Induction of ovulation with buserelin in jennies: In search of the minimum effective dose

Camillo, Francesco; Vannozzi, Iacopo; Tesi, Matteo; Sabatini, C; Rota, Alessandra; Paciolla, E; Dang-Nguyen, Irene; Panzani, Duccio

doi:10.1016/j.anireprosci.2014.09.011

Cited by 23 publications

(33 citation statements)

References 20 publications

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“…The second and third experiments show that lower doses of buserelin (3, 2 and 1 mg) have the same efficacy when compared with the 6 mg buserelin and hCG treatments. These results are in agreement with those obtained in studies conducted after or at the same time as those presented here (Newcombe & Cuervo‐Arango, in mares and Camillo et al, in jennies). In a previous study, presented at the international symposium, when different teams tested deslorelin in long‐acting formulations, Lindholm, Bloemen, Brooks, Ferris, and Mc Cue () tested buserelin and deslorelin acetate in saline solution on low number of mares.…”

Section: Discussionsupporting

confidence: 93%

“…To inject large doses of buserelin, Levy and Duchamp () used buserelin approved for human treatment (Suprefact ® ) which is available lyophilized (6 mg/bottle) to dilute in saline solution to 1 mg/ml. Since this first paper, but after that our studies have been conducted, few other studies about large doses of buserelin acetate in saline solution as trigger for ovulation in mares (Newcombe & Cuervo‐Arango, ) and in jennies (Camillo et al, ) were published. In France, production and marketing of Suprefact ® were stopped in 2016, since the high doses of buserelin are no longer available.…”

Section: Introductionmentioning

confidence: 99%

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Single injection of triptorelin or buserelin acetate in saline solution induces ovulation in mares the same as a single injection of hCG

Dordas-Perpinyà

Normandin²,

Dhier³

et al. 2020

Reprod Domestic Animals

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The aim of this study was to assess the efficacy of different doses of buserelin acetate and another GnRH agonist, triptorelin acetate, in saline solution in a single subcutaneous injection, to induce ovulation of growing pre‐ovulatory follicle in mare and compare it with the classical treatment of a single injection of hCG. The study is split into 3 experiments over different breeding seasons in the same stud with a random distribution of treatment. The first one was to compare the injection of 6 mg of buserelin with 1,500 IU of hCG; the second one consisted of comparing different doses of buserelin (6 mg and 3 mg); and the third one compared three different doses of buserelin (3, 2 and 1 mg), 0.1 mg of triptorelin with 1,500 IU of hCG as a control group. The results of all experiments showed the same efficacy between all treatments with mares ovulating between 24 and 48 hr after injection: experiment 1: hCG (78% n = 41) and buserelin 6 mg (90% n = 50); experiment 2: buserelin 6 mg (78,1% n = 192) and buserelin 3 mg (78% n = 341); and experiment 3: hCG (87% n = 106), buserelin 3 mg (84,7% n = 137), buserelin 2 mg (82,7% n = 104), buserelin 1 mg (87% n = 54) and triptorelin 0.1 mg (84,7% n = 72). In conclusion, this study contributes to erasing the dogma that has been established since 1975 that a single injection in solution without any long‐acting excipient of a GnRH agonist cannot induce ovulation in the mare. This study also shows that a injection of 0.1 mg of triptorelin in solution is a good alternative for ovulation induction and is comparable to small doses of buserelin acetate in solution (1 mg) and 1,500 IU of the gold standard trigger hCG, mainly in countries where human formulation of buserelin is not available.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Single injection of triptorelin or buserelin acetate in saline solution induces ovulation in mares the same as a single injection of hCG

Dordas-Perpinyà

Normandin²,

Dhier³

et al. 2020

Reprod Domestic Animals

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“…In Brazil, Deslorelin is currently being used for induction of single, or more particularly, multiple ovulations [3,4,25,35,36,38]. As described here, Buserelin was considered effective since induced ovulation frequency did not differ from the obtained with hCG, and were also not different from those reported by authors using this agent [6,9,26] or other synthetic analogs [2,10].…”

Section: Discussionmentioning

confidence: 81%

Induction of Ovulation in Mangalarga Marchador Mares by hCG or GnRH

Ferreira-Silva¹,

Nascimento²,

Moura³

et al. 2018

Acta Scientiae. Vet.

