Induction of macrophage DNA synthesis in vitro by non‐immunological inflammatory exudates: Effect of irradiation and thymus or bone marrow cell reconstitution

Abstract

An acute inflammatory exudate possesses mitogenic activity in that it is able to induce both DNA synthesis and proliferation of macrophages in vitro. This activity is reduced however if the inflammatory exudate is obtained from irradiated rats (900 r). Transfer of bone marrow syngeneic cells into irradiated rats does not reverse this reduction. On the contrary the decrease of mitogenic activity is more pronounced. On the other hand transfer of thymic syngeneic cells not only restores the mitogenic activity of inflammatory exudate from irradiated rats but increases it. Transfer of both types of cell together fully restores the mitogenic activity of inflammatory exudate. It is postulated that the mitogenic activity of inflammatory exudate could be related to thymic cells and that T lymphocytes may be involved in non-specific inflammatory reactions.

Induction of macrophage DNA synthesis In vitro, by non‐immunological inflammatory sera: Effect of irradiation and thymus or bone marrow cell reconstitution

“…In contrast transfer of normal thymic cells increased the mitogenic activity of exudate by 62 per cent. It was postulated that mitogenic activity in inflammatory exudate might be related to T lymphocytes (Pelletier et al, 1978).…”

The modulation of peritoneal macrophage chemiluminescence by acute pleural inflammation, prostanoids and cyclo/lipoxygenase inhibitors

“…In addition, acute inflammatory exudates stimulated, in vitro * Correspondence: Professor J.P. GIROUD, Department of Pharmacology, UA CNRS 595, CHU Cochin Port-Royal, 27, rue du Faubourg Saint-Jacques, 75674 PARIS CEDEX 14 (France). normal macrophages to divide [4] and similar activity was present in the serum of animals undergoing an acute inflammatory reaction [5,6]. These data suggest that the acute inflammatory process per se can modulate the function of macrophages in distant parts of the body.…”

Relationships between inhibition of rat polymorphonuclear chemotaxis and various inflammatory reactions

“…The presumed presence of a chemotaxis inhibitory factor within the sera of rats presenting an inflammatory reaction is paralleled by the production or release of at least one other serum factor (PELLETIER et al [5,6]) and it will be interesting to see to what extent, if any, these factors are related. The full significance of these factors has yet to be determined but it seems likely that the chemotaxis inhibitory factor represents at least part of the control mechanism regulating the.…”