Induction of oxidative stress and antioxidant defenses in the livers of sea trout, Salmo trutta L., with ulcerative dermal necrosis

Kurhalyuk, N.; Tkachenko, Halyna

doi:10.2478/v10086-011-0029-0

Cited by 9 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, Providencia rettgeri infection induces oxidative damage in infected O. niloticus via increasing hepatic and renal MDA levels and reducing SOD and catalase activities [116]. Similarly, the SOD and GSH levels were diminished with higher ROS levels but were not affected in the liver and kidney of silver catfish (Rhamdia quelen) experimentally infected with Aeromonas caviae, and in the liver of sea trout (Salmo trutta L.) experimentally infected with A. hydrophila [114,117]. Our data demonstrated that Vibrio and Enterococcous infection caused the generation of ROS (higher MDA levels) with an impairment of antioxidant molecules (reduction of GSH, SOD, and catalase) in both the gills and brain of the diseased flathead grey mullet compared to the control one.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Co-Infection as a Potential Threat to Farmed Flathead Grey Mullet (Mugil cephalus): Phenotypic and Molecular Diagnosis, Histopathology, Immunity Response, and In Vitro Antibacterial Evaluation

Elbahnaswy

Elshopakey

Shakweer

et al. 2023

Fishes

View full text Add to dashboard Cite

Vibrio alginolyticus, Vibrio chlorea, and Enterococcus cloacae were the main bacterial pathogens investigated during recurrent episodes of mortalities among farmed flathead grey mullet (Mugil cephalus) in the Bahar El-Baqar area, Sharqia governorate, Egypt, during the summer season in 2021. Nevertheless, the contamination of water with heavy metals was evaluated. In this study, a total number of sixty moribund and freshly dead Mugil cephalus were examined clinically and upon post-mortem. Then, the bacterial isolates were subjected to bacteriological phenotypic examination, 16S rRNA gene sequencing, and antibiotic sensitivity testing. Histopathological screening and the immunity response of naturally infected M. cephalus were also implemented. In areas where bacterial strains were recovered in a high percentage, water contamination with Cu, Fe, and Ni was higher than the permissible limit. The results showed a high similarity (99–100%) to Vibrio and E. cloacae strains using phylogenetic analysis of 16S rRNA. Most isolates exhibited sensitivity to florfenicol, ciprofloxacin, and norfloxacin, while they were resistant to erythromycin and amoxicillin. Hemorrhage, oedema, hemosiderosis, and melanomacrophage activation in most affected tissues of infected M. cephalus were detected by light microscopy. Nevertheless, higher levels of AST and ALT, as well as serum inflammatory markers, CRP, and lysozyme activity, were shown in infected fish. There were higher MDA levels, along with a reduction of GSH, SOD, and catalase, in the gills and brain of infected M. cephalus, compared to the control fish. Our obtained data confirmed the high prevalence of Vibrio serovars and E. cloacae in infected grey mullets, describing their histopathological alterations, higher inflammatory response, and oxidative stress impact. These results could be useful indicators for understanding the pathogenesis of these pathogens in correlation with water parameter deterioration. Thus, the emergence of Vibrio and E. cloacae species in this study threatens the brackish water aquaculture industry in Egypt and poses a public health concern.

show abstract

Section: Discussionmentioning

confidence: 99%

Bacterial Co-Infection as a Potential Threat to Farmed Flathead Grey Mullet (Mugil cephalus): Phenotypic and Molecular Diagnosis, Histopathology, Immunity Response, and In Vitro Antibacterial Evaluation

Elbahnaswy

Elshopakey

Shakweer

et al. 2023

Fishes

View full text Add to dashboard Cite

show abstract

“…Для оценки негативного влияния паразитарных инвазий на состояние здоровья рыб рекомендовано использовать показатели ПОЛ и ОМБ, отражающие уровень тканевого повреждения при окислительном стрессе. Так, увеличение содержания продуктов ПОЛ и ОМБ было показано в печени кумжи Salmo trutta при язвенном некрозе кожи, вызванном бактериями Aeromonas hydrophila (Kurhalyuk & Tkachenko, 2011). В исследованиях на условно здоровом (10 или меньше экз.)…”