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Background: Induction of ovulation is a key procedure for horse assisted reproduction technologies, such as for artificial insemination (AI) and embryo transfer. The application of hCG remains as the primary ovulation-inducing agent for horse assisted reproduction, but alternatives are in demand to avoid its adverse effects, such as loss of ovulation-inducing efficiency over multiple applications by hCC-antibody production. Despite reports on alternative ovulation-inducing agents, pair-wise comparisons of such agents under similar conditions have been limited. Under such scenario, the work was aimed to determine the efficiency of both hCG and Buserelin at inducing ovulation in Mangalarga Marchador mares raised in the Northeast of Brazil under an AI program.Materials, Methods & Results: Mares were initially selected based on their reproductive performance, the absence of clinical-reproductive alterations and adequate body condition score. Mares in diestrus were randomly distributed in three experimental conditions, received 5 mg of Dinoprost and were monitored daily for estrus detection. After estrus detection, ovaries were monitored by ultrasonography, in 12-h intervals, until the follicle reached 35 mm. At this time-point, ovulation was induced with 0.042 mg of Buserelin endovenously, with 3,000 IU hCG by an intramuscular shot, and control mares received 2 mL of saline solution, also by an intramuscular shot. Both hCG and Buserelin displayed similar efficiencies (P > 0.05) for induction of ovulation and that both agents were effective (P < 0.05) for such purpose, since greater percentages (P < 0.05) of induction on mares treated from those of the control. Moreover, the total number of ovulations in mares treated at the end of the experiment was not different (P > 0.05) from those found in the Control. All ovulations occurred within a 72-h period after treatment. It can be observed that in mares treated with hCG or Buserelin, ovulations occurred both in more mares (P < 0.05) and at earlier time-points than mares from the control. It is also possible to note that pregnancy was not different (P > 0.05) between hCG and Buserelin groups, and that pregnancy of mares treated with ovulation-inducing factors was similar to the control.Discussion: The majority of ovulations in mares occurred within initial 48-h after treatment for both hCG and GnRH, suggesting a similar potential for horse assisted reproduction. Both hCG and Buserelin are two commonly used agents for induction of ovulation in mares. As described here, the majority of ovulations occurred within initial 48-h after treatment, a fact which can be attributed to hCG and GnRH activity, since it can happen in intervals from 36 to 48-h after treatment. Pregnancy rates did not differ among groups. These results are under the working hypothesis that hCG and Buserelin would display similar efficiencies on pregnancy rates. Despite the understanding of hCG activity on induction of ovulation due to its high specificity toward LH receptors and results from a direct effect on diminishing estradiol concentration, increasing LH, and further inducing ovulation within 48-h after treatment. In contrast, Buserelin has a similar functional property but acts upon LH synthesis and its release. In conclusion, ovulation in mares can be induced with both hCG and Buserelin, and both ovulation-inducing agents do not affect pregnancy rates.Keywords: gonadotropin, releasing factor, ovary, follicle, corpus luteum.

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“…The hormonal agents commonly used for the induction of ovulation in mares are human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone (GnRH). The efficacy of hCG and GnRH for inducing ovulation in jennies has been reported by a few authors (Carluccio et al 2007;Camillo et al 2014). Human chorionic gonadotropin is a hormone the biological activity of which is similar to the luteinizing hormone (LH).…”

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confidence: 99%

“…Carluccio et al (2007) reported that even if hCG administration is the most efficient method for prediction and synchronization of ovulation, current results indicate that, unlike in the mare, even a single administration of lecirelin (GnRH-analogue) can successfully hasten ovulation in jennies where a follicle equal to larger than 30 mm has been detected. The study from Camillo et al (2014) showed the possibility of inducing ovulation in jennies between 24 and 48 h with a single subcutaneous injection of a very low dose of GnRH agonist buserelin. The effect of a single administration of GnRH analogue lecirelin in the jenny differs from that reported in the mare (Carluccio et al 2007).…”

mentioning

confidence: 99%

The effect of hCG, GnRH and PGF2α analogue cloprostenol on the oestrus cycle in jennies

et al. 2019

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The objectives of this study were twofold. Firstly, the present study was designed to examine susceptibility of the corpus luteum (CL) in early diestrus in jennies; and secondly, to investigate the effect of two commonly used hormonal agents in horses on the induction of ovulation in jennies. The oestrus cycles of eleven jennies were monitored by ultrasound every day. When the dominant follicle reached a diameter of 30 mm, the jennies were treated by intramuscular administration of gonadotropin-releasing hormone agonist lecirelin (GnRH, 50 µg pro toto) in the first oestrus cycle, and human chorionic gonadotropin (hCG, 1500 IU pro toto) intramuscularly in the second oestrus cycle. Prostaglandin F2α analogue cloprostenolum (PGF2α, 0.125 mg pro toto) was administered intramuscularly 2 days after the first ovulation and the interovulatory interval was monitored. This study showed that intramuscular administration of 50 µg of GnRH agonist lecirelin resulted in ovulation within 48 h in 73% of treated jennies. Intramuscular administration 1500 IU of hCG was found to be poorly effective to induce ovulation, with 36% of animals ovulating within 48 h. Intramuscular administration of PGF2α analogue cloprostenol 2 days after ovulation was unsuccessful in attempting to shorten the interovulatory interval in donkeys.

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Induction of ovulation with buserelin in jennies: In search of the minimum effective dose

Cited by 23 publications

References 20 publications

Single injection of triptorelin or buserelin acetate in saline solution induces ovulation in mares the same as a single injection of hCG

Single injection of triptorelin or buserelin acetate in saline solution induces ovulation in mares the same as a single injection of hCG

Induction of Ovulation in Mangalarga Marchador Mares by hCG or GnRH

The effect of hCG, GnRH and PGF2α analogue cloprostenol on the oestrus cycle in jennies

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