Induction of Ovarian Maturation and Spawning in Penaeus monodon Broodstock by Double-Stranded RNA

Treerattrakool, Supattra; Panyim, Sakol; Udomkit, Apinunt

doi:10.1007/s10126-010-9276-0

Cited by 79 publications

(49 citation statements)

References 20 publications

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“…monodon to investigate VIH (named gonad-inhibiting hormone: GIH in these reports) function, and as a means of inducing ovarian maturation and spawning on-site in Thailand, where this species is an important target of culture. The results of Treerattrakool et al (2010) show that the use of GIH-dsRNA to create GIHknockdown shrimp, is as effective as unilateral eyestalk ablation in promoting ovarian maturation and spawning. The results are very promising, and appear to be almost at the level of being able to be commercially utilized in the field.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 90%

“…Recently, Treerattrakool et al (2008Treerattrakool et al ( , 2010 have conducted studies using double-stranded RNA in Pe. monodon to investigate VIH (named gonad-inhibiting hormone: GIH in these reports) function, and as a means of inducing ovarian maturation and spawning on-site in Thailand, where this species is an important target of culture.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

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Reproductive Mechanisms in Crustacea Focusing on Selected Prawn Species: Vitellogenin Structure, Processing and Synthetic Control

Wilder¹,

Okumura²,

Tsutsui³

2010

Aqua-BioSci. Monogr. (ABSM)

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Shrimp culture is a significant world-wide industry, with current production levels reaching over 3 million tons per year. The expansion of the industry has given rise to the problems of environmental deterioration due to intensive-scale culture, and the outbreak of disease. While many of these issues are now being sufficiently addressed, the establishment of sustainable seed production technology is an area that should be given continued attention. In this regard, it remains difficult to control reproduction under hatchery conditions for a large number of commercially-important species. At present, an understanding of reproductive mechanisms in Crustacea is not complete, although in recent years, a great deal of knowledge has accumulated on vitellogenin structure, processing, and synthetic site in a number of economically-important species. This monograph will cover the current status of research on vitellogenin in decapod crustaceans, especially prawns and shrimp, and discuss mechanisms of vitellogenin synthetic control, both demonstrated and postulated. The monograph will also present current knowledge of crustacean vitellogenin receptors, and cover related facets of reproductive development, such as mechansisms of cortical rod formation and the utilization of vitellin during embryogenesis. Finally, future directions for this research and potential applications to aquaculture will be discussed.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 90%

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Reproductive Mechanisms in Crustacea Focusing on Selected Prawn Species: Vitellogenin Structure, Processing and Synthetic Control

Wilder¹,

Okumura²,

Tsutsui³

2010

Aqua-BioSci. Monogr. (ABSM)

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“…This may pave the way to develop an alternative and powerful technique in place of eyestalk ablation with similar impact. Efforts have been made to silence/knockout this gene using the technique of RNA interference by some workers but the success achieved is not as per the expectations and further research is needed in this area [25,26]. Treerattrakool et al (2011) while working on P. monodon used the technique of RNA interference for inducement of maturation both in wild and captive shrimp and reported that shrimps injected with anti-GIH double stranded (ds) RNA showed advanced maturation [25].…”

Section: Gene Editing Technologymentioning

confidence: 99%

“…Efforts have been made to silence/knockout this gene using the technique of RNA interference by some workers but the success achieved is not as per the expectations and further research is needed in this area [25,26]. Treerattrakool et al (2011) while working on P. monodon used the technique of RNA interference for inducement of maturation both in wild and captive shrimp and reported that shrimps injected with anti-GIH double stranded (ds) RNA showed advanced maturation [25]. Das et al (2015) reported silencing of gonad inhibiting hormone gene in the eyestalk neuroendocrine complex of the tiger shrimp P. monodon by using the technique of RNA interference.…”

Section: Gene Editing Technologymentioning

confidence: 99%

Gene editing (CRISPR-Cas) technology and fisheries sector

Ninawe¹,

Harke²

2017

Can J Biotech

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| P a g e AbstractConsidering the advantages of gene editing technologies, in recent years, emphasis has been given on three main techniques of gene editing i.e. zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the CRISPR/Cas9 RNA-guided endonuclease system. In all of these three technologies one thing is common i.e. the technology utilizes restriction enzymes to break down the double stranded DNA molecule at a targeted location with the help of another molecule of homologous binding protein or RNA, which is also called as a guide RNA molecule. This targeted breaking and repair of the DNA molecule as per our requirement is generally viewed as a great breakthrough in gene therapy methods. In fisheries sector, in order to promote aquaculture/mariculture activities, aquaculture industries are facing number of challenges, particularly in area of quality and demand of seed production, control of health and disease management, production of quality traits with phenotypically improved varieties, and strengthening of immune system. Several efforts are being made both at public and private sectors to develop scientific technologies to meet these challenges and substantial achievements have also been made. But still these challenges remained as major constraints in hampering the growth of this industry as per the demand and expectations. However, with the advent of now recently developed gene editing techniques, we may have to explore and evaluate the possibilities of applications of this technology as a long lasting solution in addressing at least some of the vital issues of the aquaculture industry particularly in those related to altering targeted gene structure in the species for positive impact.

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“…In recent studies, silencing of GIH expression in Penaeus monodon using GIH-specific double stranded RNA (GIH-dsRNA) increased the expression level of vitellogenin 21,22 . In a subsequent study, single injection of GIH-dsRNA into previtellogenic female P. monodon led to ovarian maturation and spawning in some prawn indicating that this method was still less effective than unilateral eyestalk ablation 23 . Dynamic levels of vitellogenin in the haemolymph were observed during reproductive activities.…”

Section: Introductionmentioning

confidence: 99%

Using vitellin monoclonal antibodies to assess the vitellogenesis-inhibiting hormone activity of Macrobrachium rosenbergii

Sripiromrak¹,

Chaivisuthangkura²,

Longyant³

et al. 2014

ScienceAsia

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Vitellogenesis in crustaceans is under the inhibitory regulation of the endocrine system in the X-organ/sinus gland complex in the eyestalks. In this study, two monoclonal antibodies specific to Macrobrachium rosenbergii vitellin and vitellogenin were generated to assess vitellogenesis-inhibiting hormone (VIH) activity. A combination of two monoclonal antibodies specific to the 89 and 100 kDa subunits of vitellin were used to develop a competitive enzyme-linked immunosorbent assay protocol for the quantitative determination of vitellogenin levels in haemolymph. Injection of eyestalk extracts from M. rosenbergii dissolved in either saline or in methanol-acetic acid caused a reduction in vitellogenin levels in the haemolymph at 12 and 24 h post-injection in bilateral eye-ablated prawns with developing ovaries. In contrast, the application of the heterologous eyestalk extract from Penaeus vannamei did not reduce vitellogenin levels in haemolymph. This assay is therefore specific and could be used to assess VIH activity during hormone purification.

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Induction of Ovarian Maturation and Spawning in Penaeus monodon Broodstock by Double-Stranded RNA

Cited by 79 publications

References 20 publications

Reproductive Mechanisms in Crustacea Focusing on Selected Prawn Species: Vitellogenin Structure, Processing and Synthetic Control

Reproductive Mechanisms in Crustacea Focusing on Selected Prawn Species: Vitellogenin Structure, Processing and Synthetic Control

Gene editing (CRISPR-Cas) technology and fisheries sector

Using vitellin monoclonal antibodies to assess the vitellogenesis-inhibiting hormone activity of Macrobrachium rosenbergii

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