Induction of multiple shoots from leaf segments, in vitro-flowering and fruiting of a dwarf tomato (Lycopersicon esculentum)

Rao, K. V.; Kasula, Kiranmayee; Umate, Pavan; Sree, T. Jaya; Rao, A. V.; Abbagani, Sadanandam

doi:10.1016/j.jplph.2005.01.008

Cited by 17 publications

(5 citation statements)

References 10 publications

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“…In our study, we observed that germinated embryo formed callus and developed lots of multiple shoots on the standardized MS medium. No hormones were required for shoot elongation and which supports that this standardized protocol is more simple than other reports in which various kinds of hormones are required for shooting, shoot elongation and rooting (Cap et al, 1991, Segeren et al, 1993b, Chen and Adachi, 1996, Dan et al, 2006, Sun et al, 2006, Rao et al, 2005, Bhattarai et al, 2009, Encina et al, 2012, Kharkongar et al, 2013). The developed interspecific hybrids showed a greater degree of morphological variations especially leaf morphology, flower types (exerted stigma), fruit’s shape, size, and colors, empty fruits due to embryo abortions, stem diameter, presence of trichomes on stem and leaves, number of seeds/fruits, number of fruits setting/plants.…”

Section: Discussionsupporting

confidence: 75%

Development of interspecific Solanum lycopersicum and screening for Tospovirus resistance

Sohrab

Bhattacharya

Rana

et al. 2015

Saudi Journal of Biological Sciences

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Tospovirus has emerged as a serious viral pathogen for several crops including tomato. The tomato production is being severely affected worldwide by Tospovirus. Some reports have been published about the association of plant virus and development of human disease either by direct or indirect consumption. Resistance to this virus has been identified as good source in wild tomato species (Lycopersicum peruvianum). But the introgression of resistance genes into cultivated tomato lines and the development of interspecific hybrid are hampered due to incompatibility, fertilization barriers and embryo abortion. But this barrier has been broken by applying the embryo rescue methods. This study describes the development of interspecific hybrid tomato plants by highly efficient embryo rescue method and screening for Tospovirus resistance. The interspecific hybrid tomato plants were developed by making a cross between wild tomato species (L. peruvianum) and cultivated tomato (Solanum lycopersicum). The immature embryos were cultured in standardized medium and interspecific hybrids were developed from embryogenic callus. The immature embryos excised from 7 to 35 days old fruits were used for embryo rescue and 31 days old embryos showed very good germination capabilities and produced the highest number of plants. Developed plants were hardened enough and shifted to green house. The hybrid nature of interspecific plants was further confirmed by comparing the morphological characters from their parents. The F1, F2 and F3 plants were found to have varying characters especially for leaf type, color of stem, fruits, size, shapes and they were further screened for virus resistance both in lab and open field followed by Enzyme linked Immunosorbant Assay confirmation. Finally, a total of 11 resistant plants were selected bearing red color fruits with desired shape and size.

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Section: Discussionsupporting

confidence: 75%

Development of interspecific Solanum lycopersicum and screening for Tospovirus resistance

Sohrab

Bhattacharya

Rana

et al. 2015

Saudi Journal of Biological Sciences

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“…In earlier studies with two species (tomato, cv. microtom, and mulberry), we have established correlations between cytokinin and auxin concentrations leading to efficient in-vitro organogenesis where the addition of IAA in combination with cytokinins (such as BAP and TDZ) to the culture media enhanced shoot elongation and supported efficient in-vitro regeneration (1,11). The present investigation established a correlation between plant growth regulator (BAP and IAA) levels and in-vitro regeneration of S. dulcis.…”

Section: Discussionsupporting

confidence: 56%

EfficientIn-VitroRegeneration from Mature Leaf Explants ofScoparia dulcisL., an Ethnomedicinal Plant

Mahender

Kokkirala

Kota

et al. 2008

Journal of Herbs, Spices & Medicinal Plants

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A plant regeneration protocol via multiple-shoot induction using leaf explants from field-grown mature plants of Scoparia dulcis L. (Scrophulariaceae), an ethnomedicinal plant, was established. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP, 13.2, 17.6 and 22.2 μM) in combination with indole-3-acetic acid (IAA, 0.5 and 2.8 μM) or napthalene-3-acetic acid (NAA, 0.5 and 2.6 μM). The maximum number of shoots (14.0 ± 1.14) with longest shoot length (2.97 ± 0.18) were obtained directly (without an intervening callus phase) from the leaf explants using a combination of BAP (22.2 μM) and IAA (0.5 μM). BAP used in combination with NAA produced fewer shoots per explant as compared with the use of IAA and the regeneration was mediated by callus formation. For root induction, the elongated shoots were separated and transferred onto MS medium supplemented with indole-3-butyric acid (IBA, 4.9 μM). Rooted Aileni et al. 201plantlets (90%) were successfully transferred to soil in the greenhouse with a 75% survival rate.

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“…1h) which occurred annually in spring even though the microenvironmental conditions were kept the same between subcultures throughout the year. In vitro flowering is not unusual (consult Chang and Chang 2003;Vaz et al 2004;McCartan and van Staden 2003;Taylor et al 2005;Venugopal Rao et al 2005;Kishor et al 2006; for a few recent examples). Few reports document in vitro flowering in the Lamiaceae; for instance rooted shoots of Basilicum polystachyon flowered after 25-30 days (Chakraborty et al 2006).…”

Section: Resultsmentioning

confidence: 99%

An efficient system for the production of clonal plantlets of the medicinally important aromatic plant: Salvia africana-lutea L.

Makunga

Staden

2007

Plant Cell Tiss Organ Cult

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Induction of multiple shoots from leaf segments, in vitro-flowering and fruiting of a dwarf tomato (Lycopersicon esculentum)

Cited by 17 publications

References 10 publications

Development of interspecific Solanum lycopersicum and screening for Tospovirus resistance

Development of interspecific Solanum lycopersicum and screening for Tospovirus resistance

EfficientIn-VitroRegeneration from Mature Leaf Explants ofScoparia dulcisL., an Ethnomedicinal Plant

An efficient system for the production of clonal plantlets of the medicinally important aromatic plant: Salvia africana-lutea L.

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