReA, the mean ± SD frequency was 0.66 ± 0.36% in response to the Hsp60 and 0.3% ± 0.22 in response to the 19‐kd protein. In the 3 patients whose PB was evaluated, the corresponding T cell response was ≥10 times lower. In 2 patients with Chlamydia‐induced ReA, antigen‐specific IL‐10–positive CD4+ T cells were detected in 0.10–0.23% of the CD4+ T cell subpopulation. Conclusion The frequency of antigen‐specific T cells to Chlamydia‐ and Yersinia‐derived antigens in the SF of ReA patients is between 1:200 and 1:50. Both the chlamydial Hsp60 and MOMP are dominant T cell antigens in Chlamydia‐induced ReA. In patients with Chlamydia‐induced ReA, we detected antigen‐specific IL‐10 secretion, which might mediate an inhibition of effective bacterial clearance.

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Vaccines Against Chlamydia pneumoniae: Can They Be Made?

Rottenberg

Rothfuchs

Wigzell

Infectious Agents and Pathogenesis

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Induction of HLA Class I-Restricted CD8+ CTLs Specific for the Major Outer Membrane Protein of Chlamydia trachomatis in Human Genital Tract Infections

Cited by 65 publications

References 54 publications

Effector proteins of chlamydiae

Effector proteins of chlamydiae

Analysis of the antigen-specific T cell response in reactive arthritis by flow cytometry

Vaccines Against Chlamydia pneumoniae: Can They Be Made?

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