The extent to which lipopolysaccharide modulates oxidative stress response in<i>Mugil cephalus</i>juveniles

Abdel‐Mageid, Afaf D.; Zaki, Abeer Gamal; Senosi, Yakout A. El; Asely, Amel M. El; Fahmy, Hanan A.; El‐Kassas, Seham; Abo‐Al‐Ela, Haitham G.

doi:10.1111/are.14309

Cited by 12 publications

(4 citation statements)

References 44 publications

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“…In a previous study, investigators confirmed a significant modulation of the antioxidative response of grey mullet following LPS injection with different doses and exposure times (Abdel‐Mageid et al, ). In this study, we hypothesized that exposure to different stressors at the early stage of life differentially modulates the immune status of M. cephalus depending on dose and exposure time of these stress factors.…”

Section: Introductionmentioning

confidence: 67%

“…LPS is a well‐known endotoxin that can effectively evoke the immune system. LPS induces cytokine and antibody production and modulates the oxidative stress response (Abdel‐Mageid et al, ; Al‐Harbi & Austin, ; Byadgi et al, ; Kadowaki et al, ; Selvaraj, Sampath, & Sekar, ). A large number of immune‐related cDNAs were retrieved after LPS exposure in rainbow trout.…”

Section: Discussionmentioning

confidence: 99%

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Modulatory effect of lipopolysaccharide on immune‐related gene expression and serum protein fractionation in grey mullet,Mugil cephalus

et al. 2020

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Immunity occupies the top priority in aquaculture. Great efforts have been made to study fish immunity, but little is known about the immunity of grey mullet, Mugil cephalus despite of its importance. We evaluated the effect of different doses (0, 1, 10 and 100 µg/kg body weight) of lipopolysaccharide (LPS) on the expression of non‐specific immune response genes (interleukin (il) 1β, il8, il10 and tumour necrosis factor alpha (tnfα)) in the liver, as well as its effect on the fractionation of serum protein in juvenile grey mullet along different times and concentrations. Analysis revealed that LPS initially caused an upregulation in the studied cytokines. After that, transcripts levels were switched sequentially between downregulation and upregulation, ending with an upregulation at 1 week post injection in il8, il10 and tnfα. Regarding il1β, its initial upregulation was followed by gradual decreases, which reached a marked downregulation at 1 week post injection. Protein fractionation exhibited many changes among doses and time. Nine fractions were discernable in protein electrophoresis of serum in all doses. The most pronounced changes were detected in fractions 7–9 (generally refer to γ globulins or immunoglobulins) using densitometric analysis. In addition, LPS suggested to modulate the levels of serum transferrin. It is known that LPS is a component of the cell wall of Gram negative bacteria, and this study sheds light on the molecular fitness mechanisms against bacterial infection, which could help in developing a strategy to improve resistance to bacterial diseases, or possibly to enhance immune response in grey mullet.

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Section: Introductionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Modulatory effect of lipopolysaccharide on immune‐related gene expression and serum protein fractionation in grey mullet,Mugil cephalus

et al. 2020

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“…The immune system plays an essential and effective role in the regeneration process. There is a strong link between antioxidant defense machinery and immune systems (Abdel‐Mageid, Zaki, El Senosi, El Asely et al, 2020; Abdel‐Mageid, Zaki, El Senosi, Fahmy et al, 2020; Abo‐Al‐Ela, 2018a, 2018b, 2019a). Signaling of reactive oxygen species (ROS) after axolotl tail amputation is required for early phases of regeneration.…”

Section: Potential Contributions Of the Immune System In Regenerationmentioning

confidence: 99%

Exploring the role of microRNAs in axolotl regeneration

Abo‐Al‐Ela

Burgos-Aceves

2020

Journal Cellular Physiology

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The axolotl, Ambystoma mexicanum, is used extensively for research in developmental biology, particularly for its ability to regenerate and restore lost organs, including in the nervous system, to full functionality. Regeneration in mammals typically depends on the healing process and scar formation with limited replacement of lost tissue. Other organisms, such as spiny mice (Acomys cahirinus), salamanders, and zebrafish, are able to regenerate some damaged body components. Blastema is a tissue that is formed after tissue injury in such organisms and is composed of progenitor cells or dedifferentiated cells that differentiate into various cell types during regeneration. Thus, identifying the molecules responsible for initiation of blastema formation is an important aspect for understanding regeneration. Introns, a major source of noncoding RNAs (ncRNAs), have characteristic sizes in the axolotl, particularly in genes associated with development. These ncRNAs, particularly micro-RNAs (miRNAs), exhibit dynamic regulation during regeneration. These miRNAs play an essential role in timing and control of gene expression to order and organize processes necessary for blastema creation. Master keys or molecules that underlie the remarkable regenerative abilities of the axolotl remain to be fully explored and exploited. Further and ongoing research on regeneration promises new knowledge that may allow improved repair and renewal of human tissues.

