Induction of ligand-specific PrP^Csignaling in human neuronal cells

Characterization of the Host Response to Pichinde Virus Infection in the Syrian Golden Hamster by Species-Specific Kinome Analysis

“…We have demonstrated that the foldchanges associated with phosphorylation events that met threshold significance criteria in our kinome analysis correlated with those found using phosphor-Western blot array analysis. The overall similarity between the kinome and Western blot results provide validity to our investigational approach and although small fluctuations in the overall fold-change were present, the specificity of phospho-specific human antibodies for hamster protein phosphorylations could contribute to fluctuations in this correlation, In addition, phosphorspecific antibody arrays provide a quantitative measure of phosphoproteins as compared with a relative measure of kinase activity, as with peptide kinome arrays and may therefore further contribute to differences in phosphorylation foldchanges between the two methodologies (49).…”

Salmonella enterica Typhimurium infection causes metabolic changes in chicken muscle involving AMPK, fatty acid and insulin/mTOR signaling

“…In addition, species-specific peptide arrays have been employed to perform this type of high-throughput analysis on species other than humans or mice [8,9]. This technique has uncovered novel biology and advanced our understanding of a number of biological processes and disease states [8-10]. In this study, we have used the species-specific peptide arrays to uncover metabolic signaling changes in chicken skeletal muscle due to Salmonella Typhimurium infection.…”

“…A more recent advancement has been the use of species-specific peptide arrays for kinomic study of less common research species such as bovine and ovine [7]. In addition, the use of these species-specific peptide arrays has branched out beyond the original host-bacterial [8] and host-viral [9] interaction studies to consider diverse biological phenomena such as prion biology [10]. As so much cellular function is controlled by protein phosphorylation, it is possible to consider other biological functions besides the standard immunity or cell growth/cycle regulation.…”

Binding of bovine T194A PrPCby PrPSc-specific antibodies

“…15 It has been demonstrated that antibodies which crosslink PrP C in neural tissue induce apoptosis 16 and our group has demonstrated that antibody binding to PrP C alters patterns of intracellular signal transduction in human neurons. 17 In contrast, the induction of an immune response which is specific for the PrP Sc conformation circumvents autoreactive PrP C antibodies and minimizes the potential adverse effects of prion immunoprophylaxis.…”