Induction of programmed cell death in <i>Arabidopsis</i> and rice by single‐wall carbon nanotubes

Shen, Cong-Xiang; Zhang, Quanfang; Li, Jian; Bi, Fangcheng; Yao, Nan

doi:10.3732/ajb.1000073

Cited by 235 publications

(122 citation statements)

References 42 publications

(62 reference statements)

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“…Arabidopsis thaliana wild-type (Columbia) and acd5 mutant plants (Greenberg et al, 2000) were grown on soil under fluorescent lights as described by Shen et al (2010). The double mutants acd5 loh2 and acd5 loh3 were generated by crossing acd5 with loh2 (SALK_018608) or loh3 (SALK_150849), respectively, and isolated from the F2 progeny by PCR genotyping for T-DNA insertion in loh2 or loh3 and sequencing for the point mutation in acd5 (Supplemental Table 1).…”

Section: Plants and Materialsmentioning

confidence: 99%

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Loss of Ceramide Kinase in Arabidopsis Impairs Defenses and Promotes Ceramide Accumulation and Mitochondrial H₂O₂ Bursts

Liu

et al. 2014

Plant Cell

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146

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Arabidopsis thaliana plants that lack ceramide kinase, encoded by ACCELERATED CELL DEATH5 (ACD5), display spontaneous programmed cell death late in development and accumulate substrates of ACD5. Here, we compared ceramide accumulation kinetics, defense responses, ultrastructural features, and sites of reactive oxygen species (ROS) production in wild-type and acd5 plants during development and/or Botrytis cinerea infection. Quantitative sphingolipid profiling indicated that ceramide accumulation in acd5 paralleled the appearance of spontaneous cell death, and it was accompanied by autophagy and mitochondrial ROS accumulation. Plants lacking ACD5 differed significantly from the wild type in their responses to B. cinerea, showing earlier and higher increases in ceramides, greater disease, smaller cell wall appositions (papillae), reduced callose deposition and apoplastic ROS, and increased mitochondrial ROS. Together, these data show that ceramide kinase greatly affects sphingolipid metabolism and the site of ROS accumulation during development and infection, which likely explains the developmental and infection-related cell death phenotypes. The acd5 plants also showed an early defect in restricting B. cinerea germination and growth, which occurred prior to the onset of cell death. This early defect in B. cinerea restriction in acd5 points to a role for ceramide phosphate and/or the balance of ceramides in mediating early antifungal responses that are independent of cell death.

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Section: Plants and Materialsmentioning

confidence: 99%

“…For chemical treatments, protoplasts were made according to the procedure of Shen et al (2010). The viability was determined by fluorescein diacetate staining (Liang et al, 2003).…”

Section: Protoplast Isolation and Treatmentsmentioning

confidence: 99%

Loss of Ceramide Kinase in Arabidopsis Impairs Defenses and Promotes Ceramide Accumulation and Mitochondrial H₂O₂ Bursts

Liu

et al. 2014

Plant Cell

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146

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“…MWCNTs (10 mg/L) decreased cell dry weight, viability, chlorophyll content and superoxide dismutase (SOD) activity of Arabidopsis thaliana cell suspension [93]. SWCNTs had adverse effects on protoplasts and leaves through oxidative stress, leading to a certain amount of programmed cell death in Arabidopsis thaliana [110].…”

Section: Fate and Impacts Of Carbon Nanotubes On Plantsmentioning

confidence: 99%

“…[110] Thale cress (Arabidopsis thaliana ecotype Columbia Col-0) 10; 20 and 50 mg/L CNT characterisation: TEM / The three types of CNTs were directly taken up and translocated to roots, stems and leaves functionalisation of CNTs induced strong treatments which are reducing the CNT length. It is thus difficult to determine if functionnalisation or CNT matters most for the toxicity.…”

