Induction of Long-Term Heart Allograft Survival in the Rat by Rabbit ATG

Mjörnstedt, L.; Olausson, Michael; Hedman, L; Lindholm, Leif; Brynger, H

doi:10.1159/000233542

Cited by 24 publications

(25 citation statements)

References 10 publications

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“…In previous experiments [7] we found that 1 ml ATG given in travenously as a single dose to WKy rats 2 days prior to transplan tation of an allogeneic PVG/c heart, resulted in over 50% long term surviving (LTS) grafts. Recipients, bearing LTS grafts for more than 200 days, were used in the present experiments.…”

Section: Induction Of Tolerancementioning

confidence: 99%

“…A largely T cell-specific rabbit-antirat ATG was produced, as described earlier [7], and the thymocytotoxic titer was adjusted to 1:1,024.…”

Section: A Tg Productionmentioning

confidence: 99%

“…Antilymphocyte serum (ALS) or globulin (ALG) has been used frequently as an immunosuppressive agent for the induction of experimental transplanta tion tolerance [1][2][3][4][5][6][7]. Several mechanisms responsible for this ALS-induced tolerance have earlier been pro posed, such as selective depletion of lymphocytes [1], blocking serum factors [5,6] or suppressor cells [2][3][4], In a previous report [7] we described a model for induction of specific transplantation tolerance in a rat heart allograft model, using antithymocyte globulin (ATG) as sole immunosuppressive agent.…”

Section: Introductionmentioning

confidence: 99%

“…Several mechanisms responsible for this ALS-induced tolerance have earlier been pro posed, such as selective depletion of lymphocytes [1], blocking serum factors [5,6] or suppressor cells [2][3][4], In a previous report [7] we described a model for induction of specific transplantation tolerance in a rat heart allograft model, using antithymocyte globulin (ATG) as sole immunosuppressive agent. In the pres ent report the mechanisms behind this tolerance were further investigated by adoptive transfer experiments, employing spleen cells or serum, either to normal or to sublethally irradiated syngeneic recipients.…”

Section: Introductionmentioning

confidence: 99%

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Suppressor Cells in Antithymocyte Globulin-Induced Transplantation Tolerance in the Adult Rat

Olausson¹,

Mjörnstedt²,

Lindholm³

et al. 1984

Int Arch Allergy Immunol

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A rat heart allograft model, for induction of transplantation tolerance by a single dose of antithymocyte globulin, was studied. Experiments were made to transfer the tolerance to syngeneic recipients, either by spleen cells or sera obtained from tolerant rats. Different doses of tolerant spleen cells caused long-term surviving (LTS) allografts when transferred to sublethally irradiated recipients. Spleen cell transfer to untreated recipients resulted in prolonged allograft survival but no LTS grafts. The results indicate the presence of a cell-mediated suppressor mechanism in the tolerant animals.

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Section: Induction Of Tolerancementioning

confidence: 99%

“…A largely T cell-specific rabbit-antirat ATG was produced, as described earlier [7], and the thymocytotoxic titer was adjusted to 1:1,024.…”

Section: A Tg Productionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Suppressor Cells in Antithymocyte Globulin-Induced Transplantation Tolerance in the Adult Rat

Olausson¹,

Mjörnstedt²,

Lindholm³

et al. 1984

Int Arch Allergy Immunol

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“…We have previously described a method for induc tion o f long-term surviving (LTS) rat heart allografts by pretreatment of the recipients with a single dose of rabbit A T G [8], The results suggested the existence of an induction phase, immediately following A T G treatment and transplantation. This period was char acterized by instability with 50% o f the allografts be ing rejected within 30 days after transplantation.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of the Induction Phase of Antithymocyte-Globulin-Induced Heart Allograft Tolerance in the Rat

Olausson

Mjörnstedt²,

Wramner³

et al. 1988

Int Arch Allergy Immunol

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Transplantation tolerance was induced in 50% of Wky rats treated with antithymocyte globulin (ATG) 2 days prior to transplantation of a PVG/c heart. The induction period immediately following ATG treatment and transplantation was characterized by instability, with 50% of the allografts being lost in rejection within 30 days after transplantation. Second syngeneic transplantation to ATG-treated recipients 5 days after the first transplantation facilitated tolerance induction and permanent acceptance of both transplants. Thirty days after ATG treatment and the primary transplantation, 50% of second syngeneic allografts were accepted. During the early period after transplantation a continuous antigen presence together with ATG was necessary. Graft removal and absence of the transplant for 3 days, close to ATG administration, resulted in rejection of a second PVG/c transplant. Furthermore, the induction phase could easily be manipulated by the addition of sensitized or tolerized spleen cells to the recipient, causing allograft rejection or tolerance, respectively. Sixty days after ATG treatment and transplantation, a longer period between graft removal and retransplantation was required to break tolerance. Injection of sensitized spleen cells at this time did not affect graft survival, indicating an increased stability of the unresponsive state 60 days after ATG treatment and transplantation.

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