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“…The expression of several immune‐related genes, including IL‐1β, IL‐10 and TNF‐α, is efficiently up‐regulated by lipopolysaccharides (Hardie et al, 1994). In a prior study, researchers reported that grey mullet antioxidation response was significantly modulated by LPS injection at various doses and at different times (Abdel‐Mageid, Zaki, El Senosi, El Asely, et al, 2020). LPS induces the development and regulation of oxidative stress response by cytokines and antibodies (Abdel‐Mageid, Zaki, El Senosi, El Asely, et al, 2020), which supported that LPS produces high total antibody headings titre as recorded for many species of fish (Hang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Ameliorative effect of dietary lipopolysaccharides on Oreochromis niloticus juveniles submitted to aflatoxin B1‐induced oxidative stress and bacterial challenge

El‐Kader¹,

Shukry

Dawood³

et al. 2021

Aquaculture Research

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The current study was designed to evaluate the ameliorative effect of lipopolysaccharides (LPS) (a portion of the Gram -ve bacteria cell wall) on aflatoxin (AFB1)-persuaded changes in the cytokine expression, heat-shock protein 70 (HSP70), antioxidant genes, different haematological and biochemical parameters in Nile Tilapia along with the infection by Aeromonas hydrophila and the immune-stimulant efficiency of LPS on the growth pattern. For this study, two hundred and forty O. niloticus (35 ± 1.5 g) were used. The fish were randomly allocated into four experimental groups, four replicates for each group (15 fish/replicate). Group 1 was the negative control group fed on a basal diet, and group 2 was the positive control group fed on the basal diet with aflatoxins (AFB1) 200 µg/kg feed. Group 3 has fed the lipopolysaccharides 20 mg/100 gm diets. Group 4, besides the AFB1-contaminated basal diet, was provided with lipopolysaccharides 20 mg/100 gm diet. At the end of the 12 weeks, each group was injected with A. hydrophila. The analysis revealed that LPS-treated AFB1supplemented fish showed improvement in haematological, lysosomal and phagocytic activities and total protein and significantly decreased liver enzyme exaggerated by aflatoxin; alongside, the LPS-treated aflatoxin-supplemented fish revealed a significant effect on the haematological and the immunity markers after 24 h, 48 h and 1 week post challenged with A. hydrophila. Concerning the gene expression, AFB1 group showed significant up-regulation of HSP70 and interferon gamma gene mRNA expression, which is significantly down-regulated by LPS treatment, besides LPS showed up-regulation of catalase and superoxide dismutase genes markedly alongside with interleukins (IL), IL-8 and IL-1B; the binding effect of LPS markedly appears after A. hydrophila which significantly modulates the HSP70 and immunity marker genes in addition to the antioxidant-related genes, CAT and SOD. Histopathological findings reflected a marked increase in intestinal villi length in LPS-treated groups.Our view illuminates for the first time the pathways of molecular wellness for the role of LPS in AFB1 toxicity and bacterial infection. Our observation illuminates the | 3661 ABD EL-KADER Et AL.

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The extent to which lipopolysaccharide modulates oxidative stress response inMugil cephalusjuveniles

Cited by 12 publications

References 44 publications

Modulatory effect of lipopolysaccharide on immune‐related gene expression and serum protein fractionation in grey mullet,Mugil cephalus

Modulatory effect of lipopolysaccharide on immune‐related gene expression and serum protein fractionation in grey mullet,Mugil cephalus

Exploring the role of microRNAs in axolotl regeneration

Ameliorative effect of dietary lipopolysaccharides on Oreochromis niloticus juveniles submitted to aflatoxin B1‐induced oxidative stress and bacterial challenge

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