Section: Fate and Impacts Of Carbon Nanotubes On Plantsmentioning

confidence: 99%

Carbon nanotubes: Impacts and behaviour in the terrestrial ecosystem - A review

Liné

Larue

Flahaut

2017

Carbon

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“…On the other hand, studies have shown that carbon-based nanoparticles such as single walled carbon nanotubes triggered reactive oxygen species (ROS) generation in Arabidopsis and rice (Oryza sativa) (Shen et al, 2010). In addition, multiwall carbon nanotubes have been found to induce gene expression of heat shock protein (HSP) 90 in tomato leaves and roots (Khodakovskaya et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Alginate modifies the physiological impact of CeO2 nanoparticles in corn seedlings cultivated in soil

Zhao

Peralta-Videa

Peng

et al. 2014

Journal of Environmental Sciences

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a b s t r a c tAlginates are naturally occurring components of organic matter in natural soil whose effects on nanoparticles (NPs) toxicity to plants is not well understood. In the present study, corn plants were grown for one month in soil spiked with 400 mg/kg CeO 2 NPs with various alginate concentrations. After one month of growth in the NPs impacted soil, plants were harvested and analyzed for Ce and mineral element concentrations. Chlorophyll concentration and heat shock protein 70, used as biomarkers for oxidative stress, were also evaluated. Results showed that, compared to CeO 2 NPs treatment, alginate at 10, 50, and 100 mg/kg increased Ce concentration in roots by approximately 46%, 38%, and 29% and by 115%, 45%, and 56% in shoots, respectively. CeO 2 NPs without alginate increased Mn accumulation in roots by 34% compared to control. CeO 2 NPs with low and medium alginate increased Mn by ca. 92% respect to NPs without alginate and by ca. 155% respect to control. CeO 2 NPs without/with alginate significantly increased accumulation of Fe and Al in roots. In addition, alginate at 50 mg/kg increased Zn accumulation in roots by 52% compared to control. In shoots, K increased at all NP treatments but the accumulation of other elements was not affected. Alginate enlarged the impact of CeO 2 NPs to corn plants by reducing chlorophyll a content and triggering overexpression of heat shock protein 70.

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Induction of programmed cell death in Arabidopsis and rice by single‐wall carbon nanotubes

Abstract: Consequently, SWCNTs have an adverse effect on protoplasts and leaves through oxidative stress, leading to a certain amount of programmed cell death. Although nanomaterials have great advantages in many respects, the benefits and side effects still need to be assessed carefully.

Cited by 235 publications

References 42 publications

Loss of Ceramide Kinase in Arabidopsis Impairs Defenses and Promotes Ceramide Accumulation and Mitochondrial H₂O₂ Bursts

Loss of Ceramide Kinase in Arabidopsis Impairs Defenses and Promotes Ceramide Accumulation and Mitochondrial H₂O₂ Bursts

Carbon nanotubes: Impacts and behaviour in the terrestrial ecosystem - A review

Alginate modifies the physiological impact of CeO2 nanoparticles in corn seedlings cultivated in soil

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Induction of programmed cell death in Arabidopsis and rice by single‐wall carbon nanotubes

Abstract: Consequently, SWCNTs have an adverse effect on protoplasts and leaves through oxidative stress, leading to a certain amount of programmed cell death. Although nanomaterials have great advantages in many respects, the benefits and side effects still need to be assessed carefully.

Cited by 235 publications

References 42 publications

Loss of Ceramide Kinase in Arabidopsis Impairs Defenses and Promotes Ceramide Accumulation and Mitochondrial H2O2 Bursts

Loss of Ceramide Kinase in Arabidopsis Impairs Defenses and Promotes Ceramide Accumulation and Mitochondrial H2O2 Bursts

Carbon nanotubes: Impacts and behaviour in the terrestrial ecosystem - A review

Alginate modifies the physiological impact of CeO2 nanoparticles in corn seedlings cultivated in soil

Contact Info

Product

Resources

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Loss of Ceramide Kinase in Arabidopsis Impairs Defenses and Promotes Ceramide Accumulation and Mitochondrial H₂O₂ Bursts

Loss of Ceramide Kinase in Arabidopsis Impairs Defenses and Promotes Ceramide Accumulation and Mitochondrial H₂O₂ Bursts