Section: Discussionunclassified

Pathomorphological and biochemical study of the golden grey mullet Chelon auratus (Risso, 1810) in the waters of the southwestern Crimea (the Black Sea)

Gavruseva

Sigacheva

Чеснокова

2022

mbj

View full text Add to dashboard Cite

The golden grey mullet Chelon auratus (Risso, 1810) (Mugilidae) is a valuable commercial and recreational species ranking first in terms of catch volume of the Black Sea indigenous mullets. The importance of this species in the regional fishery among demersal fish requires the development of a system for assessing its health status. Such research is based on an integrated approach involving biochemical and pathomorphological methods: these allow to investigate the alterations in fish prior the occurrence of visible manifestations, disruption of the processes of growth and reproduction, reduction of commercial size, and decrease in abundance. The aim of our work was to study both pathomorphological alterations and several biochemical parameters of golden grey mullet tissues for assessing its health status. Fish visual examination and pathological autopsy were carried out. For histological analysis, samples of the gills, liver, kidneys, gastrointestinal tract, spleen, and pancreas were fixed in Davidson’s solution and processed by standard methods. Based on the histological studies, the fish health status was investigated by a modified semi-quantitative analysis of alterations according to the Bernet et al. protocol and by assessing the distribution of lesion in organs using a scoring system. We determined the importance factors of alterations for C. auratus, the values of organ alteration indices, and the total index of fish pathology. The biochemical studies permitted to reveal the level of protein oxidation, lipid and urea peroxidation, and the activity of aminotransferases and alkaline phosphatase in the liver; moreover, we quantified albumin and glucose concentration in the blood serum. In the organs of the golden grey mullet, the histopathological alterations referring to four types of the reaction patterns were detected (circulatory disorders, regressive and progressive alterations, and inflammatory processes). Furthermore, parasites representing several species of different systematic groups (Protozoa, Monogenea, Trematoda, and Nematoda) were identified. It was established that the most severe histopathological alterations were caused by a parasitic protozoan, presumably Ichthyophonus sp. When carrying out a semi-quantitative analysis of alterations, the mullets were conventionally divided into conditionally healthy individuals and infected ones. Pathomorphological data were obtained, and the set of biochemical parameters was compared in these two groups. Significant differences were revealed in the values of organ alteration indices in C. auratus in the kidneys, liver, gastrointestinal tract, and pancreas. The values of the total index of fish pathology also differed significantly. The biochemical studies revealed a significant increase in urea content in the liver of fish from the group 2, that may indicate the kidney and gill excretory dysfunction (it was confirmed histologically). No significant differences were found in the level of lipid peroxidation, protein oxidation, and activity of aminotransferases in the liver of conditionally healthy and infected fish. The results of our investigation confirm high informativeness of the studied parameters for assessing the health status of the golden grey mullet.

show abstract

“…Antioxidant enzymes are good biomarkers for oxidative stress; they play a crucial role in competing the damaging effects of oxidative stress that results from over production of ROS (Kurhalyuk & Tkachenko, ). Where they are key components that protect cells, and attempts to save species’ survivability during oxidative stress (Lushchak & Bagnyukova, ).…”

mentioning

confidence: 99%

The extent to which lipopolysaccharide modulates oxidative stress response inMugil cephalusjuveniles

et al. 2019

View full text Add to dashboard Cite

Induction of oxidative stress and antioxidant defenses in the livers of sea trout, Salmo trutta L., with ulcerative dermal necrosis

Cited by 9 publications

References 28 publications

Bacterial Co-Infection as a Potential Threat to Farmed Flathead Grey Mullet (Mugil cephalus): Phenotypic and Molecular Diagnosis, Histopathology, Immunity Response, and In Vitro Antibacterial Evaluation

Bacterial Co-Infection as a Potential Threat to Farmed Flathead Grey Mullet (Mugil cephalus): Phenotypic and Molecular Diagnosis, Histopathology, Immunity Response, and In Vitro Antibacterial Evaluation

Pathomorphological and biochemical study of the golden grey mullet Chelon auratus (Risso, 1810) in the waters of the southwestern Crimea (the Black Sea)

The extent to which lipopolysaccharide modulates oxidative stress response inMugil cephalusjuveniles

Contact Info

Product

Resources

About