Induction of IBV strain-specific neutralizing antibodies and broad spectrum protection in layer pullets primed with IBV Massachusetts (Mass) and 793B vaccines prior to injection of inactivated vaccine containing Mass antigen

Wit, Janneke; Malo, Aris; Cook, Jane K.A.

doi:10.1080/03079457.2018.1556778

Cited by 24 publications

(27 citation statements)

References 42 publications

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“…Also, such a combined approach in IB vaccination could also explored in the control of emerging IBVs and other coronaviruses variants [ 40 ]. Overall, our findings on the immunogenic potentials of the DNA vaccine is consistent with other studies who demostrated good immunogenic response and good protection following immunization with a vaccine containing the combinations of Massachussets and other IBV strains such as the 793B [ 41 , 42 ] or QX-like and Dutch strains [ 40 ].…”

Section: Discussionsupporting

confidence: 91%

“…Similarly, another study reported that a DNA plasmid encoding S1 gene resulted in 75% protection in challenged chickens, thus further demostrating the immunogenic potentials of IBV S1 glycoprotein [ 39 ]. The protection observed in this study interms of reduced virus sheddings, low kidney and tracheal lesion scores as well as reduced clinical signs have been reported in other similar studies [ 40 , 41 ], and might not be unconnected to the numerous immunogenic epitopes situated within the S1-glycoprotein of the two virus strains [ 29 ]. Also, such a combined approach in IB vaccination could also explored in the control of emerging IBVs and other coronaviruses variants [ 40 ].…”

Section: Discussionsupporting

confidence: 87%

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Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus

Bande

Arshad

Bejo

et al. 2020

Microbial Pathogenesis

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Infectious bronchitis (IB) is an economically important disease of poultry that also serve as model for the understanding of other coronaviruses associated diseases. IB is considered as a major challenge to the poultry industry worldwide as a result of its effect on egg production, weight gain as well as mortality. Different IBV genotypes continue to emerge, thus, the need for broad based vaccines to curb the disease. Based on bioinformatic data obtained in this study, sets of monovalent (either M41 or CR88) and bivalent DNA vaccines encoding the S1 glycoprotein from two different strains namely, M41 and CR88 were developed. The candidate vaccine was further encapsulated with a chitosan-saponin nanoparticle with the view to enhance its immunogenicity. Following in vitro characterization of the constructs, the vaccine candidates were tested in specific pathogen free (SPF) chickens. Analysis of humoral responses revealed a significant increase in anti-IBV antibody after immunization with the bivalent DNA plasmid (pBudCR88-S1/M41-S1). Likewise, cell mediated immune (CMI) response was significantly higher in vaccinated groups as compared to the unvaccinated chickens. Vaccinated chickens exhibited milder clinical signs as well as tracheal and kidney lesion scores following virus challenge as compared to the control groups. Additionally, encapsulation of the bivalent DNA vaccine with chitosan-saponin nanoparticles was found to improve protection against challenge with IBV strains M41 and CR88 as revealed by a significant reduction (p < 0.05) in oropharyngeal and cloacal virus sheddings following challenges with each of the two viruses. In contrast, monovalent IB-DNA vaccines were protective against homologous virus challenge. Moreover, nanoencasulation of the candidate vaccine was demostrated to abrogate the need for booster vaccination. In conclusion, this study demonstrates the immunogenic potentials of a bivalent IB-DNA vaccine and the use of chitosan-saponin nanoparticle to enhance its response. This development could serve as alternative strategy for the control of IB in poultry.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus

Bande

Arshad

Bejo

et al. 2020

Microbial Pathogenesis

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“…As a member of the genus Gammacoronavirus, IBV has a remarkably high mutation and recombination rate, leading to numerous types and variants that differ from each other in pathogenicity [2,3]. Although the site of entry of IBV is the upper respiratory tract, where the initial infection occurs, the virus can spread systemically, replicating in the epithelial cells of many organs and causing injuries of the kidneys and female reproductive tract [4]. The kidney, trachea, caecal tonsil, and cloaca have been demonstrated to be tissues in which the long-term persistence of IBV is observed [5].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the reproductive system development and egg-laying performance of hens infected with TW I-type infectious bronchitis virus

Zhang

Liao

Chen

et al. 2020

Vet Res

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The prevalence of TW I-type infectious bronchitis virus (IBV) has been increasing rapidly, and it has become the second most common genotype of IBV in China threatening the poultry industry. In this study, 1-day-old specific-pathogen-free (SPF) chickens infected with TW I-type IBV were continuously observed for 200 days. TW I-type IBV affected the respiratory, urinary, and female reproductive systems, resulting in a mortality rate of 10% as well as a decrease in egg quantity and an increase in inferior eggs. During the monitoring period, serious lesions occurred in the female reproductive system, such as yolk peritonitis, a shortened oviduct, and cysts of different sizes with effusion in the degenerated right oviduct. The infective viruses persisted in vivo for a long time, and due to the stress of laying, virus shedding was detected again after the onset of egg production. Our findings suggest that TW I-type IBV is deadly to chickens and could cause permanent damage to the oviduct, resulting in the poor laying performance of female survivors and decreasing the breeding value and welfare of the infected flock.

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“…Our results showed that HY could inhibit IBV infection and inhibit apoptosis and production of ROS in CEK cells. It has been reported that there were multiple known strains of IBV, IBV strain M41, is typical respiratory virus, which replicates primarily in the respiratory tract and subsequently spreads and replicates in a range of other tissues (De Wit et al, 2019;Hodgson et al, 2004). The CEK cells are the most susceptible cells of strain M41 in vitro (Abdel-Moneim et al, 2009;Zhang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Protective effects of hypericin against infectious bronchitis virus induced apoptosis and reactive oxygen species in chicken embryo kidney cells

et al. 2019

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Avian infectious bronchitis virus (IBV), a coronavirus, causes infectious bronchitis leading to enormous economic loss in the poultry industry worldwide. Hypericin (HY) is an excellent compound that has been investigated in antiviral, antineoplastic, and antidepressant. To investigate the inhibition effect of HY on IBV infection in chicken embryo kidney (CEK) cells, 3 different experimental designs: pre-treatment of cells prior to IBV infection, direct treatment of IBV-infected cells, and pre-treatment of IBV prior to cell infection were used. Quantitative real-time PCR (qRT-PCR), immunofluorescence assay (IFA), flow cytometry, and fluorescence microscopy were performed and virus titer was determined by TCID 50 . The re-sults revealed that HY had a good anti-IBV effect when HY directly treated the IBV-infected cells, and virus infectivity decreased in a dose-dependent manner. Furthermore, HY inhibited IBV-induced apoptosis in CEK cells, and significantly reduced the mRNA expression levels of Fas, FasL, JNK, Bax, Caspase 3, and Caspase 8, and significantly increased Bcl-2 mRNA expression level in CEK cells. In addition, HY treatment could decrease IBV-induced reactive oxygen species (ROS) generation in CEK cells. These results suggested that HY showed potential antiviral activities against IBV infection involving the inhibition of apoptosis and ROS generation in CEK cells.

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Induction of IBV strain-specific neutralizing antibodies and broad spectrum protection in layer pullets primed with IBV Massachusetts (Mass) and 793B vaccines prior to injection of inactivated vaccine containing Mass antigen

Cited by 24 publications

References 42 publications

Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus

Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus

Evaluation of the reproductive system development and egg-laying performance of hens infected with TW I-type infectious bronchitis virus

Protective effects of hypericin against infectious bronchitis virus induced apoptosis and reactive oxygen species in chicken embryo kidney cells

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