Induction of oncornavirus-like particles in cell line of canine mammary carcinoma

Abstract

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The characteristics of a canine mammary carcinoma cell line, REM 134

“…Despite these facts, there are onlv 3 reports of tissue-culture studies of canine mammary carcinomas, by Cella (1967), Owen et al (1977) and Watrach et al (1978). Owen and co-workers reported the probable establishment of 2 cell lines: one, derived from a primary adenocarcinoma, was fibroblastic in nature, while the second, from a lung metastasis, was epithelial in nature.…”

“…The cells were removed from flasks by trypsinization and processed through hypotonic KCI and fixative (Hungerford, 1965 human breast-cell lines (McGrath et al, 1974) and a canine mammary-cell line (Watrach et al, 1978). Thus to check for the presence of retroviruses in this canine mammary-cell line, labelling with [3H]-uridine was carried out at passage 120, followed by concentration of the culture supernatant and sucrose density centrifugation as outlined by Norval & Marmion (1976).…”