Induction of Influenza A Virus Cross-reactive Cytotoxic T Cells by a Nucleoprotein/Haemagglutinin Preparation

Wraith, David C.; Askonas, Brigitte A.

doi:10.1099/0022-1317-66-6-1327

Cited by 45 publications

(34 citation statements)

References 16 publications

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“…2). HA contamination was reduced in comparison to previously reported preparations (Wraith & Askonas, 1985) by obtaining a more effective batch of bromelain.…”

Section: Np Preparationmentioning

confidence: 78%

“…A/X31 (H3N2) virus was purified and treated with bromelain as previously described (Brand & Skehel, 1972), in order to remove the majority of surface glycoproteins (> 95 ~ of HA activity). Virus cores were resuspended to 4 mg/ml and treated with 0.2~ Am.DOC (Wraith & Askonas, 1985). After 5 h the insoluble matrix (M) protein was removed by centrifugation, leaving purified NP in the supernatant (Laver & Webster, 1976).…”

confidence: 99%

“…in addition to the i.p. immunization previously described (Wraith & Askonas, 1985). A single dose of NP (I0 ~tg), administered by either route, effectively primed for (Zweerink et al, 1977 b).…”

Section: Np Priming Of Anti-influenza a Virus T C Cellsmentioning

confidence: 99%

“…SDS-PAGE of Am,DOC NP preparation. The 5 to 20~ acrylamide gradient gel was run under non-reducing conditions and influenza virus proteins were identified by mobility relative to standards as previously described (Wraith & Askonas, 1985). (a) Mol.…”

Section: Np Preparationmentioning

confidence: 99%

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Purified Influenza Virus Nucleoprotein Protects Mice from Lethal Infection

Wraith¹,

Vessey²,

Askonas³

1987

Journal of General Virology

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142

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SUMMARYLocal administration of nucleoprotein purified from X31 (H3N2) influenza A virus primed for A virus cross-reactive cytotoxic T cells and resulted in substantial protection (75 ~) of mice from a lethal challenge with the heterologous mouse-adapted A/PR/8/34 (H1N1) virus. By following the course of a lethal virus challenge we found that nucleoprotein priming did not prevent virus infection but rather aided recovery. Nucleoprotein-primed mice suffered initial symptoms of infection, i.e. weight loss and surface temperature changes, but started to recover after approximately 7 days. We suggest that such heterotypic protection can be attributed to priming of A virus crossreactive cytotoxic T cells.

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“…2). HA contamination was reduced in comparison to previously reported preparations (Wraith & Askonas, 1985) by obtaining a more effective batch of bromelain.…”

Section: Np Preparationmentioning

confidence: 78%

confidence: 99%

Section: Np Priming Of Anti-influenza a Virus T C Cellsmentioning

confidence: 99%

Section: Np Preparationmentioning

confidence: 99%

See 2 more Smart Citations

Purified Influenza Virus Nucleoprotein Protects Mice from Lethal Infection

Wraith¹,

Vessey²,

Askonas³

1987

Journal of General Virology

Self Cite

142

View full text Add to dashboard Cite

show abstract

“…It will also be crucial to find out how best these internal components can be manipulated to stimulate cellular immune responses in vivo. Both partially purified NP (Wraith & Askonas, 1985) and NP-VAC (Yewdell et al, 1986) have been shown to stimulate primary influenza A virus-specific, but virus subtype crossreactive, CTL immunity in mice in vivo.…”

Section: Discussionmentioning

confidence: 99%