Induction of pathogenesis-related proteins in germinating wheat seeds infected with Fusarium culmorum

Caruso, Carla; Chilosi, G.; Caporale, Carlo; Leonardi, Luca; Bertini, Laura; Magro, P.; Buonocore, Vincenzo

doi:10.1016/s0168-9452(98)00199-x

Cited by 107 publications

(67 citation statements)

References 56 publications

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“…The water potentials of the 14-3-3λ mutants were significantly increased during drought and their relative water contents were also significantly reduced compared to the wild-type. Cell membrane disruption caused by drought is typically assessed by measuring ion leakage (Caruso et al, 1999). The conductance of ions was increased significantly in 14-3-3 mutants compared to wild-type in drought conditions.…”

Section: Discussionmentioning

confidence: 99%

Physiological and Mechanical Role of 14-3-3 Lambda in Arabidopsis thaliana during Drought Stress

Peethambaran¹,

Li²,

Dzugan³

et al. 2012

JAS

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Climate changes this century due to global warming compel a greater understanding of molecular mechanisms for plant resistance to drought conditions. Plants undergo changes in their anatomy, morphology, mechanical properties and also in their gene expression in response to environmental stress. Using reverse genetics, anatomy and molecular biology approaches, the role of a 14-3-3 protein isoform in the presence of simulated drought conditions in the model organism Arabidopsis thaliana was investigated. The14-3-3 proteins serve the function of signal transduction in eukaryotes and their role in several abiotic and biotic stress conditions have been previously reported. In this study we used 12 isoforms of 14-3-3 that are expressed and well characterized in A. thaliana to determine which isoform of 14-3-3 contributed to drought stress resistance. A phenotypic assay for drought was conducted by growing all the mutants of the isoforms in media with Abscisic acid (ABA). The 14-3-3 lambda (λ) mutants showed deficiency in lateral root growth. Further investigations revealed significant differences in ion leakage, absolute water content, water potential and tensile strength in mutants deficient in 14-3-3λ versus wild-type (Columbia-0). Leaf anatomy of the 14-3-3λ mutants demonstrated a greater cell density and less intracellular airspace compared to Col-0 in well-hydrated and drought conditions. Roots of the 14-3-3λ mutants exhibited collapsed epidermal, endodermal and cortical cells in both wet and dry conditions. We thus conclude that 14-3-3λ is involved in drought tolerance and contributes in the development of roots and leaves crucial for drought resistance.

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Section: Discussionmentioning

confidence: 99%

Physiological and Mechanical Role of 14-3-3 Lambda in Arabidopsis thaliana during Drought Stress

Peethambaran¹,

Li²,

Dzugan³

et al. 2012

JAS

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“…The barley seed peroxidase isozymes could be involved in defense against different pathogens. Infection by F. culmorum caused an increase in peroxidase activity from the early stages of development of inoculated wheat seedlings 47 . An increase in BP1 in black pointed barley grain, undesirable to the malting industry, has been observed using proteomics 206 .…”

Section: Peroxidases (Pr-9)mentioning

confidence: 97%

“…Amplification of PRs effectiveness through synergism enables achievement of a wide range of target specificities by combinatorial interactions. Coordinated induction of different PRs may create considerably broader resistance 47,353 .…”

Section: Biological Function and Mechanism Of Actionmentioning

confidence: 99%

“…F. culmorum infection of wheat results in the induction of several glucanase and chitinase isoforms, while some isoforms of both families accumulate constitutively 47 . The hypothesis that the seed hydrolytic enzymes, glucanase and chitinase, are part of a plant strategy to protect a germinating seed against microbial attack was supported by the finding that several barley seed isoforms of both proteins, mostly basic ones, act in vitro against seed patho- gens 181 .…”

Section: Glucanases (Pr-2)mentioning

confidence: 99%

“…The conserved region, common to both classes is the barwin domain, named after the first characterized member of the PR-4 family, barley wound induced protein. The PR-4 genes are either developmentally regulated in cereal seeds, or induced by F. culmorum infection 47 , by Clonostachys rosea, a common worldwide saprophyte with a destructive effect against several plant pathogenic fungi 280 and by elevated temperatures 3 .…”

Section: Barwin (Barley Wound Induced Protein) (Pr-4)mentioning

confidence: 99%

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A Review: Biological and Technological Functions of Barley Seed Pathogenesis-Related Proteins (PRs)

Gorjanović

2009

Journal of the Institute of Brewing

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The mature barley seed proteome is dominated by proteins involved in stress responses, including the Pathogenesis-Related proteins (PRs). PRs are currently classified into 17 families. The biochemistry of the PRs families, whose members are constitutively present in barley seed, is surveyed in this paper. These proteins are assumed to protect dormant and germinating seeds against pathogenic microorganisms and pests. The current knowledge on PRs structural and functional properties is summarized in an attempt to illustrate their importance in barley seed innate resistance. At the same time, a platform to understand PRs technological function in cereal foods and in beverage processing and quality is provided.

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Contribution of cell wall degrading enzymes to pathogenesis of Fusarium graminearum: a review

Kikot

Hours

Alconada

2009

J. Basic Microbiol.

207

140

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Plant-pathogenic fungi produce an array of extracellular hydrolytic enzymes that enable them to penetrate and infect the host tissue; these enzymes are collectively called cell wall-degrading enzymes (CWDE). They may contribute to pathogenesis by degrading wax, cuticle and cell walls, thus aiding tissue invasion and pathogen dissemination. Furthermore, they can act as elicitors of host defense reaction.Fusarium head blight (FHB) is a disease caused principally by Fusarium graminearum on crops, occurring all over the world. Important economic losses on wheat-growing areas have been registered by altering quality parameters of grains. Significant progress has been made in understanding the infection process from F. graminearum on wheat, based on genomic technologies. The virulence degree of this phytopathogen on crops could arise from differences in the production of extracellular enzymes, factors controlling the establishment of infection.Fusarium graminearum isolates from different geographical areas have been examined, and a combination of morphological and molecular data allowed the division of fungi in diverse groups, which have been related to the variation in pathogenicity. In most studied cases there is a correlation between the presence of pectic enzymes, disease symptom and virulence, being also their production decisive in the infection process.

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Induction of pathogenesis-related proteins in germinating wheat seeds infected with Fusarium culmorum

Cited by 107 publications

References 56 publications

Physiological and Mechanical Role of 14-3-3 Lambda in Arabidopsis thaliana during Drought Stress

Physiological and Mechanical Role of 14-3-3 Lambda in Arabidopsis thaliana during Drought Stress

A Review: Biological and Technological Functions of Barley Seed Pathogenesis-Related Proteins (PRs)

Contribution of cell wall degrading enzymes to pathogenesis of Fusarium graminearum: a review

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